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Protein

Argininosuccinate synthase

Gene

argG

Organism
Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei85 – 851CitrullineUniRule annotation
Binding sitei115 – 1151ATP; via amide nitrogenUniRule annotation
Binding sitei117 – 1171AspartateUniRule annotation
Binding sitei121 – 1211AspartateUniRule annotation
Binding sitei121 – 1211CitrullineUniRule annotation
Binding sitei122 – 1221AspartateUniRule annotation
Binding sitei125 – 1251CitrullineUniRule annotation
Binding sitei178 – 1781CitrullineUniRule annotation
Binding sitei187 – 1871CitrullineUniRule annotation
Binding sitei268 – 2681CitrullineUniRule annotation
Binding sitei280 – 2801CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 169ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTNEA309803:GJFG-1223-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:CTN_1184
OrganismiThermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Taxonomic identifieri309803 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000000445 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409Argininosuccinate synthasePRO_1000116294Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi309803.CTN_1184.

Structurei

3D structure databases

ProteinModelPortaliB9K8S7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9K8S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEKVVLAYS GGLDTSVILK WLCEKGFEVI AYVANVGQKD DFEAIKEKAL
60 70 80 90 100
KTGASKVYIE DLRREFVTDY IFTALLGNAL YEGRYLLGTA IARPLIAKRQ
110 120 130 140 150
VEIAEKEGAQ YVAHGATGKG NDQVRFELTY AALNPNLKVI SPWKDPEFLS
160 170 180 190 200
RFKGRTDLIN YAREKGIPVK VSKKRPYSED ENLMHISHEA GKLEDPMYIP
210 220 230 240 250
DEDVFTWTVS PKEAPDEETL LEIHFEKGVP VKVVNLKDGT EKTDPLELFE
260 270 280 290 300
YLNEIGAKNG VGRLDMVENR FIGIKSRGVY ETPGATILWI AHRDLEGITM
310 320 330 340 350
DKEVMHLRDM LAPKFSELIY NGFWFSPEME FLLAAFKKSQ ENVTGKVIVS
360 370 380 390 400
VYKGNVMPVA RYSPCSLYNP ELSSMDVEGG FDATDSKGFI NIHALRLKVY

RLTKKGYEK
Length:409
Mass (Da):46,234
Last modified:March 24, 2009 - v1
Checksum:i3A98E4F518E3B1D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000916 Genomic DNA. Translation: ACM23360.1.
RefSeqiWP_015919675.1. NC_011978.1.

Genome annotation databases

EnsemblBacteriaiACM23360; ACM23360; CTN_1184.
KEGGitna:CTN_1184.
PATRICi23941172. VBITheNea118396_1172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000916 Genomic DNA. Translation: ACM23360.1.
RefSeqiWP_015919675.1. NC_011978.1.

3D structure databases

ProteinModelPortaliB9K8S7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi309803.CTN_1184.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM23360; ACM23360; CTN_1184.
KEGGitna:CTN_1184.
PATRICi23941172. VBITheNea118396_1172.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciTNEA309803:GJFG-1223-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the hyperthermophilic bacterium Thermotoga neapolitana."
    Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J., Kim J.J., Park K.J., Lee S.Y.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49049 / DSM 4359 / NS-E.

Entry informationi

Entry nameiASSY_THENN
AccessioniPrimary (citable) accession number: B9K8S7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: July 22, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.