B9KFP6 (PURL_CAMLR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Cla_0547

Organism

Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) [Complete proteome] [HAMAP]

Taxonomic identifier

306263 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter › Campylobacter lari

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_1000134897
Regions
	Nucleotide binding	99 – 110	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B9KFP6 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 15ACBD6063BEF126	FASTA	725	78,991
	10 20 30 40 50 60 MDKEIIKQHK ISDEEYQEIL NILGREPNLL ELGVISAMWS EHCSYKSSKK YLSGFPTKAP 70 80 90 100 110 120 WVIQGPGENA GVIDIGKKMA AVFKVESHNH PSFIEPFAGA ATGVGGILRD VFTMGARVVA 130 140 150 160 170 180 GMNSLKFGNI HDEKIGKHQK YLVKGVVSGI SHYGNCMGVP TIGGECAFDE CFNGNILVNA 190 200 210 220 230 240 FALGTCKIKD IFYAKAEGIG NPVIYVGSKT GRDGLGGAVM ASDSFNESSK SLRPTVQIGD 250 260 270 280 290 300 PFAEKLLMEA CLELFKTDYI VGIQDMGAAG LTSSSFEMAG RSGSGMRLYL DKTPMREEGM 310 320 330 340 350 360 TPYELMLSES QERMLICAKK GYEEKVIEIF NKWGLDAAII GEVTDSGKME LFWHGELVGL 370 380 390 400 410 420 IPIEPLSEKA PILDRPVARP KYLDEIKNYK FNLNISTQEA FEKLLANENV SNKAYIYEQF 430 440 450 460 470 480 DSSVQTNTLK SDGALGANSI RIKENNCLLS MAIECNSRLN YVNPKIGAAA AVASVGRKIA 490 500 510 520 530 540 CSGAKPLAIS DCLNYGNPQN PEVMWQFAQG CEGIKLACKE LNTPVVSGNV SLYNETDGVS 550 560 570 580 590 600 IFPSPTIACV GVNEKAENVL KSYFSKDTSA IYLLGESKGS FGGSLIAKIL DKKVAGELED 610 620 630 640 650 660 IDFSAELKLW DFLLKANEAK ILDCANSIGI GGIAITLAKM CAMANLGINA KTNFADKSFI 670 680 690 700 710 720 FEESPTRVIV GVKDEEEFIK FVNEMGINFA KLGNLDEKDF ILDDIKISLA KLQTIYFDKF NEYLG

References

[1]

"The complete genome sequence and analysis of the human pathogen Campylobacter lari." Miller W.G., Wang G., Binnewies T.T., Parker C.T. Foodborne Pathog. Dis. 5:371-386(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM2100 / D67 / ATCC BAA-1060.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000932 Genomic DNA. Translation: ACM63881.1.
RefSeq	YP_002575132.1. NC_012039.1.
3D structure databases
ProteinModelPortal	B9KFP6.
ModBase	Search...
Protein-protein interaction databases
STRING	306263.Cla_0547.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACM63881; ACM63881; Cla_0547.
GeneID	7411107.
KEGG	cla:Cla_0547.
PATRIC	20061557. VBICamLar15090_0530.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HOG000238227.
KO	K01952.
OMA	WSEHCCY.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	CLAR306263:GH7X-589-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_CAMLR

Accession

Primary (citable) accession number: B9KFP6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program