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B9KGM2

- BIOB_ANAMF

UniProt

B9KGM2 - BIOB_ANAMF

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Protein

Biotin synthase

Gene
bioB, AMF_742
Organism
Anaplasma marginale (strain Florida)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi52 – 521Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi56 – 561Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi59 – 591Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi96 – 961Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi127 – 1271Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi187 – 1871Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi259 – 2591Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAMAR320483:GIWE-713-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:AMF_742
OrganismiAnaplasma marginale (strain Florida)
Taxonomic identifieri320483 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeAnaplasma
ProteomesiUP000007307: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Biotin synthaseUniRule annotationPRO_0000381198Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi320483.AMF_742.

Structurei

3D structure databases

ProteinModelPortaliB9KGM2.
SMRiB9KGM2. Positions 3-313.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B9KGM2-1 [UniParc]FASTAAdd to Basket

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MIRNNWTLEE ALELFRMPFS DLILKAHSVH VQNFRNNEVQ VAALMNIKTG    50
SCPENCRYCA QSAHYNTGLE KKSLSTVEEV KTAAKRAKEI GADRFCFAAA 100
WRNLHDRDLE KICQFVEAIK SEGLESCASL GMLKLDQAQK LKESGLDFYN 150
HNVDTSREFY HNVVTTRTYE ERLETVRNVQ QAGIKVCCGG ILGMGESTED 200
RASMLVTLAN LEQHPLSVPI NRLVPIEGTP MEGNPKIDNI DFVRTIAVAR 250
IMMPASYVRL AAGRGEMSEE MQALCMLAGA NSIFCGEKLL TTPNARPEDD 300
QRLFSQLGIT PSRAACTTSD AQLA 324
Length:324
Mass (Da):36,039
Last modified:March 24, 2009 - v1
Checksum:i264C5DEC6C28DAAA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001079 Genomic DNA. Translation: ACM49576.1.
RefSeqiYP_002563832.1. NC_012026.1.

Genome annotation databases

EnsemblBacteriaiACM49576; ACM49576; AMF_742.
GeneIDi7397922.
KEGGiamf:AMF_742.
PATRICi20945434. VBIAnaMar82315_0846.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001079 Genomic DNA. Translation: ACM49576.1 .
RefSeqi YP_002563832.1. NC_012026.1.

3D structure databases

ProteinModelPortali B9KGM2.
SMRi B9KGM2. Positions 3-313.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 320483.AMF_742.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM49576 ; ACM49576 ; AMF_742 .
GeneIDi 7397922.
KEGGi amf:AMF_742.
PATRICi 20945434. VBIAnaMar82315_0846.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci AMAR320483:GIWE-713-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Conservation in the face of diversity: multistrain analysis of an intracellular bacterium."
    Dark M.J., Herndon D.R., Kappmeyer L.S., Gonzales M.P., Nordeen E., Palmer G.H., Knowles D.P. Jr., Brayton K.A.
    BMC Genomics 10:16-16(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Florida.

Entry informationi

Entry nameiBIOB_ANAMF
AccessioniPrimary (citable) accession number: B9KGM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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