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B9K8X3

- SYI_THENN

UniProt

B9K8X3 - SYI_THENN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
    Binding sitei596 – 5961ATPUniRule annotation
    Metal bindingi886 – 8861ZincUniRule annotation
    Metal bindingi889 – 8891ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi909 – 9091ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciTNEA309803:GJFG-1273-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CTN_1230
    OrganismiThermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
    Taxonomic identifieri309803 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000000445: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 918918Isoleucine--tRNA ligasePRO_1000189209Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi309803.CTN_1230.

    Structurei

    3D structure databases

    ProteinModelPortaliB9K8X3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9K8X3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKNTLNLP KTSFPMRANL VNKEKAFLKE WEEMDLYNYV LEQRKGKPLF    50
    VLHDGPPYAN GHIHIGTALN KILKDIVVKY KTMRGYRAPY VPGWDTHGLP 100
    IEHRVSQELG DRIKEMSPAE IRKKCEEFAL KFVEIQKEEF KRLGVRGDWN 150
    NPYITLKPDY EVKILDVFKT LVEQGNVYRS LKPIYWCPRC RTALAEAEIE 200
    YHDHRSPSIY VKFRSKDDPN LYIVIWTTTP WTLPANVGIA LHPDFEYSVV 250
    KVGDERWVIA TDLLETFSRE TGVDCSEVVE KIRGKDLEGK EFQHPIFEDK 300
    TSRVILADYV SLETGTGCVH IAPGHGEEDY VYGHVKYGLP IVSPVDEEGR 350
    FTDEAGKYRG MFIEDSNRVI IEDLKEKGIL VHASTITHSY PHCWRCKGPV 400
    IFRATEQWFI SVDHNNLRQR VLEEIDRVKW IPEWGRNRIR SMVEERPDWC 450
    ISRQRVWGTP IPAVKCKECG EVTLDPKVIE HFMKIVEKEG TNAWFEKDVE 500
    ELIPDDFRCP KCGARSFEKM LDTLDVWIDS GSSFEYITTR EDHPFPLDMY 550
    LEGSDQHRGW FHSSIFLAVA KRGSAPYKEV LTHGFIKDEL GRKMSKSLGN 600
    VVDPMEVVEK YGAEILRLWL ASSDYFNDIK ISMRIVEQQT EVYKKIRNTF 650
    RFLLGNLEDF DPELDRVPYE KLLTIDKWAL GRLQEIIKRA TEYYDSYEFS 700
    KVYNLVVKYC TTELSSLYLD VVKDRLYVEA KDSLYRRSAQ TVMHEILIAL 750
    MKILAPIMTF TMEEVYSHLH EKDRKYKTVQ AEYWPEYRED LIDKKIMEDF 800
    EKLLSIREDV LKALEEKRQQ DVIGHSLDAE VILVPRNDSV KALLEEYRDV 850
    LEELFIVSKV SLSDGSGELK GELVEVTAKH AEGEKCQRCW KYTTEISRSE 900
    EFPAVCPRCL AVLKGERK 918
    Length:918
    Mass (Da):107,092
    Last modified:March 24, 2009 - v1
    Checksum:iB32E1C1536F43AC6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000916 Genomic DNA. Translation: ACM23406.1.
    RefSeqiWP_015919721.1. NC_011978.1.
    YP_002534772.1. NC_011978.1.

    Genome annotation databases

    EnsemblBacteriaiACM23406; ACM23406; CTN_1230.
    GeneIDi7379121.
    KEGGitna:CTN_1230.
    PATRICi23941268. VBITheNea118396_1217.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000916 Genomic DNA. Translation: ACM23406.1 .
    RefSeqi WP_015919721.1. NC_011978.1.
    YP_002534772.1. NC_011978.1.

    3D structure databases

    ProteinModelPortali B9K8X3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 309803.CTN_1230.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM23406 ; ACM23406 ; CTN_1230 .
    GeneIDi 7379121.
    KEGGi tna:CTN_1230.
    PATRICi 23941268. VBITheNea118396_1217.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci TNEA309803:GJFG-1273-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of the hyperthermophilic bacterium Thermotoga neapolitana."
      Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J., Kim J.J., Park K.J., Lee S.Y.
      Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49049 / DSM 4359 / NS-E.

    Entry informationi

    Entry nameiSYI_THENN
    AccessioniPrimary (citable) accession number: B9K8X3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3