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B9FHH2

- BGL20_ORYSJ

UniProt

B9FHH2 - BGL20_ORYSJ

Protein

Beta-glucosidase 20

Gene

BGLU20

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501SubstrateBy similarity
    Binding sitei150 – 1501SubstrateBy similarity
    Active sitei196 – 1961Proton donorBy similarity
    Binding sitei339 – 3391SubstrateBy similarity
    Active sitei409 – 4091NucleophileBy similarity
    Binding sitei451 – 4511SubstrateBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 20 (EC:3.2.1.21)
    Short name:
    Os5bglu20
    Gene namesi
    Name:BGLU20
    Ordered Locus Names:Os05g0365700, LOC_Os05g30280
    ORF Names:OsJ_18258, OSJNBa0090H02.5, OSJNBb0111K12.13
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 5

    Organism-specific databases

    GrameneiB9FHH2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 517489Beta-glucosidase 20PRO_0000390337Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi215 ↔ 223By similarity
    Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi410 – 4101N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi39947.LOC_Os05g30280.3.

    Structurei

    3D structure databases

    ProteinModelPortaliB9FHH2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni458 – 4592Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    OMAiNKFINCT.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: B9FHH2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MERRPLHLHL LLFFSAWLLL LLLQGVSSLQ FRREDFPDGF AFGAGTAAYQ    50
    YEGAAAEDGR TPSIWDTYTH SGRHPEDGTG DVASDGYHKY KEDVKLMTEI 100
    GLEAYRFTIS WSRLIPSGRG AVNPKGLQFY NNMINELVKA GIQIQVALYH 150
    SDLPQSLQDE YGGWINPKIV DDFTAYADVC FREFGDRVAH WTTVLEPNVM 200
    AQGCYDTGIL PPNHCSYPFG NNCTGGNSTV EPYLFIHHNL LAHASAVRLY 250
    REKYQVAQKG IIGINMYSLW FYPLTDSAED IGATERAKQF MYGWILHPLV 300
    FGDYPETIKK VVGSRLPFFS NHESELVTNA FDFIGLNHYS SVYTSNNNNV 350
    VKAPLQDLTA DIATLFRATK NDTPTPEFLP GNTVDPQGLE NALEYIRENY 400
    GNLTIYIQEN GSGAPDGTLD DVERINYLQK YIAATLKAIR NGANVKGYSM 450
    WSFIDIYEIF GGYNSWHYGL VAVDFGSTER RRQPRRSASW YSDFLKNNAP 500
    IRVEDGSFVS AASHAQL 517
    Length:517
    Mass (Da):58,195
    Last modified:March 24, 2009 - v1
    Checksum:i5EA38C45C4486EEA
    GO
    Isoform 2 (identifier: B9FHH2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-199: Missing.
         368-381: ATKNDTPTPEFLPG → GSKFVQICFYCVFF
         382-517: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:182
    Mass (Da):20,574
    Checksum:i29A8FF4F923AD247
    GO

    Sequence cautioni

    The sequence AAS79741.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 199199Missing in isoform 2. 1 PublicationVSP_038505Add
    BLAST
    Alternative sequencei368 – 38114ATKND…EFLPG → GSKFVQICFYCVFF in isoform 2. 1 PublicationVSP_038506Add
    BLAST
    Alternative sequencei382 – 517136Missing in isoform 2. 1 PublicationVSP_038507Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC121366 Genomic DNA. Translation: AAS79741.1. Sequence problems.
    AC137618 Genomic DNA. Translation: AAV31354.1.
    CM000142 Genomic DNA. Translation: EEE63445.1.
    AK120998 mRNA. Translation: BAH00265.1.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC121366 Genomic DNA. Translation: AAS79741.1 . Sequence problems.
    AC137618 Genomic DNA. Translation: AAV31354.1 .
    CM000142 Genomic DNA. Translation: EEE63445.1 .
    AK120998 mRNA. Translation: BAH00265.1 .

    3D structure databases

    ProteinModelPortali B9FHH2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os05g30280.3.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei B9FHH2.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    OMAi NKFINCT.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Nipponbare.
    4. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
      Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
      BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiBGL20_ORYSJ
    AccessioniPrimary (citable) accession number: B9FHH2
    Secondary accession number(s): Q60DY2, Q75IS8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3