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Protein

TATA element modulatory factor

Gene

Tmf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Potential coactivator of the androgen receptor. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER (By similarity). Mediates STAT3 degradation.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • acrosome assembly Source: MGI
  • cellular response to organic cyclic compound Source: MGI
  • defense response to bacterium Source: MGI
  • Leydig cell differentiation Source: MGI
  • luteinizing hormone secretion Source: MGI
  • male gonad development Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of gene expression Source: MGI
  • positive regulation of cytokine production Source: MGI
  • positive regulation of testosterone secretion Source: MGI
  • regulation of proteasomal protein catabolic process Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • spermatid development Source: MGI
  • spermatid nucleus differentiation Source: MGI
  • sperm motility Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA element modulatory factor
Short name:
TMF
Alternative name(s):
Androgen receptor coactivator 160 kDa protein
Androgen receptor-associated protein of 160 kDa
Gene namesi
Name:Tmf1
Synonyms:Ara160, Gm153
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2684999. Tmf1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10911091TATA element modulatory factorPRO_0000376797Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei73 – 731PhosphoserineBy similarity
Modified residuei78 – 781PhosphoserineBy similarity
Modified residuei112 – 1121PhosphoserineBy similarity
Modified residuei136 – 1361PhosphoserineBy similarity
Modified residuei213 – 2131PhosphoserineBy similarity
Modified residuei324 – 3241PhosphoserineCombined sources
Modified residuei326 – 3261PhosphoserineCombined sources
Modified residuei329 – 3291PhosphoserineCombined sources
Modified residuei334 – 3341PhosphoserineBy similarity
Modified residuei340 – 3401PhosphoserineCombined sources
Modified residuei357 – 3571PhosphoserineCombined sources
Modified residuei411 – 4111PhosphoserineCombined sources
Modified residuei540 – 5401PhosphoserineBy similarity
Modified residuei923 – 9231PhosphoserineBy similarity
Modified residuei926 – 9261PhosphoserineCombined sources
Modified residuei927 – 9271PhosphothreonineCombined sources
Modified residuei931 – 9311PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated by FER.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiB9EKI3.
MaxQBiB9EKI3.
PaxDbiB9EKI3.
PeptideAtlasiB9EKI3.
PRIDEiB9EKI3.

PTM databases

iPTMnetiB9EKI3.

Expressioni

Gene expression databases

BgeeiB9EKI3.
ExpressionAtlasiB9EKI3. baseline and differential.

Interactioni

Subunit structurei

Component of the SNF/SWI transcription factor complexes (By similarity). Interacts with RAB6A. Interacts with TCEB1 (By similarity). Interacts with STAT3 and FER. Interacts with TRNP1; may regulate TRNP1 proteasomal degradation.By similarity2 Publications

Protein-protein interaction databases

BioGridi231235. 4 interactions.
IntActiB9EKI3. 1 interaction.
STRINGi10090.ENSMUSP00000093325.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni329 – 33810Interaction with Elongin BC complexBy similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili443 – 767325Sequence analysisAdd
BLAST
Coiled coili824 – 89471Sequence analysisAdd
BLAST
Coiled coili984 – 1090107Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi235 – 340106Ser-richAdd
BLAST
Compositional biasi374 – 3774Poly-Glu

Domaini

The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGA0. Eukaryota.
ENOG410YE9Z. LUCA.
GeneTreeiENSGT00390000010697.
HOGENOMiHOG000154639.
InParanoidiB9EKI3.
OMAiSWFNASQ.
OrthoDBiEOG7X0VHV.
TreeFamiTF329420.

Family and domain databases

InterProiIPR022092. TMF_DNA-bd.
IPR022091. TMF_TATA-bd.
[Graphical view]
PfamiPF12329. TMF_DNA_bd. 1 hit.
PF12325. TMF_TATA_bd. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9EKI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWFNASQLS SFAKQALSQA QKSIDRVLDI QEEEPSAWAE AIPYGEPGIS
60 70 80 90 100
PPVSGGWDTS TWGLNSTSSE PQSPPTASQA ITKPVRRTVV DESENFFSAF
110 120 130 140 150
LSPSDAHTIQ KSPVVSKPPS KSQRPEEEVK SSLQESSSPG QSRVSETAEV
160 170 180 190 200
RDSVCVSGET SAVGTPSPVP EDKHEETAGE ESEVKVPTVR LKASENVVNV
210 220 230 240 250
NTTEDVSTTS TQSLTAETKD MALEPKEQKH EDRQSNTPSP PVSSFSSGTS
260 270 280 290 300
TTSDIEVLDH ESVISESSAS SRQETSDAKS SLHLMQTSFQ LLSASACPEY
310 320 330 340 350
SRLDDFQKLN ESCCSSDAFE RIDSFSVQSL DSRSVSEINS DDELPGKGYA
360 370 380 390 400
LVPIIVSPST PKTKVVESTE ENAEEEEGNE TLVAPSEEAE LEESGRSATP
410 420 430 440 450
VNCDQPDILA SPTAGSGGHS ASGPATEQCE AVENQPKAPP EKEDVCKTVE
460 470 480 490 500
FLNEKLEKRE TQLLSLSKEK ALLEEAYDNL KDEMFRVKEE SSSISSLKDE
510 520 530 540 550
FTQRIAEAEK KVQLACKERD AAKKEMKTIK EELATRLNSS QTADLLKEKD
560 570 580 590 600
EQIQGLMEEG EKLSKQQLHN SNIIKKLRAK DKDNENVIAK LNRKAKELEE
610 620 630 640 650
ELQHLRQVLD GKEEVEKQHR ENIKKLNSVV ERQEKDLGRL QVDMDELEEK
660 670 680 690 700
SRSTQAALDS AYRELTDLHK ANAAKDSEVQ EAALRREMKA KEELSGALEK
710 720 730 740 750
AQEEARQQQE ALVLQVGDLR LALQRAEQAA ARKEDYLRHE ISELQQRLQE
760 770 780 790 800
AENRNQELSQ SVSSTARPLL RQIENLQATL GSQTSSWETL EKSLSDRLGE
810 820 830 840 850
SQTLLAAAVE RERAATEELL ANKIQMSSVE SQNTLLRQEN SRLQAQLESE
860 870 880 890 900
KNKLRKLEDE NSRYQVELEN LKDEYVRTLE ESRKEKTLLS SQLEMERMKV
910 920 930 940 950
EQERKKTIFT QEALKEKDHK LFSVCSTPTM SRSSSISGVD AAGLQASFLS
960 970 980 990 1000
QDESHDHSFG PMSTSASGSN LYEAVRMGAG SSIIENLQSQ LKLREGEISH
1010 1020 1030 1040 1050
LQLEISNLEK TRSIMSEELV KLTNQNDELE EKVKEIPKLR VQLRDLDQRY
1060 1070 1080 1090
NTILQMYGEK AEEAEELRLD LEDVKNMYKT QIDELLRQRL S
Length:1,091
Mass (Da):121,803
Last modified:July 27, 2011 - v2
Checksum:iD208A815FF740CF1
GO

