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B9J5G8 (B9J5G8_BACCQ) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UvrABC system protein B HAMAP-Rule MF_00204
Short name=Protein UvrB HAMAP-Rule MF_00204
Alternative name(s):
Excinuclease ABC subunit B HAMAP-Rule MF_00204
Gene names
Name:uvrB HAMAP-Rule MF_00204 EMBL ACM15389.1
Ordered Locus Names:BCQ_4989 EMBL ACM15389.1
OrganismBacillus cereus (strain Q1) [Complete proteome] [HAMAP] EMBL ACM15389.1
Taxonomic identifier361100 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group ›

Protein attributes

Sequence length658 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204 SAAS SAAS006935

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity. HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS006935

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS006935.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family. HAMAP-Rule MF_00204 RuleBase RU003587

Contains 1 UVR domain. HAMAP-Rule MF_00204 RuleBase RU003587 SAAS SAAS006935

Contains 1 helicase ATP-binding domain. HAMAP-Rule MF_00204 SAAS SAAS004807

Contains 1 helicase C-terminal domain. HAMAP-Rule MF_00204 SAAS SAAS004807

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain26 – 181156Helicase ATP-binding By similarity HAMAP-Rule MF_00204
Domain430 – 596167Helicase C-terminal By similarity HAMAP-Rule MF_00204
Domain622 – 65736UVR By similarity HAMAP-Rule MF_00204
Nucleotide binding39 – 468ATP By similarity HAMAP-Rule MF_00204
Motif92 – 11524Beta-hairpin By similarity HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
B9J5G8 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 770EE6A9D3521B4C

FASTA65875,168
        10         20         30         40         50         60 
MERQFEIVSA YSPQGDQPVA IEKLVEGINS GKKKQVLLGA TGTGKTFTIS NVIKEVQKPT 

        70         80         90        100        110        120 
LVMAHNKTLA GQLYSELKDF FPNNAVEYFV SYYDYYQPEA YVPQTDTFIE KDAQINDEID 

       130        140        150        160        170        180 
KLRHSATSAL FERDDVIIVA SVSCIYGLGS PEEYRELVVS LRVGMEKDRN QLLRELVDVQ 

       190        200        210        220        230        240 
YGRNDIDFKR GTFRVRGDVV EIFPASLDEH CIRIEFFGDE IDRIREVNAL TGEVLAERDH 

       250        260        270        280        290        300 
VAIFPASHFV TREEKMKVAI ENIEKELEER LKELNDNGKL LEAQRIEQRT RYDLEMMREM 

       310        320        330        340        350        360 
GFCSGIENYS RHLTLRPAGA TPYTLLDYFP EDFLIVMDES HVSVPQVRAM YNGDQARKQV 

       370        380        390        400        410        420 
LVDHGFRLPS ALDNRPLTFD EFEEKTNQVI YVSATPGPYE LEQSPEVIEQ IIRPTGLLDP 

       430        440        450        460        470        480 
PIDIRPIEGQ IDDLLGEIQD RIAKNERVLI TTLTKKMSED LTDYLKDVGI KVNYLHSEVK 

       490        500        510        520        530        540 
TLERIEIIRD LRLGKFDVLV GINLLREGLD IPEVSLVAIL DADKEGFLRS ERSLIQTIGR 

       550        560        570        580        590        600 
AARNENGRVI MYADRITRSM GIAIEETKRR RSIQEAYNEE HGITPKTIQK GVRDVIRATT 

       610        620        630        640        650 
AAEETETYEA TPAKKMTKKE REKTIAKMEA EMKEAAKALD FERAAELRDL LLELKAEG

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References

[1]"Complete genome sequence of the extremophilic Bacillus cereus strain Q1 with industrial applications."
Xiong Z., Jiang Y., Qi D., Lu H., Yang F., Yang J., Chen L., Sun L., Xu X., Xue Y., Zhu Y., Jin Q.
J. Bacteriol. 191:1120-1121(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Q1 EMBL ACM15389.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000227 Genomic DNA. Translation: ACM15389.1.
RefSeqYP_002532678.1. NC_011969.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING361100.BCQ_4989.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACM15389; ACM15389; BCQ_4989.
GeneID7376276.
KEGGbcq:BCQ_4989.
PATRIC18917791. VBIBacCer120424_5034.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0556.
HOGENOMHOG000073580.
KOK03702.
OMACIYGLGI.
ProtClustDBPRK05298.

Enzyme and pathway databases

BioCycBCER361100:GJ7M-4976-MONOMER.

Family and domain databases

Gene3D4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF46600. UvrB_C. 1 hit.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB9J5G8_BACCQ
AccessionPrimary (citable) accession number: B9J5G8
Entry history
Integrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: May 29, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info