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B9FHH2

- BGL20_ORYSJ

UniProt

B9FHH2 - BGL20_ORYSJ

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Protein

Beta-glucosidase 20

Gene

BGLU20

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501SubstrateBy similarity
Binding sitei150 – 1501SubstrateBy similarity
Active sitei196 – 1961Proton donorBy similarity
Binding sitei339 – 3391SubstrateBy similarity
Active sitei409 – 4091NucleophileBy similarity
Binding sitei451 – 4511SubstrateBy similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 20 (EC:3.2.1.21)
Short name:
Os5bglu20
Gene namesi
Name:BGLU20
Ordered Locus Names:Os05g0365700, LOC_Os05g30280
ORF Names:OsJ_18258, OSJNBa0090H02.5, OSJNBb0111K12.13
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 5

Organism-specific databases

GrameneiB9FHH2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 517489Beta-glucosidase 20PRO_0000390337Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi215 ↔ 223By similarity
Glycosylationi222 – 2221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi410 – 4101N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os05g30280.3.

Structurei

3D structure databases

ProteinModelPortaliB9FHH2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni458 – 4592Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiB9FHH2.
OMAiNKFINCT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: B9FHH2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERRPLHLHL LLFFSAWLLL LLLQGVSSLQ FRREDFPDGF AFGAGTAAYQ
60 70 80 90 100
YEGAAAEDGR TPSIWDTYTH SGRHPEDGTG DVASDGYHKY KEDVKLMTEI
110 120 130 140 150
GLEAYRFTIS WSRLIPSGRG AVNPKGLQFY NNMINELVKA GIQIQVALYH
160 170 180 190 200
SDLPQSLQDE YGGWINPKIV DDFTAYADVC FREFGDRVAH WTTVLEPNVM
210 220 230 240 250
AQGCYDTGIL PPNHCSYPFG NNCTGGNSTV EPYLFIHHNL LAHASAVRLY
260 270 280 290 300
REKYQVAQKG IIGINMYSLW FYPLTDSAED IGATERAKQF MYGWILHPLV
310 320 330 340 350
FGDYPETIKK VVGSRLPFFS NHESELVTNA FDFIGLNHYS SVYTSNNNNV
360 370 380 390 400
VKAPLQDLTA DIATLFRATK NDTPTPEFLP GNTVDPQGLE NALEYIRENY
410 420 430 440 450
GNLTIYIQEN GSGAPDGTLD DVERINYLQK YIAATLKAIR NGANVKGYSM
460 470 480 490 500
WSFIDIYEIF GGYNSWHYGL VAVDFGSTER RRQPRRSASW YSDFLKNNAP
510
IRVEDGSFVS AASHAQL
Length:517
Mass (Da):58,195
Last modified:March 24, 2009 - v1
Checksum:i5EA38C45C4486EEA
GO
Isoform 2 (identifier: B9FHH2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-199: Missing.
     368-381: ATKNDTPTPEFLPG → GSKFVQICFYCVFF
     382-517: Missing.

Note: No experimental confirmation available.

Show »
Length:182
Mass (Da):20,574
Checksum:i29A8FF4F923AD247
GO

Sequence cautioni

The sequence AAS79741.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 199199Missing in isoform 2. 1 PublicationVSP_038505Add
BLAST
Alternative sequencei368 – 38114ATKND…EFLPG → GSKFVQICFYCVFF in isoform 2. 1 PublicationVSP_038506Add
BLAST
Alternative sequencei382 – 517136Missing in isoform 2. 1 PublicationVSP_038507Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC121366 Genomic DNA. Translation: AAS79741.1. Sequence problems.
AC137618 Genomic DNA. Translation: AAV31354.1.
CM000142 Genomic DNA. Translation: EEE63445.1.
AK120998 mRNA. Translation: BAH00265.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC121366 Genomic DNA. Translation: AAS79741.1 . Sequence problems.
AC137618 Genomic DNA. Translation: AAV31354.1 .
CM000142 Genomic DNA. Translation: EEE63445.1 .
AK120998 mRNA. Translation: BAH00265.1 .

3D structure databases

ProteinModelPortali B9FHH2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39947.LOC_Os05g30280.3.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei B9FHH2.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi B9FHH2.
OMAi NKFINCT.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Nipponbare.
  4. "Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 beta-glucosidase."
    Opassiri R., Pomthong B., Onkoksoong T., Akiyama T., Esen A., Ketudat Cairns J.R.
    BMC Plant Biol. 6:33-33(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL20_ORYSJ
AccessioniPrimary (citable) accession number: B9FHH2
Secondary accession number(s): Q60DY2, Q75IS8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: March 24, 2009
Last modified: October 29, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3