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B9K8X3 (SYI_THENN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:CTN_1230
OrganismThermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E) [Complete proteome] [HAMAP]
Taxonomic identifier309803 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length918 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 918918Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_1000189209

Regions

Motif57 – 6711"HIGH" region HAMAP-Rule MF_02002
Motif593 – 5975"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8861Zinc By similarity
Metal binding8891Zinc By similarity
Metal binding9061Zinc By similarity
Metal binding9091Zinc By similarity
Binding site5521Aminoacyl-adenylate By similarity
Binding site5961ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B9K8X3 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: B32E1C1536F43AC6

FASTA918107,092
        10         20         30         40         50         60 
MDYKNTLNLP KTSFPMRANL VNKEKAFLKE WEEMDLYNYV LEQRKGKPLF VLHDGPPYAN 

        70         80         90        100        110        120 
GHIHIGTALN KILKDIVVKY KTMRGYRAPY VPGWDTHGLP IEHRVSQELG DRIKEMSPAE 

       130        140        150        160        170        180 
IRKKCEEFAL KFVEIQKEEF KRLGVRGDWN NPYITLKPDY EVKILDVFKT LVEQGNVYRS 

       190        200        210        220        230        240 
LKPIYWCPRC RTALAEAEIE YHDHRSPSIY VKFRSKDDPN LYIVIWTTTP WTLPANVGIA 

       250        260        270        280        290        300 
LHPDFEYSVV KVGDERWVIA TDLLETFSRE TGVDCSEVVE KIRGKDLEGK EFQHPIFEDK 

       310        320        330        340        350        360 
TSRVILADYV SLETGTGCVH IAPGHGEEDY VYGHVKYGLP IVSPVDEEGR FTDEAGKYRG 

       370        380        390        400        410        420 
MFIEDSNRVI IEDLKEKGIL VHASTITHSY PHCWRCKGPV IFRATEQWFI SVDHNNLRQR 

       430        440        450        460        470        480 
VLEEIDRVKW IPEWGRNRIR SMVEERPDWC ISRQRVWGTP IPAVKCKECG EVTLDPKVIE 

       490        500        510        520        530        540 
HFMKIVEKEG TNAWFEKDVE ELIPDDFRCP KCGARSFEKM LDTLDVWIDS GSSFEYITTR 

       550        560        570        580        590        600 
EDHPFPLDMY LEGSDQHRGW FHSSIFLAVA KRGSAPYKEV LTHGFIKDEL GRKMSKSLGN 

       610        620        630        640        650        660 
VVDPMEVVEK YGAEILRLWL ASSDYFNDIK ISMRIVEQQT EVYKKIRNTF RFLLGNLEDF 

       670        680        690        700        710        720 
DPELDRVPYE KLLTIDKWAL GRLQEIIKRA TEYYDSYEFS KVYNLVVKYC TTELSSLYLD 

       730        740        750        760        770        780 
VVKDRLYVEA KDSLYRRSAQ TVMHEILIAL MKILAPIMTF TMEEVYSHLH EKDRKYKTVQ 

       790        800        810        820        830        840 
AEYWPEYRED LIDKKIMEDF EKLLSIREDV LKALEEKRQQ DVIGHSLDAE VILVPRNDSV 

       850        860        870        880        890        900 
KALLEEYRDV LEELFIVSKV SLSDGSGELK GELVEVTAKH AEGEKCQRCW KYTTEISRSE 

       910 
EFPAVCPRCL AVLKGERK 

« Hide

References

[1]"The genome sequence of the hyperthermophilic bacterium Thermotoga neapolitana."
Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J., Kim J.J., Park K.J., Lee S.Y.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49049 / DSM 4359 / NS-E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000916 Genomic DNA. Translation: ACM23406.1.
RefSeqYP_002534772.1. NC_011978.1.

3D structure databases

ProteinModelPortalB9K8X3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING309803.CTN_1230.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM23406; ACM23406; CTN_1230.
GeneID7379121.
KEGGtna:CTN_1230.
PATRIC23941268. VBITheNea118396_1217.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OrthoDBEOG644ZM1.

Enzyme and pathway databases

BioCycTNEA309803:GJFG-1273-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_THENN
AccessionPrimary (citable) accession number: B9K8X3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries