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B9KM96

- BIOB_RHOSK

UniProt

B9KM96 - BIOB_RHOSK

Protein

Biotin synthase

Gene

bioB

Organism
Rhodobacter sphaeroides (strain KD131 / KCTC 12085)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi100 – 1001Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi191 – 1911Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi264 – 2641Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciRSPH557760:GH1P-236-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:RSKD131_0234
    OrganismiRhodobacter sphaeroides (strain KD131 / KCTC 12085)
    Taxonomic identifieri557760 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter
    ProteomesiUP000001597: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Biotin synthasePRO_0000381588Add
    BLAST

    Proteomic databases

    PRIDEiB9KM96.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi557760.RSKD131_0234.

    Structurei

    3D structure databases

    ProteinModelPortaliB9KM96.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiCKEDCIF.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B9KM96-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIPSDLRTDW TVEEARAIHA LPFPDLMHRA QTLHRAHFDP TAIETASLLS    50
    IKTGGCPEDC GYCAQSAHHD TGVKATKLMA TEEVLAAARR AKAAGAQRFC 100
    MGAAWRSPKD RDMDRLCDMV QGVAELGLET CMTLGMLSPG QVARLRAAGL 150
    DFYNHNIDTS PAYYARIAST RTMEDRLETV EQVRRGGIKV CCGGILGMGE 200
    AEEDRIALLV TLATLPAHPD SVPVNLWTEI EGVPVQGRAR AVDPFALVRI 250
    VALARILMPA SVLRLSAGRT EMSDELQALC FLAGANSIFV GDQLLTTGNP 300
    AAWKDRDLLT RLGLHVAPAR ARPLVAAN 328
    Length:328
    Mass (Da):35,445
    Last modified:March 24, 2009 - v1
    Checksum:iB13115ACFDDF5EEE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001150 Genomic DNA. Translation: ACM00094.1.
    RefSeqiYP_002524595.1. NC_011963.1.

    Genome annotation databases

    EnsemblBacteriaiACM00094; ACM00094; RSKD131_0234.
    GeneIDi7357696.
    KEGGirsk:RSKD131_0234.
    PATRICi23179566. VBIRhoSph125910_1656.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001150 Genomic DNA. Translation: ACM00094.1 .
    RefSeqi YP_002524595.1. NC_011963.1.

    3D structure databases

    ProteinModelPortali B9KM96.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 557760.RSKD131_0234.

    Proteomic databases

    PRIDEi B9KM96.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM00094 ; ACM00094 ; RSKD131_0234 .
    GeneIDi 7357696.
    KEGGi rsk:RSKD131_0234.
    PATRICi 23179566. VBIRhoSph125910_1656.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi CKEDCIF.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci RSPH557760:GH1P-236-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Rhodobacter sphaeroides KD131."
      Lim S.-K., Kim S.J., Cha S.H., Oh Y.-K., Rhee H.-J., Kim M.-S., Lee J.K.
      J. Bacteriol. 191:1118-1119(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KD131 / KCTC 12085.

    Entry informationi

    Entry nameiBIOB_RHOSK
    AccessioniPrimary (citable) accession number: B9KM96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3