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B9KGM2

- BIOB_ANAMF

UniProt

B9KGM2 - BIOB_ANAMF

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Protein

Biotin synthase

Gene

bioB

Organism
Anaplasma marginale (strain Florida)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi96 – 961Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi127 – 1271Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi187 – 1871Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi259 – 2591Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAMAR320483:GIWE-713-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:AMF_742
OrganismiAnaplasma marginale (strain Florida)
Taxonomic identifieri320483 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma
ProteomesiUP000007307: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Biotin synthasePRO_0000381198Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi320483.AMF_742.

Structurei

3D structure databases

ProteinModelPortaliB9KGM2.
SMRiB9KGM2. Positions 3-313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B9KGM2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIRNNWTLEE ALELFRMPFS DLILKAHSVH VQNFRNNEVQ VAALMNIKTG
60 70 80 90 100
SCPENCRYCA QSAHYNTGLE KKSLSTVEEV KTAAKRAKEI GADRFCFAAA
110 120 130 140 150
WRNLHDRDLE KICQFVEAIK SEGLESCASL GMLKLDQAQK LKESGLDFYN
160 170 180 190 200
HNVDTSREFY HNVVTTRTYE ERLETVRNVQ QAGIKVCCGG ILGMGESTED
210 220 230 240 250
RASMLVTLAN LEQHPLSVPI NRLVPIEGTP MEGNPKIDNI DFVRTIAVAR
260 270 280 290 300
IMMPASYVRL AAGRGEMSEE MQALCMLAGA NSIFCGEKLL TTPNARPEDD
310 320
QRLFSQLGIT PSRAACTTSD AQLA
Length:324
Mass (Da):36,039
Last modified:March 24, 2009 - v1
Checksum:i264C5DEC6C28DAAA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001079 Genomic DNA. Translation: ACM49576.1.
RefSeqiYP_002563832.1. NC_012026.1.

Genome annotation databases

EnsemblBacteriaiACM49576; ACM49576; AMF_742.
GeneIDi7397922.
KEGGiamf:AMF_742.
PATRICi20945434. VBIAnaMar82315_0846.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001079 Genomic DNA. Translation: ACM49576.1 .
RefSeqi YP_002563832.1. NC_012026.1.

3D structure databases

ProteinModelPortali B9KGM2.
SMRi B9KGM2. Positions 3-313.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 320483.AMF_742.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM49576 ; ACM49576 ; AMF_742 .
GeneIDi 7397922.
KEGGi amf:AMF_742.
PATRICi 20945434. VBIAnaMar82315_0846.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci AMAR320483:GIWE-713-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Conservation in the face of diversity: multistrain analysis of an intracellular bacterium."
    Dark M.J., Herndon D.R., Kappmeyer L.S., Gonzales M.P., Nordeen E., Palmer G.H., Knowles D.P. Jr., Brayton K.A.
    BMC Genomics 10:16-16(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Florida.

Entry informationi

Entry nameiBIOB_ANAMF
AccessioniPrimary (citable) accession number: B9KGM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: November 26, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3