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B9KD44

- SYI_CAMLR

UniProt

B9KD44 - SYI_CAMLR

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
    Binding sitei613 – 6131ATPUniRule annotation
    Metal bindingi894 – 8941ZincUniRule annotation
    Metal bindingi897 – 8971ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation
    Metal bindingi912 – 9121ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCLAR306263:GH7X-1159-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Cla_1161
    OrganismiCampylobacter lari (strain RM2100 / D67 / ATCC BAA-1060)
    Taxonomic identifieri306263 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000007727: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 921921Isoleucine--tRNA ligasePRO_1000189141Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi306263.Cla_1161.

    Structurei

    3D structure databases

    ProteinModelPortaliB9KD44.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi610 – 6145"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9KD44-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKDTLLLP NTTFAMRANL AELEPKRFDK WFENNYAYEK MKQKRQGVSE    50
    SFTLHDGPPY ANGHLHIGHA LNKILKDIII KMHYFQGKKV RFTPGWDCHG 100
    LPIEQQVEVK LKDKKQNLSK KQIRELCREH AREFVNIQRD EFKSLGVIAD 150
    WDEPYLTMKN AFEADIYKAL CKIAKKGLLL ERSKPVFWSW AAKSALAEAE 200
    VEYEEKEDYS IYVAFNLDEA SCKKLGVENA KAVIWTTTPW TLPANQAISL 250
    NPNEKYIITK EGYIFAKALL ENMINKNFTQ GEIQKELLGS EFENLSAINP 300
    LNQRKSTLIL GEHVLMDGGT GLVHTAPGHG EDDYYACLKY NIEVIMPVDD 350
    GGYYDETLRA KGLLPEHLLA EFIGLHIFKA NERILELLGE ALLESSKFTH 400
    SYPFCWRTHK PVIYRATKQW FILMDEKKLD GKSLRELALE QLNSVKFYPE 450
    SGVKRLSSMI ENRPDWCISR QRDWGVPIAF FRDKKSQEVI FDDDVLDHLV 500
    GIFEKNGADA WWDLEIKDLL PPNSKYNPNN LEKVYDILDV WFDSGSTWEA 550
    VLNSARYDAG EYQASMYLEG SDQHRGWFQS SLLISTAINH KTPYKNILTH 600
    GFTVDEKGQK MSKSKGNVVL PQNVAKNYGV EILRLWIMLS DYSTDLKISD 650
    NILKQVSEQY RKIRNTIRFL LANTNDIEFV ETKNFTLLDK WILMRAKIAF 700
    EICENAFEKY EFSKGFSVLL NFLSADLSGI YLDICKDRLY CNAKDDSKRV 750
    SAQSAMVLIA RKLFALLAPS LTYTIDEALE HANVAIKENA KDVFDLLNKQ 800
    GFEYEYKIED ELFIKSREKF FEIIDGLKKD KIIKSTLELS LQTSANELLS 850
    EDLEEIADWF MVSVVESIDE QKALAEFKID NIGFKIVKSS LNKCPRCWKF 900
    LAKEDGCLCP RCNGVEKAKN V 921
    Length:921
    Mass (Da):106,124
    Last modified:March 24, 2009 - v1
    Checksum:i16261B95FDBB0591
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000932 Genomic DNA. Translation: ACM64483.1.
    RefSeqiYP_002575734.1. NC_012039.1.

    Genome annotation databases

    EnsemblBacteriaiACM64483; ACM64483; Cla_1161.
    GeneIDi7410325.
    KEGGicla:Cla_1161.
    PATRICi20062809. VBICamLar15090_1149.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000932 Genomic DNA. Translation: ACM64483.1 .
    RefSeqi YP_002575734.1. NC_012039.1.

    3D structure databases

    ProteinModelPortali B9KD44.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 306263.Cla_1161.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM64483 ; ACM64483 ; Cla_1161 .
    GeneIDi 7410325.
    KEGGi cla:Cla_1161.
    PATRICi 20062809. VBICamLar15090_1149.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CLAR306263:GH7X-1159-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence and analysis of the human pathogen Campylobacter lari."
      Miller W.G., Wang G., Binnewies T.T., Parker C.T.
      Foodborne Pathog. Dis. 5:371-386(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RM2100 / D67 / ATCC BAA-1060.

    Entry informationi

    Entry nameiSYI_CAMLR
    AccessioniPrimary (citable) accession number: B9KD44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3