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Protein

Processive diacylglycerol beta-glucosyltransferase

Gene

ugtP

Organism
Bacillus cereus (strain Q1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Processive glucosyltransferase involved in the biosynthesis of both the bilayer- and non-bilayer-forming membrane glucolipids. Is able to successively transfer up to three glucosyl residues to diacylglycerol (DAG), thereby catalyzing the formation of beta-monoglucosyl-DAG (3-O-(beta-D-glucopyranosyl)-1,2-diacyl-sn-glycerol), beta-diglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol) and beta-triglucosyl-DAG (3-O-(beta-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl-beta-(1->6)-D-glucopyranosyl)-1,2-diacyl-sn-glycerol). Beta-diglucosyl-DAG is the predominant glycolipid found in Bacillales and is also used as a membrane anchor for lipoteichoic acid (LTA).UniRule annotation

Catalytic activityi

UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP.
UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol.UniRule annotation

Pathwayi: diglucosyl-diacylglycerol biosynthesis

This protein is involved in the pathway diglucosyl-diacylglycerol biosynthesis, which is part of Glycolipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway diglucosyl-diacylglycerol biosynthesis and in Glycolipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciBCER361100:GJ7M-529-MONOMER.
UniPathwayiUPA00894.

Names & Taxonomyi

Protein namesi
Recommended name:
Processive diacylglycerol beta-glucosyltransferaseUniRule annotation (EC:2.4.1.315)
Alternative name(s):
Beta-diglucosyldiacylglycerol synthaseUniRule annotation
Short name:
Beta-DGSUniRule annotation
Short name:
DGlcDAG synthaseUniRule annotation
Short name:
Glc2-DAG synthaseUniRule annotation
Beta-gentiobiosyldiacylglycerol synthaseUniRule annotation
Beta-monoglucosyldiacylglycerol synthaseUniRule annotation
Short name:
Beta-MGSUniRule annotation
Short name:
MGlcDAG synthaseUniRule annotation
Beta-triglucosyldiacylglycerol synthaseUniRule annotation
Short name:
TGlcDAG synthaseUniRule annotation
Diglucosyl diacylglycerol synthase (1,6-linking)
Glucosyl-beta-1,6-glucosyldiacylglycerol synthaseUniRule annotation
UDP glucosyltransferaseUniRule annotation
UDP-glucose:1,2-diacylglycerol-3-beta-D-glucosyltransferaseUniRule annotation
Gene namesi
Name:ugtPUniRule annotation
Ordered Locus Names:BCQ_0539
OrganismiBacillus cereus (strain Q1)
Taxonomic identifieri361100 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000441 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Processive diacylglycerol beta-glucosyltransferasePRO_1000165234Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family. UgtP subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000221565.
KOiK03429.
OMAiEPAGHIV.
OrthoDBiEOG61VZFD.

Family and domain databases

HAMAPiMF_01280. Diacylglyc_glucosyltr.
InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
IPR023589. Pro_diacylglycrl_glcsylTrfase.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9J2U2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKNPKVLIL TAHYGNGHVQ VAKTLEQTFR QKGIKDVIVC DLFGESHPVI
60 70 80 90 100
TDITKYLYLK SYTIGKELYR LFYYGVEKIY DKKIASWYAN FGRKRLKLLL
110 120 130 140 150
QAEKPDIVIN TFPIIAVPEL KKQTGISIPV YNVLTDFCVH KIWIHREVDR
160 170 180 190 200
YFVATDHVKK VMVDIGVPAE QIVETGIPIR SSFELKINSD IIYNKYQLCK
210 220 230 240 250
NKKILLIVAG AHGVLGSVKE LCQSFMSVPD LQVVVVCGKN EALKQDLLGL
260 270 280 290 300
QEKNPDALKV FGYVENIDEL FRVTSCMITK PGGITLSEAA ALQVPVILYK
310 320 330 340 350
PVPGQENENA MYFERKGAAV VIRDDSEVFA KTEALLQDDM RLLQMKEAMK
360 370 380
SIYRPEPADH IVDTILAENH VEPNHIPIKS PALAQSFT
Length:388
Mass (Da):43,834
Last modified:March 24, 2009 - v1
Checksum:iB67FB2017B18DA4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000227 Genomic DNA. Translation: ACM11011.1.
RefSeqiWP_000594713.1. NC_011969.1.

Genome annotation databases

EnsemblBacteriaiACM11011; ACM11011; BCQ_0539.
KEGGibcq:BCQ_0539.
PATRICi18908543. VBIBacCer120424_0486.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000227 Genomic DNA. Translation: ACM11011.1.
RefSeqiWP_000594713.1. NC_011969.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACM11011; ACM11011; BCQ_0539.
KEGGibcq:BCQ_0539.
PATRICi18908543. VBIBacCer120424_0486.

Phylogenomic databases

HOGENOMiHOG000221565.
KOiK03429.
OMAiEPAGHIV.
OrthoDBiEOG61VZFD.

Enzyme and pathway databases

UniPathwayiUPA00894.
BioCyciBCER361100:GJ7M-529-MONOMER.

Family and domain databases

HAMAPiMF_01280. Diacylglyc_glucosyltr.
InterProiIPR009695. Diacylglyc_glucosyltr_N.
IPR007235. Glyco_trans_28_C.
IPR023589. Pro_diacylglycrl_glcsylTrfase.
[Graphical view]
PfamiPF04101. Glyco_tran_28_C. 1 hit.
PF06925. MGDG_synth. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the extremophilic Bacillus cereus strain Q1 with industrial applications."
    Xiong Z., Jiang Y., Qi D., Lu H., Yang F., Yang J., Chen L., Sun L., Xu X., Xue Y., Zhu Y., Jin Q.
    J. Bacteriol. 191:1120-1121(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Q1.

Entry informationi

Entry nameiUGTP_BACCQ
AccessioniPrimary (citable) accession number: B9J2U2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: November 11, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.