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B9JER2 (PROB_AGRRK) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:Arad_4877
OrganismAgrobacterium radiobacter (strain K84 / ATCC BAA-868) [Complete proteome] [HAMAP]
Taxonomic identifier311403 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 389389Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_1000193682

Regions

Domain281 – 35878PUA
Nucleotide binding175 – 1762ATP By similarity

Sites

Binding site161ATP By similarity
Binding site561Substrate By similarity
Binding site1431Substrate By similarity
Binding site1551Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
B9JER2 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 22DFEB955991BC6B

FASTA38940,771
        10         20         30         40         50         60 
MTARKSLDRY RRIVIKIGSA LLVDRKTGLK KAWLDGMCAD IAALKAKGVD VLVVSSGAIA 

        70         80         90        100        110        120 
LGRSVLDLPS GALKLEESQA AAAVGQISLA RAWSESLSHD DIVAGQILLT LGDTEERRRY 

       130        140        150        160        170        180 
LNARATINQL LKIGAVPIIN ENDTVATSEI RYGDNDRLAA RVATMTGADL LILLSDIDGL 

       190        200        210        220        230        240 
YTAPPHLDPD AKFLETIADI TPEIEAMAGG AASELSRGGM RTKIDAGKIA TGAGCAMIIA 

       250        260        270        280        290        300 
SGKTDRPLNA IANGARSSWF APSGTPVTAR KTWIAGQLQP AGELHVDEGA VTALGAGKSL 

       310        320        330        340        350        360 
LPAGVRKVTG HFGRGDTIAV IGPSGREIAR GLVGYDAEEA RQITGRKSAE IEAILGYAGR 

       370        380 
AAMVHRDDLV MTASVKTKAE KLKKDEAHA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000628 Genomic DNA. Translation: ACM28481.1.
RefSeqYP_002546415.1. NC_011985.1.

3D structure databases

ProteinModelPortalB9JER2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING311403.Arad_4877.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM28481; ACM28481; Arad_4877.
GeneID7370959.
KEGGara:Arad_4877.
PATRIC20808494. VBIAgrRad129173_6311.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0263.
HOGENOMHOG000246368.
KOK00931.
OMATVIHRDN.
OrthoDBEOG6PGK7G.
ProtClustDBPRK05429.

Enzyme and pathway databases

BioCycARAD311403:GHU8-3797-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_AGRRK
AccessionPrimary (citable) accession number: B9JER2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways