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B9JNG1 (NADE_AGRRK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NH(3)-dependent NAD(+) synthetase

EC=6.3.1.5
Gene names
Name:nadE
Ordered Locus Names:Arad_7634
OrganismAgrobacterium radiobacter (strain K84 / ATCC BAA-868) [Complete proteome] [HAMAP]
Taxonomic identifier311403 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacterium

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193

Sequence similarities

Belongs to the NAD synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332NH(3)-dependent NAD(+) synthetase HAMAP MF_00193
PRO_1000191492

Regions

Nucleotide binding48 – 558ATP By similarity

Sites

Active site501 By similarity

Sequences

Sequence LengthMass (Da)Tools
B9JNG1 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: BAD0835F48CF0A70

FASTA33236,627
        10         20         30         40         50         60 
MNPSPLSIHS ALSSDALRID AAAEVDRIAQ AMRDQVRNKM RRRGLVIGLS GGVDSSVCAA 

        70         80         90        100        110        120 
LAAYALGAQN VFAIFMPEND SDPESLSLGQ EVAKAFSLEG AIEDIGSALA AMGCYERRDD 

       130        140        150        160        170        180 
FIRQVEPAYG SGWSCKVVIS SPLAGEGYAL STLVLQAPDG SQTRHRMPAS VYLGIVAATN 

       190        200        210        220        230        240 
MKQRTRKQME YYHADRLNYA VLGTPNRLEY DQGFFVKNGD GAADLKPIAH LYKSQVYQIA 

       250        260        270        280        290        300 
EYLGVPEEVR RRPPTTDTWS LTQTQEEFYF ALPYDRMDLC LHALNEGLGV EETAKATQLT 

       310        320        330 
TDQVERVWLD IHSKRKATRP LHLAPLLVQP VF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000629 Genomic DNA. Translation: ACM29092.1.
RefSeqYP_002540687.1. NC_011983.1.

3D structure databases

ProteinModelPortalB9JNG1.
ModBaseSearch...

Protein-protein interaction databases

STRINGB9JNG1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7365491.
GenomeReviewsGene locus Arad_7634 in contig CP000629_GR.
KEGGara:Arad_7634.
PATRIC20796774. VBIAgrRad129173_0514.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAAHLYKSQ.
ProtClustDBPRK00876.

Family and domain databases

HAMAPMF_00193. NadE.
[Tree]
InterProIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01916.
PfamPF02540. NAD_synthase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00552. NadE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADE_AGRRK
AccessionPrimary (citable) accession number: B9JNG1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families