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B9JV46 (SYL_AGRVS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Avi_4381
OrganismAgrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis (strain S4)) [Complete proteome] [HAMAP]
Taxonomic identifier311402 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium

Protein attributes

Sequence length876 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 876876Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199171

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif632 – 6365"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B9JV46 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: C5CD367D9EC31F3A

FASTA87697,494
        10         20         30         40         50         60 
MSTERYNPRD AEPRWQEKWS EAKIFETDNN DPREKYYVLE MFPYPSGRIH IGHVRNYAMG 

        70         80         90        100        110        120 
DVVARYKRAR GYNVLHPMGW DAFGMPAENA AMQNKVHPKD WTYQNIASMR AQLKSMGLSL 

       130        140        150        160        170        180 
DWSREFATCD VEYYQRQQYL FLDMMEKGLV YRKQSKVNWD PVDQTVLANE QVIDGRGWRS 

       190        200        210        220        230        240 
GALVEQRELT QWFFKITDFA QDLLDALDTL DHWPEKVRLM QKNWIGRSEG LAIRWEIAAG 

       250        260        270        280        290        300 
TGPQGFTDVQ VYTTRPDTLF GASFLAIAAD HPLAKALSET SSDIAAFCDE CRRAGTSLAA 

       310        320        330        340        350        360 
LETAEKKGLD TGIKVKHPLD PAWELPVYIA NFVLMDYGTG AIFACPSGDQ RDLDFARKYG 

       370        380        390        400        410        420 
LPVVPVVMPR DGDAASFTVG DTAYDGDGVM INSRFLDGLT TQEAFDVVAG KLTAAQLGNE 

       430        440        450        460        470        480 
PVAERRVNFR LRDWGVSRQR YWGCPIPVIH CDDCGVVPVP KQDLPVKLPD DVTFDVPGNP 

       490        500        510        520        530        540 
LDRHPTWRNV SCPCCGKAAR RETDTMDTFV DSSWYYTRFT APWEDKPTDP AVANHWLPVD 

       550        560        570        580        590        600 
QYIGGIEHAI LHLLYSRFFT RAMRETGHVA VSEPFKGLFT QGMVVHETYR LGSGANGQWV 

       610        620        630        640        650        660 
APADIRVEDV DGARRAFLLS SGEEVTIGSV EKMSKSKKNV IDPDDILGSY GADTARFFVL 

       670        680        690        700        710        720 
SDSPPDRDVI WSEAGIEGSH RFVQRLWRLV SEAADVLRTS ASTPAKDGAG RAVSQAAHKT 

       730        740        750        760        770        780 
LQAVGADLDK LAFNKAVARI YELLNTLAAP LTQVASGNAD TSFVAAVRNA TEILIQIIAP 

       790        800        810        820        830        840 
MMPHLAEECW AVLGHSGMVS QADWPVFHAE LVAENEVVMP VQINGKKKAE LTIGRDADQN 

       850        860        870 
AVSQAVLDLD AVKAALQGQT PKKIIVVPQR IVNIVV 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000633 Genomic DNA. Translation: ACM38184.1.
RefSeqYP_002551196.1. NC_011989.1.

3D structure databases

ProteinModelPortalB9JV46.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING311402.Avi_4381.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM38184; ACM38184; Avi_4381.
GeneID7387955.
KEGGavi:Avi_4381.
PATRIC20832232. VBIAgrVit37146_5753.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAREKNDWE.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycAVIT311402:GH2Y-3303-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_AGRVS
AccessionPrimary (citable) accession number: B9JV46
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: April 16, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries