SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B9K8X3

- SYI_THENN

UniProt

B9K8X3 - SYI_THENN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, CTN_1230
Organism
Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylate By similarity
Binding sitei596 – 5961ATP By similarity
Metal bindingi886 – 8861Zinc By similarity
Metal bindingi889 – 8891Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTNEA309803:GJFG-1273-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CTN_1230
OrganismiThermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Taxonomic identifieri309803 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000000445: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189209Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi309803.CTN_1230.

Structurei

3D structure databases

ProteinModelPortaliB9K8X3.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi593 – 5975"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9K8X3-1 [UniParc]FASTAAdd to Basket

« Hide

MDYKNTLNLP KTSFPMRANL VNKEKAFLKE WEEMDLYNYV LEQRKGKPLF    50
VLHDGPPYAN GHIHIGTALN KILKDIVVKY KTMRGYRAPY VPGWDTHGLP 100
IEHRVSQELG DRIKEMSPAE IRKKCEEFAL KFVEIQKEEF KRLGVRGDWN 150
NPYITLKPDY EVKILDVFKT LVEQGNVYRS LKPIYWCPRC RTALAEAEIE 200
YHDHRSPSIY VKFRSKDDPN LYIVIWTTTP WTLPANVGIA LHPDFEYSVV 250
KVGDERWVIA TDLLETFSRE TGVDCSEVVE KIRGKDLEGK EFQHPIFEDK 300
TSRVILADYV SLETGTGCVH IAPGHGEEDY VYGHVKYGLP IVSPVDEEGR 350
FTDEAGKYRG MFIEDSNRVI IEDLKEKGIL VHASTITHSY PHCWRCKGPV 400
IFRATEQWFI SVDHNNLRQR VLEEIDRVKW IPEWGRNRIR SMVEERPDWC 450
ISRQRVWGTP IPAVKCKECG EVTLDPKVIE HFMKIVEKEG TNAWFEKDVE 500
ELIPDDFRCP KCGARSFEKM LDTLDVWIDS GSSFEYITTR EDHPFPLDMY 550
LEGSDQHRGW FHSSIFLAVA KRGSAPYKEV LTHGFIKDEL GRKMSKSLGN 600
VVDPMEVVEK YGAEILRLWL ASSDYFNDIK ISMRIVEQQT EVYKKIRNTF 650
RFLLGNLEDF DPELDRVPYE KLLTIDKWAL GRLQEIIKRA TEYYDSYEFS 700
KVYNLVVKYC TTELSSLYLD VVKDRLYVEA KDSLYRRSAQ TVMHEILIAL 750
MKILAPIMTF TMEEVYSHLH EKDRKYKTVQ AEYWPEYRED LIDKKIMEDF 800
EKLLSIREDV LKALEEKRQQ DVIGHSLDAE VILVPRNDSV KALLEEYRDV 850
LEELFIVSKV SLSDGSGELK GELVEVTAKH AEGEKCQRCW KYTTEISRSE 900
EFPAVCPRCL AVLKGERK 918
Length:918
Mass (Da):107,092
Last modified:March 24, 2009 - v1
Checksum:iB32E1C1536F43AC6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000916 Genomic DNA. Translation: ACM23406.1.
RefSeqiWP_015919721.1. NC_011978.1.
YP_002534772.1. NC_011978.1.

Genome annotation databases

EnsemblBacteriaiACM23406; ACM23406; CTN_1230.
GeneIDi7379121.
KEGGitna:CTN_1230.
PATRICi23941268. VBITheNea118396_1217.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000916 Genomic DNA. Translation: ACM23406.1 .
RefSeqi WP_015919721.1. NC_011978.1.
YP_002534772.1. NC_011978.1.

3D structure databases

ProteinModelPortali B9K8X3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 309803.CTN_1230.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM23406 ; ACM23406 ; CTN_1230 .
GeneIDi 7379121.
KEGGi tna:CTN_1230.
PATRICi 23941268. VBITheNea118396_1217.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TNEA309803:GJFG-1273-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome sequence of the hyperthermophilic bacterium Thermotoga neapolitana."
    Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J., Kim J.J., Park K.J., Lee S.Y.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49049 / DSM 4359 / NS-E.

Entry informationi

Entry nameiSYI_THENN
AccessioniPrimary (citable) accession number: B9K8X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi