B9JHZ9 (HGD_AGRRK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	Arad_8212

Organism

Agrobacterium radiobacter (strain K84 / ATCC BAA-868) [Complete proteome] [HAMAP]

Taxonomic identifier

311403 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Rhizobium radiobacter

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334		PRO_1000190373
Sites
	Metal binding	349	1	Iron By similarity
	Metal binding	355	1	Iron By similarity
	Metal binding	385	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9JHZ9 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: F7E70699E7AFE391	FASTA	453	50,995
	10 20 30 40 50 60 MDQTMTQASD KEASVADKLQ YMPGFGNDFE TETLPGALPQ GQNSPQKCNY GLYAEQLSGS 70 80 90 100 110 120 PFTAPRGTNE RSWLYRIRPS VRHTRRFSNA SYPHWKSAPC LDDHSLPLGQ LRWGPMPVPD 130 140 150 160 170 180 EKLSFLDGVR TMTTAGDVLT QTGMAAHVYV FNEDMVDDYA FNADGELLIV PQVGAIRVFT 190 200 210 220 230 240 EMGIMDVEPL EIVLIPRGMM FKVLRKGDEK VWRGYICENY GAKFTLPDRG PIGANCLANP 250 260 270 280 290 300 RDFKTPVAAY EDKEAPCRVH VKWCGKFYVT EIGHSPLDVV AWHGNYAPFK YDLRTFSPVG 310 320 330 340 350 360 AILFDHPDPS IFTVLTAPTE SAGTANVDFV IFPPRWLVAE HTFRPPWYHR NIMSEFMGLI 370 380 390 400 410 420 HGQYDAKEEG FVPGGISLHN MMLPHGPDAS GFEKASNSEL KPVKLDHTMA FMFETRYPQQ 430 440 450 VTKYAAELET LQDNYLECWD GLERRFDGTP GIK

References

[1]

"Genome sequences of three Agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria." Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K., Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., Otten L., Suen G., Welch R., Almeida N.F., Arnold F., Burton O.T., Du Z., Ewing A. , Godsy E., Heisel S., Houmiel K.L., Jhaveri J., Lu J., Miller N.M., Norton S., Chen Q., Phoolcharoen W., Ohlin V., Ondrusek D., Pride N., Stricklin S.L., Sun J., Wheeler C., Wilson L., Zhu H., Wood D.W. J. Bacteriol. 191:2501-2511(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K84 / ATCC BAA-868.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000629 Genomic DNA. Translation: ACM29541.1.
RefSeq	YP_002541138.1. NC_011983.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	311403.Arad_8212.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACM29541; ACM29541; Arad_8212.
GeneID	7365865.
KEGG	ara:Arad_8212.
PATRIC	20797712. VBIAgrRad129173_0983.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PRK05341.
Enzyme and pathway databases
BioCyc	ARAD311403:GHU8-5439-MONOMER.
UniPathway	UPA00139; UER00339.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
HAMAP	MF_00334. Homogentis_dioxygen.
InterPro	IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_AGRRK

Accession

Primary (citable) accession number: B9JHZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program