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Protein

Beta-glucosidase 20

Gene

BGLU20

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50SubstrateBy similarity1
Binding sitei150SubstrateBy similarity1
Active sitei196Proton donorBy similarity1
Binding sitei339SubstrateBy similarity1
Active sitei409NucleophileBy similarity1
Binding sitei451SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 20 (EC:3.2.1.21)
Short name:
Os5bglu20
Gene namesi
Name:BGLU20
Ordered Locus Names:Os05g0365700, LOC_Os05g30280
ORF Names:OsJ_18258, OSJNBa0090H02.5, OSJNBb0111K12.13
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000039033729 – 517Beta-glucosidase 20Add BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi215 ↔ 223By similarity
Glycosylationi222N-linked (GlcNAc...)Sequence analysis1
Glycosylationi227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi402N-linked (GlcNAc...)Sequence analysis1
Glycosylationi410N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiB9FHH2.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os05g30280.3.

Structurei

3D structure databases

ProteinModelPortaliB9FHH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni458 – 459Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiB9FHH2.
KOiK01188.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: B9FHH2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERRPLHLHL LLFFSAWLLL LLLQGVSSLQ FRREDFPDGF AFGAGTAAYQ
60 70 80 90 100
YEGAAAEDGR TPSIWDTYTH SGRHPEDGTG DVASDGYHKY KEDVKLMTEI
110 120 130 140 150
GLEAYRFTIS WSRLIPSGRG AVNPKGLQFY NNMINELVKA GIQIQVALYH
160 170 180 190 200
SDLPQSLQDE YGGWINPKIV DDFTAYADVC FREFGDRVAH WTTVLEPNVM
210 220 230 240 250
AQGCYDTGIL PPNHCSYPFG NNCTGGNSTV EPYLFIHHNL LAHASAVRLY
260 270 280 290 300
REKYQVAQKG IIGINMYSLW FYPLTDSAED IGATERAKQF MYGWILHPLV
310 320 330 340 350
FGDYPETIKK VVGSRLPFFS NHESELVTNA FDFIGLNHYS SVYTSNNNNV
360 370 380 390 400
VKAPLQDLTA DIATLFRATK NDTPTPEFLP GNTVDPQGLE NALEYIRENY
410 420 430 440 450
GNLTIYIQEN GSGAPDGTLD DVERINYLQK YIAATLKAIR NGANVKGYSM
460 470 480 490 500
WSFIDIYEIF GGYNSWHYGL VAVDFGSTER RRQPRRSASW YSDFLKNNAP
510
IRVEDGSFVS AASHAQL
Length:517
Mass (Da):58,195
Last modified:March 24, 2009 - v1
Checksum:i5EA38C45C4486EEA
GO
Isoform 2 (identifier: B9FHH2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.
     368-381: ATKNDTPTPEFLPG → GSKFVQICFYCVFF
     382-517: Missing.

Note: No experimental confirmation available.
Show »
Length:182
Mass (Da):20,574
Checksum:i29A8FF4F923AD247
GO

Sequence cautioni

The sequence AAS79741 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0385051 – 199Missing in isoform 2. 1 PublicationAdd BLAST199
Alternative sequenceiVSP_038506368 – 381ATKND…EFLPG → GSKFVQICFYCVFF in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_038507382 – 517Missing in isoform 2. 1 PublicationAdd BLAST136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC121366 Genomic DNA. Translation: AAS79741.1. Sequence problems.
AC137618 Genomic DNA. Translation: AAV31354.1.
AP014961 Genomic DNA. No translation available.
CM000142 Genomic DNA. Translation: EEE63445.1.
AK120998 mRNA. Translation: BAH00265.1.
RefSeqiXP_015639295.1. XM_015783809.1. [B9FHH2-1]
UniGeneiOs.74437.

Genome annotation databases

GeneIDi9270758.
KEGGiosa:9270758.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC121366 Genomic DNA. Translation: AAS79741.1. Sequence problems.
AC137618 Genomic DNA. Translation: AAV31354.1.
AP014961 Genomic DNA. No translation available.
CM000142 Genomic DNA. Translation: EEE63445.1.
AK120998 mRNA. Translation: BAH00265.1.
RefSeqiXP_015639295.1. XM_015783809.1. [B9FHH2-1]
UniGeneiOs.74437.

3D structure databases

ProteinModelPortaliB9FHH2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os05g30280.3.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiB9FHH2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9270758.
KEGGiosa:9270758.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
InParanoidiB9FHH2.
KOiK01188.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGL20_ORYSJ
AccessioniPrimary (citable) accession number: B9FHH2
Secondary accession number(s): Q60DY2, Q75IS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 24, 2009
Last modified: November 30, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.