B9JFR4 (GLSA_AGRRK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase
EC=3.5.1.2

Gene names

Name:	glsA
Ordered Locus Names:	Arad_2601

Organism

Agrobacterium radiobacter (strain K84 / ATCC BAA-868) [Complete proteome] [HAMAP]

Taxonomic identifier

311403 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Rhizobium radiobacter

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glutaminase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutaminase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Glutaminase HAMAP-Rule MF_00313		PRO_1000132901
Sites
	Binding site	64	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	160	1	Substrate By similarity
	Binding site	167	1	Substrate By similarity
	Binding site	191	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	261	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9JFR4 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 28104EE6A896F9F4	FASTA	309	32,718
	10 20 30 40 50 60 MADLQAVLDS IHAELSPRLG EGKVADYIPE LAKVDPNQFG MAIATVDGNI HSVGHADVPF 70 80 90 100 110 120 SIQSISKVFM LTLALGKVGE SLWNRVGREP SGTAFNSIVQ LEREEGIPRN PFVNAGAIAV 130 140 150 160 170 180 SDVVLSGHAP REAIGELLRF VRYVADDESI TIDDKVARSE TQTGFRNFAL ANFMRAYGNI 190 200 210 220 230 240 DHPVEHVLGV YFHQCAVSMS CQQLAKAGLY LAARGTNPIT GHSVVSPKRA RRINALMLTC 250 260 270 280 290 300 GHYDGSGDFA YHVGLPGKSG VGGGILAVAP GIGSIAVWSP GLNKVGNSQL GAVALEMLAA RTGWSVFGD

References

[1]

"Genome sequences of three Agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria." Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K., Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., Otten L., Suen G., Welch R., Almeida N.F., Arnold F., Burton O.T., Du Z., Ewing A. , Godsy E., Heisel S., Houmiel K.L., Jhaveri J., Lu J., Miller N.M., Norton S., Chen Q., Phoolcharoen W., Ohlin V., Ondrusek D., Pride N., Stricklin S.L., Sun J., Wheeler C., Wilson L., Zhu H., Wood D.W. J. Bacteriol. 191:2501-2511(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K84 / ATCC BAA-868.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000628 Genomic DNA. Translation: ACM26754.1.
RefSeq	YP_002544682.1. NC_011985.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	311403.Arad_2601.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACM26754; ACM26754; Arad_2601.
GeneID	7368415.
KEGG	ara:Arad_2601.
PATRIC	20804832. VBIAgrRad129173_4499.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
OMA	EPSGNPF.
ProtClustDB	PRK00971.
Enzyme and pathway databases
BioCyc	ARAD311403:GHU8-2551-MONOMER.
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR015868. Glutaminase. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF04960. Glutaminase. 1 hit. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLSA_AGRRK

Accession

Primary (citable) accession number: B9JFR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program