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B9J9I5 (BIOB_AGRRK) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Arad_3690
OrganismAgrobacterium radiobacter (strain K84 / ATCC BAA-868) [Complete proteome] [HAMAP]
Taxonomic identifier311403 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence ACM27587.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 327327Biotin synthase HAMAP-Rule MF_01694
PRO_0000381186

Sites

Metal binding571Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1021Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1341Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1941Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2661Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B9J9I5 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 8D8C5A39B7C3EFC5

FASTA32735,133
        10         20         30         40         50         60 
MAADGTIRHD WAIDEIVALH NLPLLELVGR ANAVHRMHHD PNKVQKASLL SIKTGGCPEN 

        70         80         90        100        110        120 
CAYCPQSAHH REVDLTRDRL MDPESVVAMA ATARAAGAER FCMGAAWRQV RDGREFDAVI 

       130        140        150        160        170        180 
EMVKGVRALG MEACVTLGML KPHQAERLAA AGLTAYNHNL DTSPEFYGQI ITTRTYQDRL 

       190        200        210        220        230        240 
DTLATVRSFG IDLCCGGIIG MGETIRDRAS MLHILAAMEP HPESVPINAL VPVEGTPLAK 

       250        260        270        280        290        300 
RPVIAPLELV RMVATARLVM PASTVRLSAG RSNLNREAQI LCLVAGANSV FYGDTLLTTP 

       310        320 
NAGIGEDAEL FAAIGELDCD HVSASAA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000628 Genomic DNA. Translation: ACM27587.1. Different initiation.
RefSeqYP_002545517.1. NC_011985.1.

3D structure databases

ProteinModelPortalB9J9I5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING311403.Arad_3690.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM27587; ACM27587; Arad_3690.
GeneID7369228.
KEGGara:Arad_3690.
PATRIC20806607. VBIAgrRad129173_5382.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2463890.

Enzyme and pathway databases

BioCycARAD311403:GHU8-2868-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_AGRRK
AccessionPrimary (citable) accession number: B9J9I5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: February 19, 2014
This is version 42 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways