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B9J9I5

- BIOB_AGRRK

UniProt

B9J9I5 - BIOB_AGRRK

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Protein

Biotin synthase

Gene
bioB, Arad_3690
Organism
Agrobacterium radiobacter (strain K84 / ATCC BAA-868)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi102 – 1021Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi134 – 1341Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi194 – 1941Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi266 – 2661Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciARAD311403:GHU8-2868-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Arad_3690
OrganismiAgrobacterium radiobacter (strain K84 / ATCC BAA-868)
Taxonomic identifieri311403 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000001600: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Biotin synthaseUniRule annotationPRO_0000381186Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi311403.Arad_3690.

Structurei

3D structure databases

ProteinModelPortaliB9J9I5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B9J9I5-1 [UniParc]FASTAAdd to Basket

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MAADGTIRHD WAIDEIVALH NLPLLELVGR ANAVHRMHHD PNKVQKASLL    50
SIKTGGCPEN CAYCPQSAHH REVDLTRDRL MDPESVVAMA ATARAAGAER 100
FCMGAAWRQV RDGREFDAVI EMVKGVRALG MEACVTLGML KPHQAERLAA 150
AGLTAYNHNL DTSPEFYGQI ITTRTYQDRL DTLATVRSFG IDLCCGGIIG 200
MGETIRDRAS MLHILAAMEP HPESVPINAL VPVEGTPLAK RPVIAPLELV 250
RMVATARLVM PASTVRLSAG RSNLNREAQI LCLVAGANSV FYGDTLLTTP 300
NAGIGEDAEL FAAIGELDCD HVSASAA 327
Length:327
Mass (Da):35,133
Last modified:July 28, 2009 - v2
Checksum:i8D8C5A39B7C3EFC5
GO

Sequence cautioni

The sequence ACM27587.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000628 Genomic DNA. Translation: ACM27587.1. Different initiation.
RefSeqiWP_012652258.1. NC_011985.1.
YP_002545517.1. NC_011985.1.

Genome annotation databases

EnsemblBacteriaiACM27587; ACM27587; Arad_3690.
GeneIDi7369228.
KEGGiara:Arad_3690.
PATRICi20806607. VBIAgrRad129173_5382.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000628 Genomic DNA. Translation: ACM27587.1 . Different initiation.
RefSeqi WP_012652258.1. NC_011985.1.
YP_002545517.1. NC_011985.1.

3D structure databases

ProteinModelPortali B9J9I5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 311403.Arad_3690.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACM27587 ; ACM27587 ; Arad_3690 .
GeneIDi 7369228.
KEGGi ara:Arad_3690.
PATRICi 20806607. VBIAgrRad129173_5382.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci ARAD311403:GHU8-2868-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K84 / ATCC BAA-868.

Entry informationi

Entry nameiBIOB_AGRRK
AccessioniPrimary (citable) accession number: B9J9I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: September 3, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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