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B9I666

- LISC1_POPTR

UniProt

B9I666 - LISC1_POPTR

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Protein
Lipoyl synthase 1, chloroplastic
Gene
LIP1P-1, POPTRDRAFT_774018
Organism
Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi107 – 1071Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi112 – 1121Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi118 – 1181Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi142 – 1421Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi145 – 1451Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 1, chloroplastic (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase 1
Short name:
LS 1
Short name:
Lip-syn 1
Lipoate synthase, plastidial 1
Short name:
LIP1p 1
Lipoic acid synthase 1
Gene namesi
Name:LIP1P-1
ORF Names:POPTRDRAFT_774018
OrganismiPopulus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic identifieri3694 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus
ProteomesiUP000006729: Linkage group LGXIII, UP000006729: Unassembled WGS sequence

Subcellular locationi

Plastidchloroplast Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535Chloroplast Reviewed prediction
Add
BLAST
Chaini36 – 376341Lipoyl synthase 1, chloroplasticUniRule annotation
PRO_0000398867Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi3694.fgenesh4_pg.C_LG_XIII000472.

Structurei

3D structure databases

ProteinModelPortaliB9I666.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiNVKKPEW.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B9I666-1 [UniParc]FASTAAdd to Basket

« Hide

MIEQSLSKPS FSLSIPIPQP PKSKSSFLCS YSKIRCESVD YPSSSKIDAK    50
HPQISSINSN GGGKMGSYTG RDPNVKKPEW LRQKAPQGER YDEVKESLSR 100
LKLNTVCQEA QCPNIGECWN GGGDGIATAT IMVLGDTCTR GCRFCAVKTS 150
RNPPPPDPME PLNTALAIAS WGVDYIVITS VDRDDLPDGG SGHFAQTVRA 200
MKELKPEIMV ECLTSDFRGD LKAVDTLVHS GLDVFAHNVE TVKRLQRIVR 250
DPRAGYEQSL SVLKHAKISK KGMITKTSIM LGLGESDNEV KEAMADLRAI 300
GVDILTFGQY LQPTPLHLTV KEYVTPEKFA FWKEYGESIG FRYVASGPLV 350
RSSYRAGELF VKTMVKESVK EAAAIS 376
Length:376
Mass (Da):41,273
Last modified:March 24, 2009 - v1
Checksum:i2DBD5AE7DBFA7212
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CM000349 Genomic DNA. Translation: EEE95835.1.
RefSeqiXP_002319912.1. XM_002319876.2.

Genome annotation databases

EnsemblPlantsiPOPTR_0013s13960.1; POPTR_0013s13960.1; POPTR_0013s13960.
GeneIDi7494421.
KEGGipop:POPTR_0013s13960g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CM000349 Genomic DNA. Translation: EEE95835.1 .
RefSeqi XP_002319912.1. XM_002319876.2.

3D structure databases

ProteinModelPortali B9I666.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3694.fgenesh4_pg.C_LG_XIII000472.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi POPTR_0013s13960.1 ; POPTR_0013s13960.1 ; POPTR_0013s13960 .
GeneIDi 7494421.
KEGGi pop:POPTR_0013s13960g.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi NVKKPEW.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)."
    Tuskan G.A., Difazio S., Jansson S., Bohlmann J., Grigoriev I., Hellsten U., Putnam N., Ralph S., Rombauts S., Salamov A., Schein J., Sterck L., Aerts A., Bhalerao R.R., Bhalerao R.P., Blaudez D., Boerjan W., Brun A.
    , Brunner A., Busov V., Campbell M., Carlson J., Chalot M., Chapman J., Chen G.-L., Cooper D., Coutinho P.M., Couturier J., Covert S., Cronk Q., Cunningham R., Davis J., Degroeve S., Dejardin A., dePamphilis C.W., Detter J., Dirks B., Dubchak I., Duplessis S., Ehlting J., Ellis B., Gendler K., Goodstein D., Gribskov M., Grimwood J., Groover A., Gunter L., Hamberger B., Heinze B., Helariutta Y., Henrissat B., Holligan D., Holt R., Huang W., Islam-Faridi N., Jones S., Jones-Rhoades M., Jorgensen R., Joshi C., Kangasjaervi J., Karlsson J., Kelleher C., Kirkpatrick R., Kirst M., Kohler A., Kalluri U., Larimer F., Leebens-Mack J., Leple J.-C., Locascio P., Lou Y., Lucas S., Martin F., Montanini B., Napoli C., Nelson D.R., Nelson C., Nieminen K., Nilsson O., Pereda V., Peter G., Philippe R., Pilate G., Poliakov A., Razumovskaya J., Richardson P., Rinaldi C., Ritland K., Rouze P., Ryaboy D., Schmutz J., Schrader J., Segerman B., Shin H., Siddiqui A., Sterky F., Terry A., Tsai C.-J., Uberbacher E., Unneberg P., Vahala J., Wall K., Wessler S., Yang G., Yin T., Douglas C., Marra M., Sandberg G., Van de Peer Y., Rokhsar D.S.
    Science 313:1596-1604(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nisqually.
  2. Cited for: GENOME REANNOTATION.
    Strain: cv. Nisqually.

Entry informationi

Entry nameiLISC1_POPTR
AccessioniPrimary (citable) accession number: B9I666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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