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B9K8S7 (ASSY_THENN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:CTN_1184
OrganismThermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E) [Complete proteome] [HAMAP]
Taxonomic identifier309803 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 409409Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_1000116294

Regions

Nucleotide binding8 – 169ATP By similarity

Sites

Binding site341ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site851Citrulline By similarity
Binding site1151ATP; via amide nitrogen By similarity
Binding site1171Aspartate By similarity
Binding site1211Aspartate By similarity
Binding site1211Citrulline By similarity
Binding site1221Aspartate By similarity
Binding site1251Citrulline By similarity
Binding site1781Citrulline By similarity
Binding site1871Citrulline By similarity
Binding site2681Citrulline By similarity
Binding site2801Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
B9K8S7 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 3A98E4F518E3B1D1

FASTA40946,234
        10         20         30         40         50         60 
MKEKVVLAYS GGLDTSVILK WLCEKGFEVI AYVANVGQKD DFEAIKEKAL KTGASKVYIE 

        70         80         90        100        110        120 
DLRREFVTDY IFTALLGNAL YEGRYLLGTA IARPLIAKRQ VEIAEKEGAQ YVAHGATGKG 

       130        140        150        160        170        180 
NDQVRFELTY AALNPNLKVI SPWKDPEFLS RFKGRTDLIN YAREKGIPVK VSKKRPYSED 

       190        200        210        220        230        240 
ENLMHISHEA GKLEDPMYIP DEDVFTWTVS PKEAPDEETL LEIHFEKGVP VKVVNLKDGT 

       250        260        270        280        290        300 
EKTDPLELFE YLNEIGAKNG VGRLDMVENR FIGIKSRGVY ETPGATILWI AHRDLEGITM 

       310        320        330        340        350        360 
DKEVMHLRDM LAPKFSELIY NGFWFSPEME FLLAAFKKSQ ENVTGKVIVS VYKGNVMPVA 

       370        380        390        400 
RYSPCSLYNP ELSSMDVEGG FDATDSKGFI NIHALRLKVY RLTKKGYEK 

« Hide

References

[1]"The genome sequence of the hyperthermophilic bacterium Thermotoga neapolitana."
Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J., Kim J.J., Park K.J., Lee S.Y.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49049 / DSM 4359 / NS-E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000916 Genomic DNA. Translation: ACM23360.1.
RefSeqYP_002534726.1. NC_011978.1.

3D structure databases

ProteinModelPortalB9K8S7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING309803.CTN_1184.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM23360; ACM23360; CTN_1184.
GeneID7379075.
KEGGtna:CTN_1184.
PATRIC23941172. VBITheNea118396_1172.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OrthoDBEOG6K9QCV.

Enzyme and pathway databases

BioCycTNEA309803:GJFG-1223-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_THENN
AccessionPrimary (citable) accession number: B9K8S7
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways