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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Anaplasma marginale (strain Florida)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi491MagnesiumUniRule annotation1
Metal bindingi497MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:AMF_391
OrganismiAnaplasma marginale (strain Florida)
Taxonomic identifieri320483 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma
Proteomesi
  • UP000007307 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003818651 – 805Polyribonucleotide nucleotidyltransferaseAdd BLAST805

Proteomic databases

PRIDEiB9KIE3.

Interactioni

Protein-protein interaction databases

STRINGi320483.AMF_391.

Structurei

3D structure databases

SMRiB9KIE3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini558 – 617KHUniRule annotationAdd BLAST60
Domaini627 – 694S1 motifUniRule annotationAdd BLAST68

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRYMHNYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

B9KIE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDITRKCVE WGDRLLVIES GKIARQADGA VVVDYGGTSV LSTVVSQKSK
60 70 80 90 100
EPVDFLPLTV QFLAKSYAIG RIPGGFFKRE GKPSDRETLI SRLVDRSIRP
110 120 130 140 150
LFPTGFCDEI VIVCNLLSYD QVSPPETVAL IGAAAALAIS GIPFPTPIAG
160 170 180 190 200
AKIGYIREEE RYILNPSAEE LARSELDMFY SGTKSSVVMV ESEASELSEE
210 220 230 240 250
EMLGAVTFGH ENCAQVLDLI EEFAEAAGPK DVVEFVPHDI GKVVSDISSG
260 270 280 290 300
YSEKFSVAYS DHNKKPECSS LMPPGKACTQ ICAGKHVEES GEYTEQEVLL
310 320 330 340 350
AIKTFERSLV RARVLDTLKR VDGRGFDQIR NIEIEVDLIP RSHGSALFTR
360 370 380 390 400
GDTQALVITA LGTPQDEQVV DGFDGDRRER FLLHYNFPSY AVGEAAALRP
410 420 430 440 450
PGRREIGHGK LAWRAIHPVL PTKADFPYTI RVVSEITESD GSSSMATVCG
460 470 480 490 500
ASLALMDTGV PLKSSVAGIA MGLIKEGDRY AVLSDIIGDE DYLGDMDFKV
510 520 530 540 550
AGTKDGITAL QMDMKIRGIG FDIIEKSLQQ AKDGRLFIIG KMDKVIKESR
560 570 580 590 600
EGVRDHVPRM ESMIIDKNKI KNVIGTGGKN VREICEKTGV KIEISQDGTV
610 620 630 640 650
MIYAVSRDAV EEAKNMIMCI VSEPEVGKVF SGVISEIAKY GAFVSFLGGR
660 670 680 690 700
RGLVHISEIK NEHIGSVSDV LAVDDKVKVL VIGIDKDHVQ LSMRRVDQDS
710 720 730 740 750
GDLLEHESYS SNKKNGPQFG DASGGASGFR DYASGPARER RRSGGSGGRP
760 770 780 790 800
VRRRSAGSSG SGSGGCYSVP QHVGTPDPVH GNDRRRGSGP QHASGGGGNK

KPRFF
Length:805
Mass (Da):87,147
Last modified:March 24, 2009 - v1
Checksum:i58EB5A9260C4E15A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001079 Genomic DNA. Translation: ACM49255.1.

Genome annotation databases

EnsemblBacteriaiACM49255; ACM49255; AMF_391.
KEGGiamf:AMF_391.
PATRICifig|320483.3.peg.455.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPNP_ANAMF
AccessioniPrimary (citable) accession number: B9KIE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: March 24, 2009
Last modified: July 5, 2017
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families