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B9K7M5 (BGLA_THENN) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase A

EC=3.2.1.21
Alternative name(s):
Beta-D-glucoside glucohydrolase
Cellobiase
Gentiobiase
Gene names
Name:bglA
Synonyms:gghA
Ordered Locus Names:CTN_0782
OrganismThermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E) [Complete proteome] [HAMAP]
Taxonomic identifier309803 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme has broad substrate specificity. Acts more readily on cellotetraose than on cellobiose (16-fold greater Kcat/Km).

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Pathway

Glycan metabolism; cellulose degradation.

Subunit structure

Monomer.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Biophysicochemical properties

Kinetic parameters:

KM=28.6 mM for cellobiose

KM=4.55 mM for cellotriose

KM=2.15 mM for cellotetraose

pH dependence:

Optimum pH is 6.5.

Temperature dependence:

Optimum temperature is 95 degrees Celsius.

Sequence caution

The sequence ACM22958.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Beta-glucosidase A
PRO_0000372089

Sites

Active site1641Proton donor Potential
Active site3491Nucleophile By similarity

Experimental info

Sequence conflict4361V → G in AAB95492. Ref.1

Sequences

Sequence LengthMass (Da)Tools
B9K7M5 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 56A2D49A20A8CE01

FASTA44451,535
        10         20         30         40         50         60 
MKKFPEGFLW GVATASYQIE GSPLADGAGM SIWHTFSHTP GNVKNGDTGD VACDHYNRWK 

        70         80         90        100        110        120 
EDIEIIEKIG AKAYRFSISW PRILPEGTGK VNQKGLDFYN RIIDTLLEKN ITPFITIYHW 

       130        140        150        160        170        180 
DLPFSLQLKG GWANRDIADW FAEYSRVLFE NFGDRVKHWI TLNEPWVVAI VGHLYGVHAP 

       190        200        210        220        230        240 
GMKDIYVAFH TVHNLLRAHA KSVKVFRETV KDGKIGIVFN NGYFEPASER EEDIRAARFM 

       250        260        270        280        290        300 
HQFNNYPLFL NPIYRGEYPD LVLEFAREYL PRNYEDDMEE IKQEIDFVGL NYYSGHMVKY 

       310        320        330        340        350        360 
DPNSPARVSF VERNLPKTAM GWEIVPEGIY WILKGVKEEY NPQEVYITEN GAAFDDVVSE 

       370        380        390        400        410        420 
GGKVHDQNRI DYLRAHIEQV WRAIQDGVPL KGYFVWSLLD NFEWAEGYSK RFGIVYVDYN 

       430        440 
TQKRIIKDSG YWYSNVIKNN GLTD 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana."
Yernool D.A., McCarthy J.K., Eveleigh D.E., Bok J.-D.
J. Bacteriol. 182:5172-5179(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, CHARACTERIZATION.
[2]"The genome sequence of the hyperthermophilic bacterium Thermotoga neapolitana."
Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J., Kim J.J., Park K.J., Lee S.Y.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49049 / DSM 4359 / NS-E.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF039487 Genomic DNA. Translation: AAB95492.2.
CP000916 Genomic DNA. Translation: ACM22958.1. Different initiation.
RefSeqYP_002534324.1. NC_011978.1.

3D structure databases

ProteinModelPortalB9K7M5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING309803.CTN_0782.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM22958; ACM22958; CTN_0782.
GeneID7378641.
KEGGtna:CTN_0782.
PATRIC23940329. VBITheNea118396_0765.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
KOK05350.
OrthoDBEOG60658J.
ProtClustDBCLSK971401.

Enzyme and pathway databases

BioCycTNEA309803:GJFG-809-MONOMER.
UniPathwayUPA00696.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR03356. BGL. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGLA_THENN
AccessionPrimary (citable) accession number: B9K7M5
Secondary accession number(s): O33843, O52505
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: November 13, 2013
This is version 35 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries