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B9KBR9 (BIOB_THENN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:CTN_0289
OrganismThermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E) [Complete proteome] [HAMAP]
Taxonomic identifier309803 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Biotin synthase HAMAP-Rule MF_01694
PRO_0000381684

Sites

Metal binding201Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding241Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding271Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding961Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1561Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2271Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B9KBR9 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: A413939C312B7083

FASTA27931,029
        10         20         30         40         50         60 
MVRNSRLDIC SIVNAKSGIC DQDCKFCAQS SLYNTGVKRY PLLDEESILE KAKEAEKMGA 

        70         80         90        100        110        120 
VRFGIVTSGK RPTRDELLKI ASIIEFLKVT TSLRICASLG TLEKDELSYL KDHGLDRYHH 

       130        140        150        160        170        180 
NLETSPGFFQ NICTTHTFYD RVKTVENAKS IGLEVCSGGI FGVGESFEDR LELARILKDL 

       190        200        210        220        230        240 
EVDSVPINFL IPIKGTPFEN FPELDVVERI KTIAAFRIVL GENVTIKIAA GREKLGDFQA 

       250        260        270 
LAFFAGANGM IVGGYLTVKG RSYDDDRKLV EGLKELMGW 

« Hide

References

[1]"The genome sequence of the hyperthermophilic bacterium Thermotoga neapolitana."
Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J., Kim J.J., Park K.J., Lee S.Y.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49049 / DSM 4359 / NS-E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000916 Genomic DNA. Translation: ACM22464.1.
RefSeqYP_002533831.1. NC_011978.1.

3D structure databases

ProteinModelPortalB9KBR9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING309803.CTN_0289.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM22464; ACM22464; CTN_0289.
GeneID7379165.
KEGGtna:CTN_0289.
PATRIC23939334. VBITheNea118396_0283.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycTNEA309803:GJFG-300-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_THENN
AccessionPrimary (citable) accession number: B9KBR9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways