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Protein

Malate synthase G

Gene

glcB

Organism
Agrobacterium radiobacter (strain K84 / ATCC BAA-868)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway:iglyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei273 – 2731Acetyl-CoAUniRule annotation
Binding sitei310 – 3101Acetyl-CoAUniRule annotation
Active sitei337 – 3371Proton acceptorUniRule annotation
Binding sitei337 – 3371GlyoxylateUniRule annotation
Metal bindingi429 – 4291MagnesiumUniRule annotation
Binding sitei429 – 4291GlyoxylateUniRule annotation
Metal bindingi457 – 4571MagnesiumUniRule annotation
Binding sitei538 – 5381Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei628 – 6281Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARAD311403:GHU8-54-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Arad_0071
OrganismiAgrobacterium radiobacter (strain K84 / ATCC BAA-868)
Taxonomic identifieri311403 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000001600 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 722722Malate synthase GPRO_1000147422Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei614 – 6141Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi311403.Arad_0071.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242Acetyl-CoA bindingUniRule annotation
Regioni454 – 4574Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

B9JG75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRIEKNGLQ IEPVLYDFLV KEALPGSGVD ADTFFAGFSA IVHDLAPKNR
60 70 80 90 100
ELLAKRDRMQ AAIDDWYRKN GAPVDLAAYE AFLREIGYII PEGPAFTVST
110 120 130 140 150
ANVDTEIASI AGPQLVVPVM NARYALNAAN ARWGSLYDAL YGTDAIPETD
160 170 180 190 200
GAEKGKGYNP KRGEKVIAWV RDFLDAAVPL SDASWKDVAG FAVKGGVLAI
210 220 230 240 250
ATKSGTVALT DERHFAGYLG DVAAPSHILL KNNGIHIEIV LDPSTDIGKV
260 270 280 290 300
DPAHISDVRL ESAITTIMDC EDSIAAVDAE DKAEVYRNWL GLMKGDLTEE
310 320 330 340 350
VAKGGKTFTR RLNPDVTYTA PDGASFELHC RSLMLIRNVG HLMTNPAILD
360 370 380 390 400
RDGNEVPEGI MDAMITGLIA LYDVGPKGRR KNSRAGSMYV VKPKMHGPEE
410 420 430 440 450
VAFAVEIFSR VEDVLGMPRN TIKMGIMDEE RRTTVNLKEA IRAASERVVF
460 470 480 490 500
INTGFLDRTG DEIHTSMEAG PMIRKGDMKQ AAWIAAYENW NVDIGLECGL
510 520 530 540 550
SGHAQIGKGM WAMPDLMAAM LEQKIAHPKA GANTAWVPSP TAATLHATHY
560 570 580 590 600
HKVNVANVQQ TLKSRVRAKL SDILSVPVAS RPNWTPEEIQ HEIDNNCQGI
610 620 630 640 650
LGYVVRWVDQ GVGCSKVPDI NNIGLMEDRA TLRISAQHMA NWLHHKVITE
660 670 680 690 700
AQIVETMKRM ATVVDQQNAG DPLYIAMAGN FDGSVAFQAA LELVLKGREQ
710 720
PNGYTEPVLH RRRIELKAKQ AV
Length:722
Mass (Da):78,959
Last modified:March 24, 2009 - v1
Checksum:i7734EFD58273291D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000628 Genomic DNA. Translation: ACM24858.1.
RefSeqiWP_007698521.1. NC_011985.1.

Genome annotation databases

EnsemblBacteriaiACM24858; ACM24858; Arad_0071.
KEGGiara:Arad_0071.
PATRICi20800755. VBIAgrRad129173_2504.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000628 Genomic DNA. Translation: ACM24858.1.
RefSeqiWP_007698521.1. NC_011985.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi311403.Arad_0071.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM24858; ACM24858; Arad_0071.
KEGGiara:Arad_0071.
PATRICi20800755. VBIAgrRad129173_2504.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciARAD311403:GHU8-54-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K84 / ATCC BAA-868.

Entry informationi

Entry nameiMASZ_AGRRK
AccessioniPrimary (citable) accession number: B9JG75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: July 22, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.