B9K7M5 (BGLA_THENN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-glucosidase A
EC=3.2.1.21

Alternative name(s):
Beta-D-glucoside glucohydrolase
Cellobiase
Gentiobiase

Gene names

Name:	bglA
Synonyms:	gghA
Ordered Locus Names:	CTN_0782

Organism

Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E) [Complete proteome] [HAMAP]

Taxonomic identifier

309803 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme has broad substrate specificity. Acts more readily on cellotetraose than on cellobiose (16-fold greater Kcat/Km).
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Pathway	Glycan metabolism; cellulose degradation.
Subunit structure	Monomer.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Biophysicochemical properties	Kinetic parameters: KM=28.6 mM for cellobiose KM=4.55 mM for cellotriose KM=2.15 mM for cellotetraose pH dependence: Optimum pH is 6.5. Temperature dependence: Optimum temperature is 95 degrees Celsius.
Sequence caution	The sequence ACM22958.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Beta-glucosidase A		PRO_0000372089
Sites
	Active site	164	1	Proton donor Potential
	Active site	349	1	Nucleophile By similarity
Experimental info
	Sequence conflict	436	1	V → G in AAB95492. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	B9K7M5 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 56A2D49A20A8CE01	FASTA	444	51,535
	10 20 30 40 50 60 MKKFPEGFLW GVATASYQIE GSPLADGAGM SIWHTFSHTP GNVKNGDTGD VACDHYNRWK 70 80 90 100 110 120 EDIEIIEKIG AKAYRFSISW PRILPEGTGK VNQKGLDFYN RIIDTLLEKN ITPFITIYHW 130 140 150 160 170 180 DLPFSLQLKG GWANRDIADW FAEYSRVLFE NFGDRVKHWI TLNEPWVVAI VGHLYGVHAP 190 200 210 220 230 240 GMKDIYVAFH TVHNLLRAHA KSVKVFRETV KDGKIGIVFN NGYFEPASER EEDIRAARFM 250 260 270 280 290 300 HQFNNYPLFL NPIYRGEYPD LVLEFAREYL PRNYEDDMEE IKQEIDFVGL NYYSGHMVKY 310 320 330 340 350 360 DPNSPARVSF VERNLPKTAM GWEIVPEGIY WILKGVKEEY NPQEVYITEN GAAFDDVVSE 370 380 390 400 410 420 GGKVHDQNRI DYLRAHIEQV WRAIQDGVPL KGYFVWSLLD NFEWAEGYSK RFGIVYVDYN 430 440 TQKRIIKDSG YWYSNVIKNN GLTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana." Yernool D.A., McCarthy J.K., Eveleigh D.E., Bok J.-D. J. Bacteriol. 182:5172-5179(2000) [PubMed: 10960102] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, CHARACTERIZATION.
[2]	"The genome sequence of the hyperthermophilic bacterium Thermotoga neapolitana." Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J., Kim J.J., Park K.J., Lee S.Y. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49049 / DSM 4359 / NS-E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF039487 Genomic DNA. Translation: AAB95492.2. CP000916 Genomic DNA. Translation: ACM22958.1. Different initiation.
RefSeq	YP_002534324.1. NC_011978.1.
3D structure databases
ProteinModelPortal	B9K7M5.
ModBase	Search...
Protein-protein interaction databases
STRING	B9K7M5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7378641.
GenomeReviews	Gene locus CTN_0782 in contig CP000916_GR.
KEGG	tna:CTN_0782.
PATRIC	23940329. VBITheNea118396_0765.
Organism-specific databases
CMR	Search...
Phylogenomic databases
ProtClustDB	CLSK971401.
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR017736. Glyco_hydro_1_beta-glucosidase. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K05350.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR03356. BGL. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLA_THENN

Accession

Primary (citable) accession number: B9K7M5
Secondary accession number(s): O33843, O52505

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	January 25, 2012
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program