Sequence cautioni

The sequence BAE26035.1 differs from that shown. Reason: Frameshift at position 44. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061A → V in AAI50932 (PubMed:15489334).Curated
Sequence conflicti144 – 1441V → A in AAI50932 (PubMed:15489334).Curated
Sequence conflicti161 – 1611S → P in AAI50932 (PubMed:15489334).Curated
Sequence conflicti203 – 2031T → S in AAI50932 (PubMed:15489334).Curated
Sequence conflicti439 – 4391P → L in AAI50932 (PubMed:15489334).Curated
Sequence conflicti871 – 8711L → I in AAI50932 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC155724 Genomic DNA. No translation available.
BC150931 mRNA. Translation: AAI50932.1.
AK144898 mRNA. Translation: BAE26120.1.
AK144729 mRNA. Translation: BAE26035.1. Frameshift.
CCDSiCCDS39575.1.
RefSeqiNP_001074580.1. NM_001081111.2.
UniGeneiMm.30893.

Genome annotation databases

EnsembliENSMUST00000095664; ENSMUSP00000093325; ENSMUSG00000030059.
GeneIDi232286.
KEGGimmu:232286.
UCSCiuc009dak.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC155724 Genomic DNA. No translation available.
BC150931 mRNA. Translation: AAI50932.1.
AK144898 mRNA. Translation: BAE26120.1.
AK144729 mRNA. Translation: BAE26035.1. Frameshift.
CCDSiCCDS39575.1.
RefSeqiNP_001074580.1. NM_001081111.2.
UniGeneiMm.30893.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231235. 4 interactions.
IntActiB9EKI3. 1 interaction.
STRINGi10090.ENSMUSP00000093325.

PTM databases

iPTMnetiB9EKI3.

Proteomic databases

EPDiB9EKI3.
MaxQBiB9EKI3.
PaxDbiB9EKI3.
PeptideAtlasiB9EKI3.
PRIDEiB9EKI3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095664; ENSMUSP00000093325; ENSMUSG00000030059.
GeneIDi232286.
KEGGimmu:232286.
UCSCiuc009dak.2. mouse.

Organism-specific databases

CTDi7110.
MGIiMGI:2684999. Tmf1.

Phylogenomic databases

eggNOGiENOG410IGA0. Eukaryota.
ENOG410YE9Z. LUCA.
GeneTreeiENSGT00390000010697.
HOGENOMiHOG000154639.
InParanoidiB9EKI3.
OMAiSWFNASQ.
OrthoDBiEOG7X0VHV.
TreeFamiTF329420.

Enzyme and pathway databases

ReactomeiR-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

PROiB9EKI3.
SOURCEiSearch...

Gene expression databases

BgeeiB9EKI3.
ExpressionAtlasiB9EKI3. baseline and differential.

Family and domain databases

InterProiIPR022092. TMF_DNA-bd.
IPR022091. TMF_TATA-bd.
[Graphical view]
PfamiPF12329. TMF_DNA_bd. 1 hit.
PF12325. TMF_TATA_bd. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-619.
    Tissue: Lung.
  4. "Tyrosine phosphorylation of the TATA element modulatory factor by the FER nuclear tyrosine kinases."
    Schwartz Y., Ben-Dor I., Navon A., Motro B., Nir U.
    FEBS Lett. 434:339-345(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY FER.
  5. "TMF/ARA160 is a BC-box-containing protein that mediates the degradation of Stat3."
    Perry E., Tsruya R., Levitsky P., Pomp O., Taller M., Weisberg S., Parris W., Kulkarni S., Malovani H., Pawson T., Shpungin S., Nir U.
    Oncogene 23:8908-8919(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH FER AND STAT3.
  6. "TRNP: a conserved mammalian gene encoding a nuclear protein that accelerates cell-cycle progression."
    Volpe M., Shpungin S., Barbi C., Abrham G., Malovani H., Wides R., Nir U.
    DNA Cell Biol. 25:331-339(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRNP1.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-324; SER-326; SER-329; SER-340; SER-357; SER-411; SER-926; THR-927 AND SER-931, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiTMF1_MOUSE
AccessioniPrimary (citable) accession number: B9EKI3
Secondary accession number(s): E9QPJ3, Q3UMH8, Q3UMR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.