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B9JYY6 (BIOB_AGRVS) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Avi_3108
OrganismAgrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis (strain S4)) [Complete proteome] [HAMAP]
Taxonomic identifier311402 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328Biotin synthase HAMAP-Rule MF_01694
PRO_0000381188

Sites

Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding651Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1031Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1351Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1951Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2671Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B9JYY6 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: AB2A10D36ECBDCD1

FASTA32835,435
        10         20         30         40         50         60 
MSEAAGEIRN DWSVEEIVTL HNLPLLELIG HANAVHGRHH NPNVVQKASL LSIKTGGCPE 

        70         80         90        100        110        120 
DCAYCPQSAH HREVKLTKDR LMQPETVLAL AKRAKDAGAE RFCMGAAWRQ VRDGKEFDAV 

       130        140        150        160        170        180 
LTMVRGVRDL GMEACVTLGM LEKHQAEKLA EAGLTAYNHN LDTSPEFYGE IITTRSYADR 

       190        200        210        220        230        240 
LETLSIVRSF GIDLCCGGII GMGETIRDRA SMLQVLASMR PHPESVPINA LVPVEGTPLA 

       250        260        270        280        290        300 
AMPRIDPLEL VRMVATARIV MPKSTVRLSA GRSTLNREAQ ILCLVSGANS VFYGDTLLTT 

       310        320 
PNAGIGEDEA LFAAIGALPH EAAPLAAE 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000633 Genomic DNA. Translation: ACM37232.1.
RefSeqYP_002550242.1. NC_011989.1.

3D structure databases

ProteinModelPortalB9JYY6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING311402.Avi_3108.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM37232; ACM37232; Avi_3108.
GeneID7388654.
KEGGavi:Avi_3108.
PATRIC20830142. VBIAgrVit37146_4721.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2463890.

Enzyme and pathway databases

BioCycAVIT311402:GH2Y-2328-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_AGRVS
AccessionPrimary (citable) accession number: B9JYY6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: February 19, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways