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Protein

TATA element modulatory factor

Gene

Tmf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potential coactivator of the androgen receptor. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER (By similarity). Mediates STAT3 degradation.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • acrosome assembly Source: MGI
  • cellular response to organic cyclic compound Source: MGI
  • defense response to bacterium Source: MGI
  • flagellated sperm motility Source: MGI
  • Leydig cell differentiation Source: MGI
  • luteinizing hormone secretion Source: MGI
  • male gonad development Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of gene expression Source: MGI
  • positive regulation of cytokine production Source: MGI
  • positive regulation of testosterone secretion Source: MGI
  • regulation of proteasomal protein catabolic process Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • spermatid development Source: MGI
  • spermatid nucleus differentiation Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA element modulatory factor
Short name:
TMF
Alternative name(s):
Androgen receptor coactivator 160 kDa protein
Androgen receptor-associated protein of 160 kDa
Gene namesi
Name:Tmf1
Synonyms:Ara160, Gm153
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2684999. Tmf1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003767971 – 1091TATA element modulatory factorAdd BLAST1091

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73PhosphoserineBy similarity1
Modified residuei78PhosphoserineBy similarity1
Modified residuei112PhosphoserineBy similarity1
Modified residuei136PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei324PhosphoserineCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei329PhosphoserineCombined sources1
Modified residuei334PhosphoserineBy similarity1
Modified residuei340PhosphoserineCombined sources1
Modified residuei357PhosphoserineCombined sources1
Modified residuei411PhosphoserineCombined sources1
Modified residuei540PhosphoserineBy similarity1
Modified residuei923PhosphoserineBy similarity1
Modified residuei926PhosphoserineCombined sources1
Modified residuei927PhosphothreonineCombined sources1
Modified residuei931PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by FER.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiB9EKI3.
MaxQBiB9EKI3.
PaxDbiB9EKI3.
PeptideAtlasiB9EKI3.
PRIDEiB9EKI3.

PTM databases

iPTMnetiB9EKI3.
PhosphoSitePlusiB9EKI3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030059.
ExpressionAtlasiB9EKI3. baseline and differential.

Interactioni

Subunit structurei

Component of the SNF/SWI transcription factor complexes (By similarity). Interacts with RAB6A. Interacts with TCEB1 (By similarity). Interacts with STAT3 and FER. Interacts with TRNP1; may regulate TRNP1 proteasomal degradation.By similarity2 Publications

Protein-protein interaction databases

BioGridi231235. 4 interactors.
IntActiB9EKI3. 1 interactor.
STRINGi10090.ENSMUSP00000093325.

Structurei

3D structure databases

ProteinModelPortaliB9EKI3.
SMRiB9EKI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni329 – 338Interaction with Elongin BC complexBy similarity10

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili443 – 767Sequence analysisAdd BLAST325
Coiled coili824 – 894Sequence analysisAdd BLAST71
Coiled coili984 – 1090Sequence analysisAdd BLAST107

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi235 – 340Ser-richAdd BLAST106
Compositional biasi374 – 377Poly-Glu4

Domaini

The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGA0. Eukaryota.
ENOG410YE9Z. LUCA.
GeneTreeiENSGT00390000010697.
HOGENOMiHOG000154639.
InParanoidiB9EKI3.
KOiK20286.
OMAiASACPEY.
OrthoDBiEOG091G01F7.
TreeFamiTF329420.

Family and domain databases

InterProiIPR022092. TMF_DNA-bd.
IPR022091. TMF_TATA-bd.
[Graphical view]
PfamiPF12329. TMF_DNA_bd. 1 hit.
PF12325. TMF_TATA_bd. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9EKI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWFNASQLS SFAKQALSQA QKSIDRVLDI QEEEPSAWAE AIPYGEPGIS
60 70 80 90 100
PPVSGGWDTS TWGLNSTSSE PQSPPTASQA ITKPVRRTVV DESENFFSAF
110 120 130 140 150
LSPSDAHTIQ KSPVVSKPPS KSQRPEEEVK SSLQESSSPG QSRVSETAEV
160 170 180 190 200
RDSVCVSGET SAVGTPSPVP EDKHEETAGE ESEVKVPTVR LKASENVVNV
210 220 230 240 250
NTTEDVSTTS TQSLTAETKD MALEPKEQKH EDRQSNTPSP PVSSFSSGTS
260 270 280 290 300
TTSDIEVLDH ESVISESSAS SRQETSDAKS SLHLMQTSFQ LLSASACPEY
310 320 330 340 350
SRLDDFQKLN ESCCSSDAFE RIDSFSVQSL DSRSVSEINS DDELPGKGYA
360 370 380 390 400
LVPIIVSPST PKTKVVESTE ENAEEEEGNE TLVAPSEEAE LEESGRSATP
410 420 430 440 450
VNCDQPDILA SPTAGSGGHS ASGPATEQCE AVENQPKAPP EKEDVCKTVE
460 470 480 490 500
FLNEKLEKRE TQLLSLSKEK ALLEEAYDNL KDEMFRVKEE SSSISSLKDE
510 520 530 540 550
FTQRIAEAEK KVQLACKERD AAKKEMKTIK EELATRLNSS QTADLLKEKD
560 570 580 590 600
EQIQGLMEEG EKLSKQQLHN SNIIKKLRAK DKDNENVIAK LNRKAKELEE
610 620 630 640 650
ELQHLRQVLD GKEEVEKQHR ENIKKLNSVV ERQEKDLGRL QVDMDELEEK
660 670 680 690 700
SRSTQAALDS AYRELTDLHK ANAAKDSEVQ EAALRREMKA KEELSGALEK
710 720 730 740 750
AQEEARQQQE ALVLQVGDLR LALQRAEQAA ARKEDYLRHE ISELQQRLQE
760 770 780 790 800
AENRNQELSQ SVSSTARPLL RQIENLQATL GSQTSSWETL EKSLSDRLGE
810 820 830 840 850
SQTLLAAAVE RERAATEELL ANKIQMSSVE SQNTLLRQEN SRLQAQLESE
860 870 880 890 900
KNKLRKLEDE NSRYQVELEN LKDEYVRTLE ESRKEKTLLS SQLEMERMKV
910 920 930 940 950
EQERKKTIFT QEALKEKDHK LFSVCSTPTM SRSSSISGVD AAGLQASFLS
960 970 980 990 1000
QDESHDHSFG PMSTSASGSN LYEAVRMGAG SSIIENLQSQ LKLREGEISH
1010 1020 1030 1040 1050
LQLEISNLEK TRSIMSEELV KLTNQNDELE EKVKEIPKLR VQLRDLDQRY
1060 1070 1080 1090
NTILQMYGEK AEEAEELRLD LEDVKNMYKT QIDELLRQRL S
Length:1,091
Mass (Da):121,803
Last modified:July 27, 2011 - v2
Checksum:iD208A815FF740CF1
GO

Sequence cautioni

The sequence BAE26035 differs from that shown. Reason: Frameshift at position 44.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106A → V in AAI50932 (PubMed:15489334).Curated1
Sequence conflicti144V → A in AAI50932 (PubMed:15489334).Curated1
Sequence conflicti161S → P in AAI50932 (PubMed:15489334).Curated1
Sequence conflicti203T → S in AAI50932 (PubMed:15489334).Curated1
Sequence conflicti439P → L in AAI50932 (PubMed:15489334).Curated1
Sequence conflicti871L → I in AAI50932 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC155724 Genomic DNA. No translation available.
BC150931 mRNA. Translation: AAI50932.1.
AK144898 mRNA. Translation: BAE26120.1.
AK144729 mRNA. Translation: BAE26035.1. Frameshift.
CCDSiCCDS39575.1.
RefSeqiNP_001074580.1. NM_001081111.2.
UniGeneiMm.30893.

Genome annotation databases

EnsembliENSMUST00000095664; ENSMUSP00000093325; ENSMUSG00000030059.
GeneIDi232286.
KEGGimmu:232286.
UCSCiuc009dak.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC155724 Genomic DNA. No translation available.
BC150931 mRNA. Translation: AAI50932.1.
AK144898 mRNA. Translation: BAE26120.1.
AK144729 mRNA. Translation: BAE26035.1. Frameshift.
CCDSiCCDS39575.1.
RefSeqiNP_001074580.1. NM_001081111.2.
UniGeneiMm.30893.

3D structure databases

ProteinModelPortaliB9EKI3.
SMRiB9EKI3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231235. 4 interactors.
IntActiB9EKI3. 1 interactor.
STRINGi10090.ENSMUSP00000093325.

PTM databases

iPTMnetiB9EKI3.
PhosphoSitePlusiB9EKI3.

Proteomic databases

EPDiB9EKI3.
MaxQBiB9EKI3.
PaxDbiB9EKI3.
PeptideAtlasiB9EKI3.
PRIDEiB9EKI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095664; ENSMUSP00000093325; ENSMUSG00000030059.
GeneIDi232286.
KEGGimmu:232286.
UCSCiuc009dak.2. mouse.

Organism-specific databases

CTDi7110.
MGIiMGI:2684999. Tmf1.

Phylogenomic databases

eggNOGiENOG410IGA0. Eukaryota.
ENOG410YE9Z. LUCA.
GeneTreeiENSGT00390000010697.
HOGENOMiHOG000154639.
InParanoidiB9EKI3.
KOiK20286.
OMAiASACPEY.
OrthoDBiEOG091G01F7.
TreeFamiTF329420.

Enzyme and pathway databases

ReactomeiR-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

PROiB9EKI3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030059.
ExpressionAtlasiB9EKI3. baseline and differential.

Family and domain databases

InterProiIPR022092. TMF_DNA-bd.
IPR022091. TMF_TATA-bd.
[Graphical view]
PfamiPF12329. TMF_DNA_bd. 1 hit.
PF12325. TMF_TATA_bd. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMF1_MOUSE
AccessioniPrimary (citable) accession number: B9EKI3
Secondary accession number(s): E9QPJ3, Q3UMH8, Q3UMR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.