B9KCQ2 (NAPA_CAMLR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase
EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	Cla_1018

Organism

Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) [Complete proteome] [HAMAP]

Taxonomic identifier

306263 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter › Campylobacter lari

Protein attributes

Sequence length	924 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. Binds 1 molybdenum ion per subunit By similarity. Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with NapB By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Tat-type signal Potential
	Chain	30 – 924	895	Periplasmic nitrate reductase HAMAP-Rule MF_01630		PRO_1000186351
Sites
	Metal binding	42	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	45	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	49	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	77	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9KCQ2 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 9CC5CCEFF076637A	FASTA	924	104,803
	10 20 30 40 50 60 MNRRDFIKNT AIASACGVAG LSVPSSVLAN TENKWRWDKA VCRFCGTGCG ILVARQDGKI 70 80 90 100 110 120 VAVKGDPAAP VNRGLNCIKG YFNAKIMYGE DRLVTPLLRV NAKGEFDKNG KFQQVSWQRA 130 140 150 160 170 180 FDEMEKHFKK AYKEKGVEGI GIFASGQYTI QEGYAAAKLV KAGFRSNNID PNARHCMASA 190 200 210 220 230 240 VVGFMQTFGV DEPSGCYDDI ELTDTIITWG ANMAEMHPIL WSRVSDRKLS NLDKVKIVNL 250 260 270 280 290 300 STFSNRTSHI ADTEIIFKPN TDLAIWNYIA REIVYNHPEA MDKEFVEKHC IFTTGYADIG 310 320 330 340 350 360 YGMRNNPNHP KFKASEKDTV AKQNSIILDD EEAVSLAYLG VKAGDKFEMK HQSAPDAHWE 370 380 390 400 410 420 ISFEDFKKAL EPYTLDYVAK VAKGNEDESI EEFKKKLQEL ANLYIEKNRK VVSFWTMGFN 430 440 450 460 470 480 QHTRGSWVNE QAYMVHFLLG KQAKPGSGAF SLTGQPSACG TAREVGTFSH RLPADMVVAN 490 500 510 520 530 540 PKHREISEKI WKVPAKTINP KPGAPYLKIM RDLEDGNIKF AWVQVNNPWQ NTANANHWIA 550 560 570 580 590 600 AAREMDNFIV VSDCYPGISA KVADLILPSA MIYEKWGAYG NAERRTQHWK QQVLPVGEAM 610 620 630 640 650 660 SDTWQILEFA KRFKLKEVWG ETKVDDKLTL PSILEEAKAM GYNEDDTLYD VLFANKEAKS 670 680 690 700 710 720 FKANDPIAKG FDNSDVNGDE RKIIGSDGKE FDGYGFFVQK YLWEEYRKFG LGHGHDLADF 730 740 750 760 770 780 DTYHKVRGLR WPVVNGKETQ WRFNTKFDYY AKKAAPNSDF AFYGAFAKEL PKGDLIAPQT 790 800 810 820 830 840 QEKYSLKNKA KIFFRPFMKA PERPSEEYPF WLCTGRVLEH WHSGTMTMRV PELFRAVPEA 850 860 870 880 890 900 LCYMNEDDAK KMQIAQGDIV WVESRRGKVK ARVDFRGRNK PSKGLVYVPW FDENVYINKV 910 920 TLDATCPLSN QTDFKKCAVK ITKA

References

[1]

"The complete genome sequence and analysis of the human pathogen Campylobacter lari." Miller W.G., Wang G., Binnewies T.T., Parker C.T. Foodborne Pathog. Dis. 5:371-386(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RM2100 / D67 / ATCC BAA-1060.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000932 Genomic DNA. Translation: ACM64341.1.
RefSeq	YP_002575592.1. NC_012039.1.
3D structure databases
ProteinModelPortal	B9KCQ2.
SMR	B9KCQ2. Positions 35-923.
ModBase	Search...
Protein-protein interaction databases
STRING	306263.Cla_1018.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACM64341; ACM64341; Cla_1018.
GeneID	7410753.
KEGG	cla:Cla_1018.
PATRIC	20062515. VBICamLar15090_1002.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0243.
HOGENOM	HOG000031441.
KO	K02567.
OMA	HWIKAAR.
ProtClustDB	PRK13532.
Enzyme and pathway databases
BioCyc	CLAR306263:GH7X-1060-MONOMER.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
HAMAP	MF_01630. Nitrate_reduct.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. Periplasm_NO3_reductase_lsu. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view]
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_CAMLR

Accession

Primary (citable) accession number: B9KCQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program