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Protein

Lipoyl synthase 1, chloroplastic

Gene

LIP1P-1

Organism
Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase 1, chloroplastic (LIP1P-1), Lipoyl synthase, chloroplastic (LIP1P), Lipoyl synthase, chloroplastic (LIP1P), Lipoyl synthase, chloroplastic (LIP1P), Lipoyl synthase, mitochondrial (LIP1), Lipoyl synthase, mitochondrial (LIP1), Lipoyl synthase, mitochondrial (POPTR_0005s08840g), Lipoyl synthase, mitochondrial (POPTR_005G086200v3), Lipoyl synthase, mitochondrial (POPTR_0005s08840g), Lipoyl synthase, mitochondrial (LIP1), Lipoyl synthase 2, chloroplastic (LIP1P-2), Lipoyl synthase, mitochondrial (POPTR_0007s06900g)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi112Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi118Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi138Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi142Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi145Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 1, chloroplastic (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthase 1UniRule annotation
Short name:
LS 1UniRule annotation
Short name:
Lip-syn 1UniRule annotation
Lipoate synthase, plastidial 1UniRule annotation
Short name:
LIP1p 1UniRule annotation
Lipoic acid synthase 1UniRule annotation
Gene namesi
Name:LIP1P-1UniRule annotation
ORF Names:POPTRDRAFT_774018
OrganismiPopulus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa)
Taxonomic identifieri3694 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesSalicaceaeSaliceaePopulus
Proteomesi
  • UP000006729 Componentsi: Chromosome 13, Linkage group LGXIII, Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35ChloroplastUniRule annotationAdd BLAST35
ChainiPRO_000039886736 – 376Lipoyl synthase 1, chloroplasticAdd BLAST341

Proteomic databases

PRIDEiB9I666

Interactioni

Protein-protein interaction databases

STRINGi3694.POPTR_0013s13960.1

Structurei

3D structure databases

ProteinModelPortaliB9I666
SMRiB9I666
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
HOGENOMiHOG000235998
InParanoidiB9I666
KOiK03644
OMAiPYCDIDF
OrthoDBiEOG093604CP

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
MF_03129 Lipoyl_synth_plantC, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR027526 Lipoyl_synth_chlpt
IPR007197 rSAM
PANTHERiPTHR10949:SF14 PTHR10949:SF14, 1 hit
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B9I666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEQSLSKPS FSLSIPIPQP PKSKSSFLCS YSKIRCESVD YPSSSKIDAK
60 70 80 90 100
HPQISSINSN GGGKMGSYTG RDPNVKKPEW LRQKAPQGER YDEVKESLSR
110 120 130 140 150
LKLNTVCQEA QCPNIGECWN GGGDGIATAT IMVLGDTCTR GCRFCAVKTS
160 170 180 190 200
RNPPPPDPME PLNTALAIAS WGVDYIVITS VDRDDLPDGG SGHFAQTVRA
210 220 230 240 250
MKELKPEIMV ECLTSDFRGD LKAVDTLVHS GLDVFAHNVE TVKRLQRIVR
260 270 280 290 300
DPRAGYEQSL SVLKHAKISK KGMITKTSIM LGLGESDNEV KEAMADLRAI
310 320 330 340 350
GVDILTFGQY LQPTPLHLTV KEYVTPEKFA FWKEYGESIG FRYVASGPLV
360 370
RSSYRAGELF VKTMVKESVK EAAAIS
Length:376
Mass (Da):41,273
Last modified:March 24, 2009 - v1
Checksum:i2DBD5AE7DBFA7212
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000349 Genomic DNA Translation: EEE95835.1
RefSeqiXP_002319912.1, XM_002319876.2

Genome annotation databases

EnsemblPlantsiPOPTR_0013s13960.1; POPTR_0013s13960.1; POPTR_0013s13960
GeneIDi7494421
GrameneiPOPTR_0013s13960.1; POPTR_0013s13960.1; POPTR_0013s13960
KEGGipop:POPTR_0013s13960g

Similar proteinsi

Entry informationi

Entry nameiLISC1_POPTR
AccessioniPrimary (citable) accession number: B9I666
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 24, 2009
Last modified: May 23, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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