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B9KH95 (RUVC_ANAMF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:AMF_981
OrganismAnaplasma marginale (strain Florida) [Complete proteome] [HAMAP]
Taxonomic identifier320483 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma

Protein attributes

Sequence length165 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 165165Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000195233

Sites

Metal binding71Magnesium By similarity
Metal binding681Magnesium By similarity
Metal binding1421Magnesium By similarity
Metal binding1451Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
B9KH95 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 91C36F62A239B67C

FASTA16518,007
        10         20         30         40         50         60 
MMIIGIDPGL EFTGWGVVSS TNSQSVCLLD SGVISTRGIS RESEKLYKIY VSLLSVLSLY 

        70         80         90        100        110        120 
KIDEASIEKV FINSNPRSSM SLCYARAAST ISVMSRGIDI YEYSSTAIKK CITGSGMAPK 

       130        140        150        160 
EQVSFMVRSS LGIKQDVEIN NHSSDAIAAA LCHVYNTRNR NFALK 

« Hide

References

[1]"Conservation in the face of diversity: multistrain analysis of an intracellular bacterium."
Dark M.J., Herndon D.R., Kappmeyer L.S., Gonzales M.P., Nordeen E., Palmer G.H., Knowles D.P. Jr., Brayton K.A.
BMC Genomics 10:16-16(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Florida.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001079 Genomic DNA. Translation: ACM49799.1.
RefSeqYP_002564055.1. NC_012026.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING320483.AMF_981.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM49799; ACM49799; AMF_981.
GeneID7398568.
KEGGamf:AMF_981.
PATRIC20946002. VBIAnaMar82315_1125.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OrthoDBEOG6RG044.
ProtClustDBCLSK747421.

Enzyme and pathway databases

BioCycAMAR320483:GIWE-947-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUVC_ANAMF
AccessionPrimary (citable) accession number: B9KH95
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: April 16, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families