B9J7X6 (GLPK_AGRRK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Arad_3310

Organism

Agrobacterium radiobacter (strain K84 / ATCC BAA-868) [Complete proteome] [HAMAP]

Taxonomic identifier

311403 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	498 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 498	498	Glycerol kinase HAMAP MF_00186		PRO_1000124176
Regions
	Nucleotide binding	412 – 416	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	265	1	ATP By similarity
	Binding site	308	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9J7X6 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 3D0B917E5C654431	FASTA	498	54,218
	10 20 30 40 50 60 MGGYVLAIDQ GTTSTRAIVF DGNMKVAGVG QKEFTQHYPK PGWVEHDPEE IWASVLFTVG 70 80 90 100 110 120 KAITAAGITA KDIAAVGITN QRETVVVWDR ETGKPIHNAI VWQDRRTASY CDKLKRQDLE 130 140 150 160 170 180 KLFTRRTGLL LDPYFSGTKL SWMLANVKGA RARAAKGELC FGTIDTFLIW RLTGGKSFVT 190 200 210 220 230 240 DATNASRTLM YNIASNAWDE DLLEILRVPA AMLPEVKDCA ADFGITDASL FGATIPILGV 250 260 270 280 290 300 AGDQQAATIG QACFERGMMK STYGTGCFAL LNTGTDIVRS KNRLLTTIAY RLDGETTYAL 310 320 330 340 350 360 EGSIFIAGAA VQWLRDGLKA IKSAADSGAL AEKADPLQEV YLVPAFTGLG APHWDPDARG 370 380 390 400 410 420 AIFGLTRNTG PEEIVRAALE AVCYQSRDLL DAMHKDWRNG NGKETVLRVD GGMVASDWTM 430 440 450 460 470 480 QRLADLLDAP VDRPTILETT ALGAAWLAGS RAGVWPDREG FAKAWARDRR FEPAMDEKTR 490 SGKLKGWKDA VRRTLSAG

References

[1]

"Genome sequences of three Agrobacterium biovars help elucidate the evolution of multichromosome genomes in bacteria." Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K., Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., Otten L., Suen G., Welch R., Almeida N.F., Arnold F., Burton O.T., Du Z., Ewing A. , Godsy E., Heisel S., Houmiel K.L., Jhaveri J., Lu J., Miller N.M., Norton S., Chen Q., Phoolcharoen W., Ohlin V., Ondrusek D., Pride N., Stricklin S.L., Sun J., Wheeler C., Wilson L., Zhu H., Wood D.W. J. Bacteriol. 191:2501-2511(2009) [PubMed: 19251847] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K84 / ATCC BAA-868.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000628 Genomic DNA. Translation: ACM27297.1.
RefSeq	YP_002545227.1. NC_011985.1.
3D structure databases
ProteinModelPortal	B9J7X6.
ModBase	Search...
Protein-protein interaction databases
STRING	B9J7X6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7370165.
GenomeReviews	Gene locus Arad_3310 in contig CP000628_GR.
KEGG	ara:Arad_3310.
PATRIC	20806001. VBIAgrRad129173_5081.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CYQTRDL.
ProtClustDB	CLSK863056.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_AGRRK

Accession

Primary (citable) accession number: B9J7X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program