Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B9KCQ2 (NAPA_CAMLR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Cla_1018
OrganismCampylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) [Complete proteome] [HAMAP]
Taxonomic identifier306263 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length924 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 924895Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_1000186351

Regions

Domain35 – 91574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding421Iron-sulfur (4Fe-4S) By similarity
Metal binding451Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding771Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B9KCQ2 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 9CC5CCEFF076637A

FASTA924104,803
        10         20         30         40         50         60 
MNRRDFIKNT AIASACGVAG LSVPSSVLAN TENKWRWDKA VCRFCGTGCG ILVARQDGKI 

        70         80         90        100        110        120 
VAVKGDPAAP VNRGLNCIKG YFNAKIMYGE DRLVTPLLRV NAKGEFDKNG KFQQVSWQRA 

       130        140        150        160        170        180 
FDEMEKHFKK AYKEKGVEGI GIFASGQYTI QEGYAAAKLV KAGFRSNNID PNARHCMASA 

       190        200        210        220        230        240 
VVGFMQTFGV DEPSGCYDDI ELTDTIITWG ANMAEMHPIL WSRVSDRKLS NLDKVKIVNL 

       250        260        270        280        290        300 
STFSNRTSHI ADTEIIFKPN TDLAIWNYIA REIVYNHPEA MDKEFVEKHC IFTTGYADIG 

       310        320        330        340        350        360 
YGMRNNPNHP KFKASEKDTV AKQNSIILDD EEAVSLAYLG VKAGDKFEMK HQSAPDAHWE 

       370        380        390        400        410        420 
ISFEDFKKAL EPYTLDYVAK VAKGNEDESI EEFKKKLQEL ANLYIEKNRK VVSFWTMGFN 

       430        440        450        460        470        480 
QHTRGSWVNE QAYMVHFLLG KQAKPGSGAF SLTGQPSACG TAREVGTFSH RLPADMVVAN 

       490        500        510        520        530        540 
PKHREISEKI WKVPAKTINP KPGAPYLKIM RDLEDGNIKF AWVQVNNPWQ NTANANHWIA 

       550        560        570        580        590        600 
AAREMDNFIV VSDCYPGISA KVADLILPSA MIYEKWGAYG NAERRTQHWK QQVLPVGEAM 

       610        620        630        640        650        660 
SDTWQILEFA KRFKLKEVWG ETKVDDKLTL PSILEEAKAM GYNEDDTLYD VLFANKEAKS 

       670        680        690        700        710        720 
FKANDPIAKG FDNSDVNGDE RKIIGSDGKE FDGYGFFVQK YLWEEYRKFG LGHGHDLADF 

       730        740        750        760        770        780 
DTYHKVRGLR WPVVNGKETQ WRFNTKFDYY AKKAAPNSDF AFYGAFAKEL PKGDLIAPQT 

       790        800        810        820        830        840 
QEKYSLKNKA KIFFRPFMKA PERPSEEYPF WLCTGRVLEH WHSGTMTMRV PELFRAVPEA 

       850        860        870        880        890        900 
LCYMNEDDAK KMQIAQGDIV WVESRRGKVK ARVDFRGRNK PSKGLVYVPW FDENVYINKV 

       910        920 
TLDATCPLSN QTDFKKCAVK ITKA 

« Hide

References

[1]"The complete genome sequence and analysis of the human pathogen Campylobacter lari."
Miller W.G., Wang G., Binnewies T.T., Parker C.T.
Foodborne Pathog. Dis. 5:371-386(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: RM2100 / D67 / ATCC BAA-1060.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000932 Genomic DNA. Translation: ACM64341.1.
RefSeqYP_002575592.1. NC_012039.1.

3D structure databases

ProteinModelPortalB9KCQ2.
SMRB9KCQ2. Positions 35-923.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING306263.Cla_1018.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM64341; ACM64341; Cla_1018.
GeneID7410753.
KEGGcla:Cla_1018.
PATRIC20062515. VBICamLar15090_1002.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMARRTHVWH.
OrthoDBEOG6CVV7G.
ProtClustDBPRK13532.

Enzyme and pathway databases

BioCycCLAR306263:GH7X-1017-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_CAMLR
AccessionPrimary (citable) accession number: B9KCQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families