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B9JG75

- MASZ_AGRRK

UniProt

B9JG75 - MASZ_AGRRK

Protein

Malate synthase G

Gene

glcB

Organism
Agrobacterium radiobacter (strain K84 / ATCC BAA-868)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei273 – 2731Acetyl-CoAUniRule annotation
    Binding sitei310 – 3101Acetyl-CoAUniRule annotation
    Active sitei337 – 3371Proton acceptorUniRule annotation
    Binding sitei337 – 3371GlyoxylateUniRule annotation
    Metal bindingi429 – 4291MagnesiumUniRule annotation
    Binding sitei429 – 4291GlyoxylateUniRule annotation
    Metal bindingi457 – 4571MagnesiumUniRule annotation
    Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei628 – 6281Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARAD311403:GHU8-54-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Arad_0071
    OrganismiAgrobacterium radiobacter (strain K84 / ATCC BAA-868)
    Taxonomic identifieri311403 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
    ProteomesiUP000001600: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 722722Malate synthase GPRO_1000147422Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei614 – 6141Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi311403.Arad_0071.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni123 – 1242Acetyl-CoA bindingUniRule annotation
    Regioni454 – 4574Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B9JG75-1 [UniParc]FASTAAdd to Basket

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    MSRIEKNGLQ IEPVLYDFLV KEALPGSGVD ADTFFAGFSA IVHDLAPKNR    50
    ELLAKRDRMQ AAIDDWYRKN GAPVDLAAYE AFLREIGYII PEGPAFTVST 100
    ANVDTEIASI AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAIPETD 150
    GAEKGKGYNP KRGEKVIAWV RDFLDAAVPL SDASWKDVAG FAVKGGVLAI 200
    ATKSGTVALT DERHFAGYLG DVAAPSHILL KNNGIHIEIV LDPSTDIGKV 250
    DPAHISDVRL ESAITTIMDC EDSIAAVDAE DKAEVYRNWL GLMKGDLTEE 300
    VAKGGKTFTR RLNPDVTYTA PDGASFELHC RSLMLIRNVG HLMTNPAILD 350
    RDGNEVPEGI MDAMITGLIA LYDVGPKGRR KNSRAGSMYV VKPKMHGPEE 400
    VAFAVEIFSR VEDVLGMPRN TIKMGIMDEE RRTTVNLKEA IRAASERVVF 450
    INTGFLDRTG DEIHTSMEAG PMIRKGDMKQ AAWIAAYENW NVDIGLECGL 500
    SGHAQIGKGM WAMPDLMAAM LEQKIAHPKA GANTAWVPSP TAATLHATHY 550
    HKVNVANVQQ TLKSRVRAKL SDILSVPVAS RPNWTPEEIQ HEIDNNCQGI 600
    LGYVVRWVDQ GVGCSKVPDI NNIGLMEDRA TLRISAQHMA NWLHHKVITE 650
    AQIVETMKRM ATVVDQQNAG DPLYIAMAGN FDGSVAFQAA LELVLKGREQ 700
    PNGYTEPVLH RRRIELKAKQ AV 722
    Length:722
    Mass (Da):78,959
    Last modified:March 24, 2009 - v1
    Checksum:i7734EFD58273291D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000628 Genomic DNA. Translation: ACM24858.1.
    RefSeqiYP_002542780.1. NC_011985.1.

    Genome annotation databases

    EnsemblBacteriaiACM24858; ACM24858; Arad_0071.
    GeneIDi7368768.
    KEGGiara:Arad_0071.
    PATRICi20800755. VBIAgrRad129173_2504.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000628 Genomic DNA. Translation: ACM24858.1 .
    RefSeqi YP_002542780.1. NC_011985.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 311403.Arad_0071.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM24858 ; ACM24858 ; Arad_0071 .
    GeneIDi 7368768.
    KEGGi ara:Arad_0071.
    PATRICi 20800755. VBIAgrRad129173_2504.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci ARAD311403:GHU8-54-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K84 / ATCC BAA-868.

    Entry informationi

    Entry nameiMASZ_AGRRK
    AccessioniPrimary (citable) accession number: B9JG75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3