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B9JZI2

- GLND_AGRVS

UniProt

B9JZI2 - GLND_AGRVS

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, Avi_0424
Organism
Agrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis (strain S4))
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciAVIT311402:GH2Y-338-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Avi_0424
OrganismiAgrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis (strain S4))
Taxonomic identifieri311402 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium
ProteomesiUP000001596: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 941941Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_1000132525Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi311402.Avi_0424.

Structurei

3D structure databases

ProteinModelPortaliB9JZI2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini517 – 60488HDAdd
BLAST
Domaini729 – 81082ACT 1Add
BLAST
Domaini840 – 91980ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 372372UridylyltransferaseUniRule annotationAdd
BLAST
Regioni373 – 728356Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9JZI2-1 [UniParc]FASTAAdd to Basket

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MAKHDLSDAT LPDFKALEAE CMSIVLQNGK VPETRAAILP LLKKASIDGR    50
EAALRHLTTN GSGLECAGMI SNLQDNLITL VHRMVTQAVY PTTQQEFAVA 100
AVGGYGRSTL APGSDIDLLF LLSVKAGADA RKAVEFLLYI LWDLGFKVGH 150
ATRLVEECIR LSRTDMTIRT AILETRFICG EALLVGELQK RFDAEVVEKT 200
APEFIAAKLA ERDERHRKAG DTRYLVEPNV KEGKGGLRDL HTLFWIAKYY 250
YRISDPADLV KLGVLSRQEW RMFQKSDDFL WAVRCHMHFA TGKPEERLSF 300
DLQPEIARNL GYNARPGLSE VERFMKHYFH VAKNVGDLTR IVCASLEDKQ 350
AKAAPGLTAA IGRFAHRPRR IPGTPEFIED RGRIALSGPD IFKRDPINIM 400
RFFHVADLHG LEFHPDALKA ITRCLPLIDH DFRENEEANR LFLSILTSKR 450
DPALMLTRMN EAGVLGKFIP DFGRIVSMMQ FNMYHHYTVD EHLIRSVGIL 500
AEVDQGQHAD IHPLAVKLMP NIEERTVLFV AVLLHDIAKG RQEDHSIAGA 550
KVARRLCPRL GLNDKQTELV VWLIDQHLLM SMVAQTRDLH DRKTITDFAE 600
KVQSLDRLRM LLVLTVCDIR AVGPGVWNGW KGQLLRTLYY ETELLLSGGF 650
SESPRKERAK QAAEQLAEAL SDWSQKDQKT YTKLHYQPYL LTVPLEDQVR 700
HAHFIRQADK ADQALATMVR THSFHAITEI TVLAPDHPRL LSIIAGACAA 750
AGANIADAQI FTTSDGRALD TILINREFPI DEDEMRRANT ISKMIEDVLA 800
GKKRLPEVIA TRTKGRKRNK TFTVKPHVTI SNSLSNKFTV IEIECLDRIG 850
LLAEVTAVLA DLSLDIHSAR ITTFGEKVID TFYVIDLVGQ KITNENRQGS 900
ISVRLKAVMS EQPDELREQM PSGIIAPAAT KSPAAEKKAR V 941
Length:941
Mass (Da):105,604
Last modified:March 24, 2009 - v1
Checksum:i66E7CBB0A01D8CDB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000633 Genomic DNA. Translation: ACM35294.1.
RefSeqiYP_002548298.1. NC_011989.1.

Genome annotation databases

EnsemblBacteriaiACM35294; ACM35294; Avi_0424.
GeneIDi7388439.
KEGGiavi:Avi_0424.
PATRICi20825788. VBIAgrVit37146_2575.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000633 Genomic DNA. Translation: ACM35294.1 .
RefSeqi YP_002548298.1. NC_011989.1.

3D structure databases

ProteinModelPortali B9JZI2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 311402.Avi_0424.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM35294 ; ACM35294 ; Avi_0424 .
GeneIDi 7388439.
KEGGi avi:Avi_0424.
PATRICi 20825788. VBIAgrVit37146_2575.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci AVIT311402:GH2Y-338-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: S4 / ATCC BAA-846.

Entry informationi

Entry nameiGLND_AGRVS
AccessioniPrimary (citable) accession number: B9JZI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: June 11, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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