B9KMH8 (SYL_RHOSK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase
EC=6.1.1.4

Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS

Gene names

Name:	leuS
Ordered Locus Names:	RSKD131_2209

Organism

Rhodobacter sphaeroides (strain KD131 / KCTC 12085) [Complete proteome] [HAMAP]

Taxonomic identifier

557760 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter › Rhodobacter sphaeroides

Protein attributes

Sequence length	847 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00049.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: UniProtKB-HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 847	847	Leucine--tRNA ligase HAMAP-Rule MF_00049		PRO_1000199221
Regions
	Motif	41 – 51	11	"HIGH" region HAMAP-Rule MF_00049
	Motif	619 – 623	5	"KMSKS" region HAMAP-Rule MF_00049
Sites
	Binding site	622	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9KMH8 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 342919F87D884627	FASTA	847	94,035
	10 20 30 40 50 60 MSRYDPAATE SRWQAAWDAA GVFTARHDPA RPKYYVLEMF PYPSGRIHMG HVRNYTMGDV 70 80 90 100 110 120 VARQKAAAGF SVLHPMGWDA FGMPAENAAM ERGGHPKDWT YGNIADMRAQ MKPLGLSIDW 130 140 150 160 170 180 SREFATCDPE YYGQQQAMFI DMMEAGLVYR KNAVVNWDPV DMTVLANEQV IDGKGWRSGA 190 200 210 220 230 240 PVVRRELTQW FFRISDYAGE LLEALDTLKD WPEKVRLMQA NWIGQSRGLQ FAFSTAGAPE 250 260 270 280 290 300 GFDRLEVYTT RPDTLMGASF AAISPDHPLA RHLERHDAEV AEFVAECRRV GTSEEALEKA 310 320 330 340 350 360 EKKGFDTGLR VRHPFDTAWE LPVYIANFIL MDYGTGAIFG CPAHDQRDFE FATKYGLPIR 370 380 390 400 410 420 PVFLPEGTEE TALAEAFVPM KSERVHYIRG FAGAEVQSGE EGVAAAIDFC ESQGVGRGVT 430 440 450 460 470 480 NYRLRDWGIS RQRYWGCPIP VIHCETCGVV PEAKENLPVR LPDDVSFDVP GNPLDRHPTW 490 500 510 520 530 540 RDCTCPKCGA KARRETDTMD TFVDSSWYYA RFTAPRAATP TDAEEADYWM NVDQYIGGIE 550 560 570 580 590 600 HAILHLLYSR FFARAMQKTG HLPAKAIEPF NALFTQGMVT HEIYLTRDAA GRPVYHLPED 610 620 630 640 650 660 VTDGKLADGT PVEIIPSAKM SKSKKNVVDP MNIIRQFGAD TARWFVMSDS PPERDVEWTA 670 680 690 700 710 720 SGAEAASKHL HRVWRLADEI SRADGEANAE DGALDKATAR AIAEVTQGVE GFAFNKAIAK 730 740 750 760 770 780 LYEFTNTLSR SGAGAEAKKR AMRTMAQLMS PMVPHLAEEV WAMLGGEGLV AQAAWPKADP 790 800 810 820 830 840 ALLIDDTVTL PIQVNGKRRG EITVPKEMAA SEVEKLVLAD EAVQRALGGA APKKLIVVPG RIVNVVI

References

[1]	"Complete genome sequence of Rhodobacter sphaeroides KD131." Lim S.-K., Kim S.J., Cha S.H., Oh Y.-K., Rhee H.-J., Kim M.-S., Lee J.K. J. Bacteriol. 191:1118-1119(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KD131 / KCTC 12085.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001150 Genomic DNA. Translation: ACM02069.1.
RefSeq	YP_002526570.1. NC_011963.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	557760.RSKD131_2209.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACM02069; ACM02069; RSKD131_2209.
GeneID	7358316.
KEGG	rsk:RSKD131_2209.
PATRIC	23183539. VBIRhoSph125910_3615.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0495.
HOGENOM	HOG000200747.
KO	K01869.
OMA	GIEHACM.
OrthoDB	EOG63Z74X.
ProtClustDB	PRK00390.
Enzyme and pathway databases
BioCyc	RSPH557760:GH1P-2236-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 3 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 3 hits. PF13603. tRNA-synt_1_2. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. SSF47323. 1 hit. SSF50677. SSF50677. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYL_RHOSK

Accession

Primary (citable) accession number: B9KMH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	November 13, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program