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B9FHH2 (BGL20_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 20

Short name=Os5bglu20
EC=3.2.1.21
Gene names
Name:BGLU20
Ordered Locus Names:Os05g0365700, LOC_Os05g30280
ORF Names:OsJ_18258, OSJNBa0090H02.5, OSJNBb0111K12.13
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length517 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence caution

The sequence AAS79741.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: B9FHH2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: B9FHH2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.
     368-381: ATKNDTPTPEFLPG → GSKFVQICFYCVFF
     382-517: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 517489Beta-glucosidase 20
PRO_0000390337

Regions

Region458 – 4592Substrate binding By similarity

Sites

Active site1961Proton donor By similarity
Active site4091Nucleophile By similarity
Binding site501Substrate By similarity
Binding site1501Substrate By similarity
Binding site3391Substrate By similarity
Binding site4511Substrate By similarity

Amino acid modifications

Glycosylation2221N-linked (GlcNAc...) Potential
Glycosylation2271N-linked (GlcNAc...) Potential
Glycosylation4021N-linked (GlcNAc...) Potential
Glycosylation4101N-linked (GlcNAc...) Potential
Disulfide bond215 ↔ 223 By similarity

Natural variations

Alternative sequence1 – 199199Missing in isoform 2.
VSP_038505
Alternative sequence368 – 38114ATKND…EFLPG → GSKFVQICFYCVFF in isoform 2.
VSP_038506
Alternative sequence382 – 517136Missing in isoform 2.
VSP_038507

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 5EA38C45C4486EEA

FASTA51758,195
        10         20         30         40         50         60 
MERRPLHLHL LLFFSAWLLL LLLQGVSSLQ FRREDFPDGF AFGAGTAAYQ YEGAAAEDGR 

        70         80         90        100        110        120 
TPSIWDTYTH SGRHPEDGTG DVASDGYHKY KEDVKLMTEI GLEAYRFTIS WSRLIPSGRG 

       130        140        150        160        170        180 
AVNPKGLQFY NNMINELVKA GIQIQVALYH SDLPQSLQDE YGGWINPKIV DDFTAYADVC 

       190        200        210        220        230        240 
FREFGDRVAH WTTVLEPNVM AQGCYDTGIL PPNHCSYPFG NNCTGGNSTV EPYLFIHHNL 

       250        260        270        280        290        300 
LAHASAVRLY REKYQVAQKG IIGINMYSLW FYPLTDSAED IGATERAKQF MYGWILHPLV 

       310        320        330        340        350        360 
FGDYPETIKK VVGSRLPFFS NHESELVTNA FDFIGLNHYS SVYTSNNNNV VKAPLQDLTA 

       370        380        390        400        410        420 
DIATLFRATK NDTPTPEFLP GNTVDPQGLE NALEYIRENY GNLTIYIQEN GSGAPDGTLD 

       430        440        450        460        470        480 
DVERINYLQK YIAATLKAIR NGANVKGYSM WSFIDIYEIF GGYNSWHYGL VAVDFGSTER 

       490        500        510 
RRQPRRSASW YSDFLKNNAP IRVEDGSFVS AASHAQL 

« Hide

Isoform 2 [UniParc].

Checksum: 29A8FF4F923AD247
Show »

FASTA18220,574

References

« Hide 'large scale' references
[1]"A fine physical map of the rice chromosome 5."
Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.
Mol. Genet. Genomics 274:337-345(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Nipponbare.
[4]"Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC121366 Genomic DNA. Translation: AAS79741.1. Sequence problems.
AC137618 Genomic DNA. Translation: AAV31354.1.
CM000142 Genomic DNA. Translation: EEE63445.1.
AK120998 mRNA. Translation: BAH00265.1.

3D structure databases

ProteinModelPortalB9FHH2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os05g30280.3.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneB9FHH2.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
OMANKFINCT.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL20_ORYSJ
AccessionPrimary (citable) accession number: B9FHH2
Secondary accession number(s): Q60DY2, Q75IS8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries