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B9K7M5

- BGLA_THENN

UniProt

B9K7M5 - BGLA_THENN

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Protein

Beta-glucosidase A

Gene

bglA

Organism
Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This enzyme has broad substrate specificity. Acts more readily on cellotetraose than on cellobiose (16-fold greater Kcat/Km).

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Kineticsi

  1. KM=28.6 mM for cellobiose
  2. KM=4.55 mM for cellotriose
  3. KM=2.15 mM for cellotetraose

pH dependencei

Optimum pH is 6.5.

Temperature dependencei

Optimum temperature is 95 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641Proton donorSequence Analysis
Active sitei349 – 3491NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciTNEA309803:GJFG-809-MONOMER.
UniPathwayiUPA00696.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase A (EC:3.2.1.21)
Alternative name(s):
Beta-D-glucoside glucohydrolase
Cellobiase
Gentiobiase
Gene namesi
Name:bglA
Synonyms:gghA
Ordered Locus Names:CTN_0782
OrganismiThermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NS-E)
Taxonomic identifieri309803 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000000445: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Beta-glucosidase APRO_0000372089Add
BLAST

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi309803.CTN_0782.

Structurei

3D structure databases

ProteinModelPortaliB9K7M5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
KOiK05350.
OrthoDBiEOG60658J.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR03356. BGL. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9K7M5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKFPEGFLW GVATASYQIE GSPLADGAGM SIWHTFSHTP GNVKNGDTGD
60 70 80 90 100
VACDHYNRWK EDIEIIEKIG AKAYRFSISW PRILPEGTGK VNQKGLDFYN
110 120 130 140 150
RIIDTLLEKN ITPFITIYHW DLPFSLQLKG GWANRDIADW FAEYSRVLFE
160 170 180 190 200
NFGDRVKHWI TLNEPWVVAI VGHLYGVHAP GMKDIYVAFH TVHNLLRAHA
210 220 230 240 250
KSVKVFRETV KDGKIGIVFN NGYFEPASER EEDIRAARFM HQFNNYPLFL
260 270 280 290 300
NPIYRGEYPD LVLEFAREYL PRNYEDDMEE IKQEIDFVGL NYYSGHMVKY
310 320 330 340 350
DPNSPARVSF VERNLPKTAM GWEIVPEGIY WILKGVKEEY NPQEVYITEN
360 370 380 390 400
GAAFDDVVSE GGKVHDQNRI DYLRAHIEQV WRAIQDGVPL KGYFVWSLLD
410 420 430 440
NFEWAEGYSK RFGIVYVDYN TQKRIIKDSG YWYSNVIKNN GLTD
Length:444
Mass (Da):51,535
Last modified:May 5, 2009 - v2
Checksum:i56A2D49A20A8CE01
GO

Sequence cautioni

The sequence ACM22958.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti436 – 4361V → G in AAB95492. (PubMed:10960102)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF039487 Genomic DNA. Translation: AAB95492.2.
CP000916 Genomic DNA. Translation: ACM22958.1. Different initiation.
RefSeqiYP_002534324.1. NC_011978.1.

Genome annotation databases

EnsemblBacteriaiACM22958; ACM22958; CTN_0782.
GeneIDi7378641.
KEGGitna:CTN_0782.
PATRICi23940329. VBITheNea118396_0765.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF039487 Genomic DNA. Translation: AAB95492.2 .
CP000916 Genomic DNA. Translation: ACM22958.1 . Different initiation.
RefSeqi YP_002534324.1. NC_011978.1.

3D structure databases

ProteinModelPortali B9K7M5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 309803.CTN_0782.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM22958 ; ACM22958 ; CTN_0782 .
GeneIDi 7378641.
KEGGi tna:CTN_0782.
PATRICi 23940329. VBITheNea118396_0765.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
KOi K05350.
OrthoDBi EOG60658J.

Enzyme and pathway databases

UniPathwayi UPA00696 .
BioCyci TNEA309803:GJFG-809-MONOMER.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR017736. Glyco_hydro_1_beta-glucosidase.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
TIGRFAMsi TIGR03356. BGL. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the glucooligosaccharide catabolic pathway beta-glucan glucohydrolase and cellobiose phosphorylase in the marine hyperthermophile Thermotoga neapolitana."
    Yernool D.A., McCarthy J.K., Eveleigh D.E., Bok J.-D.
    J. Bacteriol. 182:5172-5179(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, CHARACTERIZATION.
  2. "The genome sequence of the hyperthermophilic bacterium Thermotoga neapolitana."
    Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J., Kim J.J., Park K.J., Lee S.Y.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49049 / DSM 4359 / NS-E.

Entry informationi

Entry nameiBGLA_THENN
AccessioniPrimary (citable) accession number: B9K7M5
Secondary accession number(s): O33843, O52505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: October 1, 2014
This is version 37 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3