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Protein

Malate dehydrogenase

Gene

mdh

Organism
Anaplasma marginale (strain Florida)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381NADUniRule annotation
Binding sitei87 – 871SubstrateUniRule annotation
Binding sitei93 – 931SubstrateUniRule annotation
Binding sitei100 – 1001NADUniRule annotation
Binding sitei125 – 1251SubstrateUniRule annotation
Binding sitei156 – 1561SubstrateUniRule annotation
Active sitei180 – 1801Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 186NADUniRule annotation
Nucleotide bindingi123 – 1253NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciAMAR320483:GIWE-409-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:AMF_421
OrganismiAnaplasma marginale (strain Florida)
Taxonomic identifieri320483 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma
ProteomesiUP000007307: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Malate dehydrogenasePRO_1000191638Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi320483.AMF_421.

Structurei

3D structure databases

ProteinModelPortaliB9KIH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiVINRVQF.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

B9KIH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSSRSAKVS LVGAGNIGGA LAHMLGASQV VKELVLVDVA GGMTEGKVLD
60 70 80 90 100
VGQALALLGS DVYITGGSDY AAIEHSDAVV VTAGIPRKEG MSREDLLNTN
110 120 130 140 150
AAVVRNIAEN IAKYSPGALV IVVTNPLDAM VWCMYKYSGL PANRVVGMAG
160 170 180 190 200
VLDSARFSFF LARHMNVSVS SVSAMVLGGH GDLMLPLLRY STVGGVPVES
210 220 230 240 250
LIESGRLNRG DIAAIVERTR KGGEEIVKLL KTGSAYCAPA ASCAHMLESY
260 270 280 290 300
VRDKRSIMPC SAYLDGQYGV RDLFVGVPVI IGEKGVEEVV EFPLTAEEQA
310
VFDQSVELIR GSVSAIS
Length:317
Mass (Da):33,320
Last modified:March 24, 2009 - v1
Checksum:i431DC91533609EC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001079 Genomic DNA. Translation: ACM49282.1.
RefSeqiYP_002563538.1. NC_012026.1.

Genome annotation databases

EnsemblBacteriaiACM49282; ACM49282; AMF_421.
GeneIDi7397962.
KEGGiamf:AMF_421.
PATRICi20944710. VBIAnaMar82315_0493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001079 Genomic DNA. Translation: ACM49282.1.
RefSeqiYP_002563538.1. NC_012026.1.

3D structure databases

ProteinModelPortaliB9KIH0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi320483.AMF_421.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM49282; ACM49282; AMF_421.
GeneIDi7397962.
KEGGiamf:AMF_421.
PATRICi20944710. VBIAnaMar82315_0493.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiVINRVQF.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciAMAR320483:GIWE-409-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Conservation in the face of diversity: multistrain analysis of an intracellular bacterium."
    Dark M.J., Herndon D.R., Kappmeyer L.S., Gonzales M.P., Nordeen E., Palmer G.H., Knowles D.P. Jr., Brayton K.A.
    BMC Genomics 10:16-16(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Florida.

Entry informationi

Entry nameiMDH_ANAMF
AccessioniPrimary (citable) accession number: B9KIH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: January 7, 2015
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.