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B9JAG5 (B9JAG5_AGRRK) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022
Alternative name(s):
Glutamyl-tRNA synthetase HAMAP MF_00022
Gene names
Name:gltX1 EMBL ACM27780.1
Synonyms:gltX HAMAP MF_00022
Ordered Locus Names:Arad_3953
OrganismAgrobacterium radiobacter (strain K84 / ATCC BAA-868) [Complete proteome] [HAMAP]
Taxonomic identifier311403 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022

Subunit structure

Monomer By similarity. HAMAP MF_00022

Subcellular location

Cytoplasm By similarity HAMAP MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022 RuleBase RU003489

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Motif12 – 2211"HIGH" region By similarity HAMAP MF_00022
Motif253 – 2575"KMSKS" region By similarity HAMAP MF_00022

Sites

Binding site2561ATP By similarity HAMAP MF_00022

Sequences

Sequence LengthMass (Da)Tools
B9JAG5 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: A62109B8E54D5B7F

FASTA48554,466
        10         20         30         40         50         60 
MTTSGVRVRI APSPTGEPHV GTAYIALFNY LFAKKHGGEF ILRIEDTDAT RSTPEFETKV 

        70         80         90        100        110        120 
LDALKWCGLK WSEGPDIGGP YGPYRQSDRK DLYKPYVEKI VDAGHGFRCF CTPERLEQMR 

       130        140        150        160        170        180 
AAQRAAGLPP KYDGHCLNLS AEEVSSRVAA GEPHVVRMKI PTEGSCKFHD GVYGDVEIPW 

       190        200        210        220        230        240 
DAVDMQVLLK ADGMPTYHMA NVVDDHLMKI THVARGEEWL ASVPKHILIY RYLGLEPPVF 

       250        260        270        280        290        300 
MHLSLMRNAD KSKLSKRKNP TSISYYTALG YIPEALMNFL GLFFVQIAEG DELLTMDELA 

       310        320        330        340        350        360 
EKFDPENLSK AGAIFDIQKL DWLNGRWIRE KLSEEEFQQR VLTWAMENSR LQEGLKLSQS 

       370        380        390        400        410        420 
RISKLGELPD LTGFLFKSDL NLDPSAFARI KSTPEELLEI LNTVQPDLEK ILEWNVETIE 

       430        440        450        460        470        480 
AELRAIADRM GKKLKVIVAP LFVAVSGSSR SLPLFDSMAI LGRSVVRQRL KLAAQTVATL 


VGPKN

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000628 Genomic DNA. Translation: ACM27780.1.
RefSeqYP_002545711.1. NC_011985.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB9JAG5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7369996.
GenomeReviewsGene locus Arad_3953 in contig CP000628_GR.
KEGGara:Arad_3953.
PATRIC20807004. VBIAgrRad129173_5579.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAMAHIPLI.
ProtClustDBPRK01406.

Family and domain databases

HAMAPMF_00022_B. Glu_tRNA_synth_B.
[Tree]
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-synth_Ib_bac/mito.
IPR000924. Glu/Gln-tRNA-synth_Ib.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KOK01885.
PANTHERPTHR10119. Glu_tRNA-synt_1c. 1 hit.
PTHR10119:SF1. PTHR10119:SF1. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMsTIGR00464. GltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB9JAG5_AGRRK
AccessionPrimary (citable) accession number: B9JAG5
Entry history
Integrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info