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B9JEZ6

- LIPA_AGRRK

UniProt

B9JEZ6 - LIPA_AGRRK

Protein

Lipoyl synthase

Gene

lipA

Organism
Agrobacterium radiobacter (strain K84 / ATCC BAA-868)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi87 – 871Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi91 – 911Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARAD311403:GHU8-1744-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:Arad_2255
    OrganismiAgrobacterium radiobacter (strain K84 / ATCC BAA-868)
    Taxonomic identifieri311403 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
    ProteomesiUP000001600: Chromosome 1

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 323323Lipoyl synthasePRO_1000124615Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi311403.Arad_2255.

    Structurei

    3D structure databases

    ProteinModelPortaliB9JEZ6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiFERIATN.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B9JEZ6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTILDRINP DAKRVRHPEK AHRPDTEVLR KPDWIRVKAP TSKGYAETRS    50
    IVKEHKLVTV CEEAGCPNIG ECWEKKHATF MIMGEICTRA CAFCNVATGK 100
    PNALDMAEPE NIAKAVKEMG LSHVVITSVD RDDLADGGAE HFEKVIWAIR 150
    AASPMTTIEI LTPDFLKKPG ALERVVAAKP DVFNHNMETV PGNYLTVRPG 200
    ARYFHSVRLL QRVKELDPTM FTKSGIMVGL GEERNEVLQL MDDLRTADVD 250
    FLTIGQYLQP TRKHHQVMSF VTPEEFKSYE TVAYTKGFLM VASSPLTRSS 300
    HHAGDDFARL RAAREKKILL AAE 323
    Length:323
    Mass (Da):36,223
    Last modified:March 24, 2009 - v1
    Checksum:i64101E82A69A5B92
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000628 Genomic DNA. Translation: ACM26487.1.
    RefSeqiWP_012651373.1. NC_011985.1.
    YP_002544414.1. NC_011985.1.

    Genome annotation databases

    EnsemblBacteriaiACM26487; ACM26487; Arad_2255.
    GeneIDi7370658.
    KEGGiara:Arad_2255.
    PATRICi20804280. VBIAgrRad129173_4226.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000628 Genomic DNA. Translation: ACM26487.1 .
    RefSeqi WP_012651373.1. NC_011985.1.
    YP_002544414.1. NC_011985.1.

    3D structure databases

    ProteinModelPortali B9JEZ6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 311403.Arad_2255.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACM26487 ; ACM26487 ; Arad_2255 .
    GeneIDi 7370658.
    KEGGi ara:Arad_2255.
    PATRICi 20804280. VBIAgrRad129173_4226.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi FERIATN.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci ARAD311403:GHU8-1744-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K84 / ATCC BAA-868.

    Entry informationi

    Entry nameiLIPA_AGRRK
    AccessioniPrimary (citable) accession number: B9JEZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3