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B9KCQ2

- NAPA_CAMLR

UniProt

B9KCQ2 - NAPA_CAMLR

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi45 – 451Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi77 – 771Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciCLAR306263:GH7X-1017-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Cla_1018
OrganismiCampylobacter lari (strain RM2100 / D67 / ATCC BAA-1060)
Taxonomic identifieri306263 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000007727: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
BLAST
Chaini30 – 924895Periplasmic nitrate reductasePRO_1000186351Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi306263.Cla_1018.

Structurei

3D structure databases

ProteinModelPortaliB9KCQ2.
SMRiB9KCQ2. Positions 35-923.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 91574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B9KCQ2-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MNRRDFIKNT AIASACGVAG LSVPSSVLAN TENKWRWDKA VCRFCGTGCG
60 70 80 90 100
ILVARQDGKI VAVKGDPAAP VNRGLNCIKG YFNAKIMYGE DRLVTPLLRV
110 120 130 140 150
NAKGEFDKNG KFQQVSWQRA FDEMEKHFKK AYKEKGVEGI GIFASGQYTI
160 170 180 190 200
QEGYAAAKLV KAGFRSNNID PNARHCMASA VVGFMQTFGV DEPSGCYDDI
210 220 230 240 250
ELTDTIITWG ANMAEMHPIL WSRVSDRKLS NLDKVKIVNL STFSNRTSHI
260 270 280 290 300
ADTEIIFKPN TDLAIWNYIA REIVYNHPEA MDKEFVEKHC IFTTGYADIG
310 320 330 340 350
YGMRNNPNHP KFKASEKDTV AKQNSIILDD EEAVSLAYLG VKAGDKFEMK
360 370 380 390 400
HQSAPDAHWE ISFEDFKKAL EPYTLDYVAK VAKGNEDESI EEFKKKLQEL
410 420 430 440 450
ANLYIEKNRK VVSFWTMGFN QHTRGSWVNE QAYMVHFLLG KQAKPGSGAF
460 470 480 490 500
SLTGQPSACG TAREVGTFSH RLPADMVVAN PKHREISEKI WKVPAKTINP
510 520 530 540 550
KPGAPYLKIM RDLEDGNIKF AWVQVNNPWQ NTANANHWIA AAREMDNFIV
560 570 580 590 600
VSDCYPGISA KVADLILPSA MIYEKWGAYG NAERRTQHWK QQVLPVGEAM
610 620 630 640 650
SDTWQILEFA KRFKLKEVWG ETKVDDKLTL PSILEEAKAM GYNEDDTLYD
660 670 680 690 700
VLFANKEAKS FKANDPIAKG FDNSDVNGDE RKIIGSDGKE FDGYGFFVQK
710 720 730 740 750
YLWEEYRKFG LGHGHDLADF DTYHKVRGLR WPVVNGKETQ WRFNTKFDYY
760 770 780 790 800
AKKAAPNSDF AFYGAFAKEL PKGDLIAPQT QEKYSLKNKA KIFFRPFMKA
810 820 830 840 850
PERPSEEYPF WLCTGRVLEH WHSGTMTMRV PELFRAVPEA LCYMNEDDAK
860 870 880 890 900
KMQIAQGDIV WVESRRGKVK ARVDFRGRNK PSKGLVYVPW FDENVYINKV
910 920
TLDATCPLSN QTDFKKCAVK ITKA
Length:924
Mass (Da):104,803
Last modified:March 24, 2009 - v1
Checksum:i9CC5CCEFF076637A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000932 Genomic DNA. Translation: ACM64341.1.
RefSeqiWP_012661724.1. NC_012039.1.
YP_002575592.1. NC_012039.1.

Genome annotation databases

EnsemblBacteriaiACM64341; ACM64341; Cla_1018.
GeneIDi7410753.
KEGGicla:Cla_1018.
PATRICi20062515. VBICamLar15090_1002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000932 Genomic DNA. Translation: ACM64341.1 .
RefSeqi WP_012661724.1. NC_012039.1.
YP_002575592.1. NC_012039.1.

3D structure databases

ProteinModelPortali B9KCQ2.
SMRi B9KCQ2. Positions 35-923.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 306263.Cla_1018.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM64341 ; ACM64341 ; Cla_1018 .
GeneIDi 7410753.
KEGGi cla:Cla_1018.
PATRICi 20062515. VBICamLar15090_1002.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci CLAR306263:GH7X-1017-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence and analysis of the human pathogen Campylobacter lari."
    Miller W.G., Wang G., Binnewies T.T., Parker C.T.
    Foodborne Pathog. Dis. 5:371-386(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RM2100 / D67 / ATCC BAA-1060.

Entry informationi

Entry nameiNAPA_CAMLR
AccessioniPrimary (citable) accession number: B9KCQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3