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B9KCQ2

- NAPA_CAMLR

UniProt

B9KCQ2 - NAPA_CAMLR

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi42 – 421Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi45 – 451Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi77 – 771Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciCLAR306263:GH7X-1017-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:Cla_1018
    OrganismiCampylobacter lari (strain RM2100 / D67 / ATCC BAA-1060)
    Taxonomic identifieri306263 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000007727: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
    BLAST
    Chaini30 – 924895Periplasmic nitrate reductasePRO_1000186351Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi306263.Cla_1018.

    Structurei

    3D structure databases

    ProteinModelPortaliB9KCQ2.
    SMRiB9KCQ2. Positions 35-923.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 91574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiHPKTRVA.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B9KCQ2-1 [UniParc]FASTAAdd to Basket

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    MNRRDFIKNT AIASACGVAG LSVPSSVLAN TENKWRWDKA VCRFCGTGCG    50
    ILVARQDGKI VAVKGDPAAP VNRGLNCIKG YFNAKIMYGE DRLVTPLLRV 100
    NAKGEFDKNG KFQQVSWQRA FDEMEKHFKK AYKEKGVEGI GIFASGQYTI 150
    QEGYAAAKLV KAGFRSNNID PNARHCMASA VVGFMQTFGV DEPSGCYDDI 200
    ELTDTIITWG ANMAEMHPIL WSRVSDRKLS NLDKVKIVNL STFSNRTSHI 250
    ADTEIIFKPN TDLAIWNYIA REIVYNHPEA MDKEFVEKHC IFTTGYADIG 300
    YGMRNNPNHP KFKASEKDTV AKQNSIILDD EEAVSLAYLG VKAGDKFEMK 350
    HQSAPDAHWE ISFEDFKKAL EPYTLDYVAK VAKGNEDESI EEFKKKLQEL 400
    ANLYIEKNRK VVSFWTMGFN QHTRGSWVNE QAYMVHFLLG KQAKPGSGAF 450
    SLTGQPSACG TAREVGTFSH RLPADMVVAN PKHREISEKI WKVPAKTINP 500
    KPGAPYLKIM RDLEDGNIKF AWVQVNNPWQ NTANANHWIA AAREMDNFIV 550
    VSDCYPGISA KVADLILPSA MIYEKWGAYG NAERRTQHWK QQVLPVGEAM 600
    SDTWQILEFA KRFKLKEVWG ETKVDDKLTL PSILEEAKAM GYNEDDTLYD 650
    VLFANKEAKS FKANDPIAKG FDNSDVNGDE RKIIGSDGKE FDGYGFFVQK 700
    YLWEEYRKFG LGHGHDLADF DTYHKVRGLR WPVVNGKETQ WRFNTKFDYY 750
    AKKAAPNSDF AFYGAFAKEL PKGDLIAPQT QEKYSLKNKA KIFFRPFMKA 800
    PERPSEEYPF WLCTGRVLEH WHSGTMTMRV PELFRAVPEA LCYMNEDDAK 850
    KMQIAQGDIV WVESRRGKVK ARVDFRGRNK PSKGLVYVPW FDENVYINKV 900
    TLDATCPLSN QTDFKKCAVK ITKA 924
    Length:924
    Mass (Da):104,803
    Last modified:March 24, 2009 - v1
    Checksum:i9CC5CCEFF076637A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000932 Genomic DNA. Translation: ACM64341.1.
    RefSeqiWP_012661724.1. NC_012039.1.
    YP_002575592.1. NC_012039.1.

    Genome annotation databases

    EnsemblBacteriaiACM64341; ACM64341; Cla_1018.
    GeneIDi7410753.
    KEGGicla:Cla_1018.
    PATRICi20062515. VBICamLar15090_1002.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000932 Genomic DNA. Translation: ACM64341.1 .
    RefSeqi WP_012661724.1. NC_012039.1.
    YP_002575592.1. NC_012039.1.

    3D structure databases

    ProteinModelPortali B9KCQ2.
    SMRi B9KCQ2. Positions 35-923.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 306263.Cla_1018.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM64341 ; ACM64341 ; Cla_1018 .
    GeneIDi 7410753.
    KEGGi cla:Cla_1018.
    PATRICi 20062515. VBICamLar15090_1002.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi HPKTRVA.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci CLAR306263:GH7X-1017-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence and analysis of the human pathogen Campylobacter lari."
      Miller W.G., Wang G., Binnewies T.T., Parker C.T.
      Foodborne Pathog. Dis. 5:371-386(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: RM2100 / D67 / ATCC BAA-1060.

    Entry informationi

    Entry nameiNAPA_CAMLR
    AccessioniPrimary (citable) accession number: B9KCQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3