Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B9KIE3

- PNP_ANAMF

UniProt

B9KIE3 - PNP_ANAMF

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Anaplasma marginale (strain Florida)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi491 – 4911MagnesiumUniRule annotation
    Metal bindingi497 – 4971MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciAMAR320483:GIWE-380-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:AMF_391
    OrganismiAnaplasma marginale (strain Florida)
    Taxonomic identifieri320483 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma
    ProteomesiUP000007307: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 805805Polyribonucleotide nucleotidyltransferasePRO_0000381865Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi320483.AMF_391.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini558 – 61760KHUniRule annotationAdd
    BLAST
    Domaini627 – 69468S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218326.
    KOiK00962.
    OrthoDBiEOG6WT8CC.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9KIE3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFDITRKCVE WGDRLLVIES GKIARQADGA VVVDYGGTSV LSTVVSQKSK    50
    EPVDFLPLTV QFLAKSYAIG RIPGGFFKRE GKPSDRETLI SRLVDRSIRP 100
    LFPTGFCDEI VIVCNLLSYD QVSPPETVAL IGAAAALAIS GIPFPTPIAG 150
    AKIGYIREEE RYILNPSAEE LARSELDMFY SGTKSSVVMV ESEASELSEE 200
    EMLGAVTFGH ENCAQVLDLI EEFAEAAGPK DVVEFVPHDI GKVVSDISSG 250
    YSEKFSVAYS DHNKKPECSS LMPPGKACTQ ICAGKHVEES GEYTEQEVLL 300
    AIKTFERSLV RARVLDTLKR VDGRGFDQIR NIEIEVDLIP RSHGSALFTR 350
    GDTQALVITA LGTPQDEQVV DGFDGDRRER FLLHYNFPSY AVGEAAALRP 400
    PGRREIGHGK LAWRAIHPVL PTKADFPYTI RVVSEITESD GSSSMATVCG 450
    ASLALMDTGV PLKSSVAGIA MGLIKEGDRY AVLSDIIGDE DYLGDMDFKV 500
    AGTKDGITAL QMDMKIRGIG FDIIEKSLQQ AKDGRLFIIG KMDKVIKESR 550
    EGVRDHVPRM ESMIIDKNKI KNVIGTGGKN VREICEKTGV KIEISQDGTV 600
    MIYAVSRDAV EEAKNMIMCI VSEPEVGKVF SGVISEIAKY GAFVSFLGGR 650
    RGLVHISEIK NEHIGSVSDV LAVDDKVKVL VIGIDKDHVQ LSMRRVDQDS 700
    GDLLEHESYS SNKKNGPQFG DASGGASGFR DYASGPARER RRSGGSGGRP 750
    VRRRSAGSSG SGSGGCYSVP QHVGTPDPVH GNDRRRGSGP QHASGGGGNK 800
    KPRFF 805
    Length:805
    Mass (Da):87,147
    Last modified:March 24, 2009 - v1
    Checksum:i58EB5A9260C4E15A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001079 Genomic DNA. Translation: ACM49255.1.
    RefSeqiWP_012658930.1. NC_012026.1.
    YP_002563511.1. NC_012026.1.

    Genome annotation databases

    EnsemblBacteriaiACM49255; ACM49255; AMF_391.
    GeneIDi7397935.
    KEGGiamf:AMF_391.
    PATRICi20944632. VBIAnaMar82315_0455.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001079 Genomic DNA. Translation: ACM49255.1 .
    RefSeqi WP_012658930.1. NC_012026.1.
    YP_002563511.1. NC_012026.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 320483.AMF_391.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM49255 ; ACM49255 ; AMF_391 .
    GeneIDi 7397935.
    KEGGi amf:AMF_391.
    PATRICi 20944632. VBIAnaMar82315_0455.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218326.
    KOi K00962.
    OrthoDBi EOG6WT8CC.

    Enzyme and pathway databases

    BioCyci AMAR320483:GIWE-380-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Conservation in the face of diversity: multistrain analysis of an intracellular bacterium."
      Dark M.J., Herndon D.R., Kappmeyer L.S., Gonzales M.P., Nordeen E., Palmer G.H., Knowles D.P. Jr., Brayton K.A.
      BMC Genomics 10:16-16(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Florida.

    Entry informationi

    Entry nameiPNP_ANAMF
    AccessioniPrimary (citable) accession number: B9KIE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3