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B9JEZ6

- LIPA_AGRRK

UniProt

B9JEZ6 - LIPA_AGRRK

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Protein

Lipoyl synthase

Gene
lipA, Arad_2255
Organism
Agrobacterium radiobacter (strain K84 / ATCC BAA-868)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi87 – 871Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi91 – 911Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARAD311403:GHU8-1744-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Arad_2255
OrganismiAgrobacterium radiobacter (strain K84 / ATCC BAA-868)
Taxonomic identifieri311403 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000001600: Chromosome 1

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Lipoyl synthaseUniRule annotationPRO_1000124615Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi311403.Arad_2255.

Structurei

3D structure databases

ProteinModelPortaliB9JEZ6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiFERIATN.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B9JEZ6-1 [UniParc]FASTAAdd to Basket

« Hide

MVTILDRINP DAKRVRHPEK AHRPDTEVLR KPDWIRVKAP TSKGYAETRS    50
IVKEHKLVTV CEEAGCPNIG ECWEKKHATF MIMGEICTRA CAFCNVATGK 100
PNALDMAEPE NIAKAVKEMG LSHVVITSVD RDDLADGGAE HFEKVIWAIR 150
AASPMTTIEI LTPDFLKKPG ALERVVAAKP DVFNHNMETV PGNYLTVRPG 200
ARYFHSVRLL QRVKELDPTM FTKSGIMVGL GEERNEVLQL MDDLRTADVD 250
FLTIGQYLQP TRKHHQVMSF VTPEEFKSYE TVAYTKGFLM VASSPLTRSS 300
HHAGDDFARL RAAREKKILL AAE 323
Length:323
Mass (Da):36,223
Last modified:March 24, 2009 - v1
Checksum:i64101E82A69A5B92
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000628 Genomic DNA. Translation: ACM26487.1.
RefSeqiWP_012651373.1. NC_011985.1.
YP_002544414.1. NC_011985.1.

Genome annotation databases

EnsemblBacteriaiACM26487; ACM26487; Arad_2255.
GeneIDi7370658.
KEGGiara:Arad_2255.
PATRICi20804280. VBIAgrRad129173_4226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000628 Genomic DNA. Translation: ACM26487.1 .
RefSeqi WP_012651373.1. NC_011985.1.
YP_002544414.1. NC_011985.1.

3D structure databases

ProteinModelPortali B9JEZ6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 311403.Arad_2255.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM26487 ; ACM26487 ; Arad_2255 .
GeneIDi 7370658.
KEGGi ara:Arad_2255.
PATRICi 20804280. VBIAgrRad129173_4226.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi FERIATN.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci ARAD311403:GHU8-1744-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K84 / ATCC BAA-868.

Entry informationi

Entry nameiLIPA_AGRRK
AccessioniPrimary (citable) accession number: B9JEZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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