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B9KCS8 (SYL_CAMLR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Cla_1045
OrganismCampylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) [Complete proteome] [HAMAP]
Taxonomic identifier306263 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length809 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 809809Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199186

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif579 – 5835"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5821ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B9KCS8 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: AE1DA80F77FDCD12

FASTA80993,357
        10         20         30         40         50         60 
MAYEAGKIEK KWQKIWQEKE YFEPKDDFSL PKKYILSMFP YPSGRIHMGH VRNYSIGDAM 

        70         80         90        100        110        120 
ARYYRKKGFN VLHPIGFDSF GMPAENAAIK HGIHPKKWTY ENIDYMQNEL ASLGFSFSKK 

       130        140        150        160        170        180 
RMLATSDPLY TKFEQEFFIK MYEKGLIYTK EAEVNWCEND KTVLANEQVE DGKCWRCGHE 

       190        200        210        220        230        240 
VIRKKMPGYY VKITAYADEL LQDLKKLEGK WPSQVLTMQE NWIGKSTGLS FDFDIEENNK 

       250        260        270        280        290        300 
ISAKKINVFT TRAETIYGVS YIALAPDHEI VNELIDKKLL DQDVIAKIQN IQNQTPRQRQ 

       310        320        330        340        350        360 
AAPKEGYFLN LYVIHPLSKE KIPLWVANFV LSDYGSGAVM SVPAHDERDY EFAKTYNLAI 

       370        380        390        400        410        420 
KKVIYKDEND AQCYTLKEGV LTSSGEFDQL ECNDAREKIS LKIESLGIGK KVTNFKIRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMIKCDSC GIVPQKIENL PITLPEDVVI NGEGNPLDKH ETWKECICPK 

       490        500        510        520        530        540 
CGKKAQKESD TLDTFFESSW YFARFASDDK TWQEKAVDEK SVNYWMNVDE YIGGIEHAIL 

       550        560        570        580        590        600 
HLLYARFFQK ALRDLGYLKD DEPFNRLLTQ GMVTKDGAKM SKSKGNVVDP DYIIEKYGAD 

       610        620        630        640        650        660 
SARLFILFAA PPAKELEWND SALEGAFKFI NRLYEKAMSL ECGKLQEIDH QSLNKEEKYA 

       670        680        690        700        710        720 
RLKVYEALKK SFEVYEESFA FNTLIAACME ALNALNAINH KEVSKEAFYI ILNILEPIIP 

       730        740        750        760        770        780 
HVCFELSEYL FKCENFKVLK IKDEVFIKDS FNIAISVNGK KRAQIEINSE AKEDEILALA 

       790        800 
KENVAKWLEG KTIVKEIYID KKLVNLVVK 

« Hide

References

[1]"The complete genome sequence and analysis of the human pathogen Campylobacter lari."
Miller W.G., Wang G., Binnewies T.T., Parker C.T.
Foodborne Pathog. Dis. 5:371-386(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RM2100 / D67 / ATCC BAA-1060.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000932 Genomic DNA. Translation: ACM64367.1.
RefSeqYP_002575618.1. NC_012039.1.

3D structure databases

ProteinModelPortalB9KCS8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING306263.Cla_1045.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM64367; ACM64367; Cla_1045.
GeneID7410779.
KEGGcla:Cla_1045.
PATRIC20062569. VBICamLar15090_1029.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAVVHCDAC.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCLAR306263:GH7X-1043-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CAMLR
AccessionPrimary (citable) accession number: B9KCS8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries