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B9K7M5

- BGLA_THENN

UniProt

B9K7M5 - BGLA_THENN

Protein

Beta-glucosidase A

Gene

bglA

Organism
Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 37 (01 Oct 2014)
      Sequence version 2 (05 May 2009)
      Previous versions | rss
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    • Comment

    Functioni

    This enzyme has broad substrate specificity. Acts more readily on cellotetraose than on cellobiose (16-fold greater Kcat/Km).

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Kineticsi

    1. KM=28.6 mM for cellobiose
    2. KM=4.55 mM for cellotriose
    3. KM=2.15 mM for cellotetraose

    pH dependencei

    Optimum pH is 6.5.

    Temperature dependencei

    Optimum temperature is 95 degrees Celsius.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 1641Proton donorSequence Analysis
    Active sitei349 – 3491NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciTNEA309803:GJFG-809-MONOMER.
    UniPathwayiUPA00696.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase A (EC:3.2.1.21)
    Alternative name(s):
    Beta-D-glucoside glucohydrolase
    Cellobiase
    Gentiobiase
    Gene namesi
    Name:bglA
    Synonyms:gghA
    Ordered Locus Names:CTN_0782
    OrganismiThermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
    Taxonomic identifieri309803 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000000445: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 444444Beta-glucosidase APRO_0000372089Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi309803.CTN_0782.

    Structurei

    3D structure databases

    ProteinModelPortaliB9K7M5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    KOiK05350.
    OrthoDBiEOG60658J.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR017736. Glyco_hydro_1_beta-glucosidase.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    TIGRFAMsiTIGR03356. BGL. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9K7M5-1 [UniParc]FASTAAdd to Basket

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    MKKFPEGFLW GVATASYQIE GSPLADGAGM SIWHTFSHTP GNVKNGDTGD    50
    VACDHYNRWK EDIEIIEKIG AKAYRFSISW PRILPEGTGK VNQKGLDFYN 100
    RIIDTLLEKN ITPFITIYHW DLPFSLQLKG GWANRDIADW FAEYSRVLFE 150
    NFGDRVKHWI TLNEPWVVAI VGHLYGVHAP GMKDIYVAFH TVHNLLRAHA 200
    KSVKVFRETV KDGKIGIVFN NGYFEPASER EEDIRAARFM HQFNNYPLFL 250
    NPIYRGEYPD LVLEFAREYL PRNYEDDMEE IKQEIDFVGL NYYSGHMVKY 300
    DPNSPARVSF VERNLPKTAM GWEIVPEGIY WILKGVKEEY NPQEVYITEN 350
    GAAFDDVVSE GGKVHDQNRI DYLRAHIEQV WRAIQDGVPL KGYFVWSLLD 400
    NFEWAEGYSK RFGIVYVDYN TQKRIIKDSG YWYSNVIKNN GLTD 444
    Length:444
    Mass (Da):51,535
    Last modified:May 5, 2009 - v2
    Checksum:i56A2D49A20A8CE01
    GO

    Sequence cautioni

    The sequence ACM22958.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti436 – 4361V → G in AAB95492. (PubMed:10960102)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039487 Genomic DNA. Translation: AAB95492.2.
    CP000916 Genomic DNA. Translation: ACM22958.1. Different initiation.
    RefSeqiYP_002534324.1. NC_011978.1.

    Genome annotation databases

    EnsemblBacteriaiACM22958; ACM22958; CTN_0782.
    GeneIDi7378641.
    KEGGitna:CTN_0782.
    PATRICi23940329. VBITheNea118396_0765.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039487 Genomic DNA. Translation: AAB95492.2 .
    CP000916 Genomic DNA. Translation: ACM22958.1 . Different initiation.
    RefSeqi YP_002534324.1. NC_011978.1.

    3D structure databases

    ProteinModelPortali B9K7M5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 309803.CTN_0782.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM22958 ; ACM22958 ; CTN_0782 .
    GeneIDi 7378641.
    KEGGi tna:CTN_0782.
    PATRICi 23940329. VBITheNea118396_0765.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    KOi K05350.
    OrthoDBi EOG60658J.

    Enzyme and pathway databases

    UniPathwayi UPA00696 .
    BioCyci TNEA309803:GJFG-809-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR017736. Glyco_hydro_1_beta-glucosidase.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    TIGRFAMsi TIGR03356. BGL. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana."
      Yernool D.A., McCarthy J.K., Eveleigh D.E., Bok J.-D.
      J. Bacteriol. 182:5172-5179(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, CHARACTERIZATION.
    2. "The genome sequence of the hyperthermophilic bacterium Thermotoga neapolitana."
      Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J., Kim J.J., Park K.J., Lee S.Y.
      Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49049 / DSM 4359 / NS-E.

    Entry informationi

    Entry nameiBGLA_THENN
    AccessioniPrimary (citable) accession number: B9K7M5
    Secondary accession number(s): O33843, O52505
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 37 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3