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Protein

CAP-Gly domain-containing linker protein 3

Gene

Clip3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics. May modulate the cellular compartmentalization of AKT kinase family and promote its cell membrane localization, thereby playing a role in glucose transport in adipocytes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-5357905. Regulation of TNFR1 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
CAP-Gly domain-containing linker protein 3
Alternative name(s):
Cytoplasmic linker protein 170-related 59 kDa protein
Short name:
CLIP-170-related 59 kDa protein
Short name:
CLIPR-59
Gene namesi
Name:Clip3
Synonyms:Clipr59
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1923936. Clip3.

Subcellular locationi

GO - Cellular componenti

  • early endosome membrane Source: UniProtKB
  • Golgi stack Source: UniProtKB-SubCell
  • membrane raft Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • recycling endosome membrane Source: UniProtKB
  • trans-Golgi network Source: UniProtKB
  • trans-Golgi network membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004156701 – 547CAP-Gly domain-containing linker protein 3Add BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei374PhosphothreonineCombined sources1
Modified residuei399PhosphoserineCombined sources1
Modified residuei401PhosphoserineCombined sources1
Lipidationi534S-palmitoyl cysteineBy similarity1
Lipidationi535S-palmitoyl cysteineBy similarity1

Post-translational modificationi

Palmitoylation by ZDHHC17 regulates association with the plasma membrane.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiB9EHT4.
PaxDbiB9EHT4.
PeptideAtlasiB9EHT4.
PRIDEiB9EHT4.

PTM databases

iPTMnetiB9EHT4.
PhosphoSitePlusiB9EHT4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000013921.
ExpressionAtlasiB9EHT4. baseline and differential.
GenevisibleiB9EHT4. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with AKT1 and AKT2; when AKT1 and AKT2 are phosphorylated and activated, affinity is higher for AKT2 (By similarity). Interacts with ZDHHC13 (via ANK repeats) (PubMed:26198635). Interacts with ZDHHC17 (via ANK repeats) (PubMed:26198635).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi218260. 2 interactors.
STRINGi10090.ENSMUSP00000014065.

Structurei

Secondary structure

1547
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi406 – 409Combined sources4
Beta strandi420 – 424Combined sources5
Turni425 – 427Combined sources3
Beta strandi428 – 437Combined sources10
Beta strandi439 – 449Combined sources11
Beta strandi451 – 453Combined sources3
Beta strandi456 – 461Combined sources6
Turni471 – 473Combined sources3
Beta strandi474 – 477Combined sources4
Helixi479 – 481Combined sources3
Beta strandi482 – 484Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WHHNMR-A399-486[»]
ProteinModelPortaliB9EHT4.
SMRiB9EHT4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati117 – 158ANK 1Add BLAST42
Repeati160 – 191ANK 2Add BLAST32
Repeati197 – 229ANK 3Add BLAST33
Domaini314 – 356CAP-Gly 1PROSITE-ProRule annotationAdd BLAST43
Domaini436 – 478CAP-Gly 2PROSITE-ProRule annotationAdd BLAST43

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni488 – 547GoLDAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi16 – 25Poly-Glu10

Domaini

Microtubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD).By similarity

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 2 CAP-Gly domains.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG4568. Eukaryota.
COG5244. LUCA.
GeneTreeiENSGT00760000119173.
HOGENOMiHOG000007206.
HOVERGENiHBG057079.
InParanoidiB9EHT4.
KOiK10423.
OMAiDNVGAKK.
OrthoDBiEOG091G04NK.
PhylomeDBiB9EHT4.
TreeFamiTF326096.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.30.30.190. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000938. CAP-Gly_domain.
IPR030504. CLIP3.
[Graphical view]
PANTHERiPTHR18916:SF11. PTHR18916:SF11. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF01302. CAP_GLY. 2 hits.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM01052. CAP_GLY. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF74924. SSF74924. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9EHT4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKTDPAPMA PPPRGEEEEE EEEDEPVPEA PSPTQERRQK PVVHPSAPAP
60 70 80 90 100
LPKDYAFTFF DPNDPACQEI LFDPKTTIPE LFAIVRQWVP QVQHKIDVIG
110 120 130 140 150
NEILRRGCHV NDRDGLTDMT LLHYACKAGA HGVGDPAAAV RLSQQLLALG
160 170 180 190 200
ADVTLRSRWT NMNALHYAAY FDVPDLVRVL LKGARPRVVN STCSDFNHGS
210 220 230 240 250
ALHIAASNLC LGAAKCLLEH GANPALRNRK GQVPAEVVPD PMDMSLDKAE
260 270 280 290 300
AALVAKELRT LLEEAVPLSC TLPKVTLPNY DNVPGNLMLS ALGLRLGDRV
310 320 330 340 350
LLDGQKTGTL RFCGTTEFAS GQWVGVELDE PEGKNDGSVG GVRYFICPPK
360 370 380 390 400
QGLFASVSKV SKAVDAPPSS VTSTPRTPRM DFSRVTGKGR REHKGKKKSP
410 420 430 440 450
SSPSLGSLQQ REGAKAEVGD QVLVAGQKQG IVRFYGKTDF APGYWYGIEL
460 470 480 490 500
DQPTGKHDGS VFGVRYFTCA PRHGVFAPAS RIQRIGGSTD PPGDSVGAKK
510 520 530 540
VHQVTMTQPK RTFTTVRTPK DIASENSISR LLFCCWFPWM LRAEMQS
Length:547
Mass (Da):59,587
Last modified:March 24, 2009 - v1
Checksum:i851B8B94DA20A1AD
GO

Sequence cautioni

The sequence BAB23857 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC149067 Genomic DNA. No translation available.
CH466593 Genomic DNA. Translation: EDL24034.1.
BC056173 mRNA. Translation: AAH56173.1.
BC138413 mRNA. Translation: AAI38414.1.
AK005167 mRNA. Translation: BAB23857.1. Different initiation.
CCDSiCCDS39880.1.
RefSeqiNP_001074583.1. NM_001081114.1.
XP_006540473.1. XM_006540410.3.
XP_006540474.1. XM_006540411.3.
UniGeneiMm.159258.

Genome annotation databases

EnsembliENSMUST00000014065; ENSMUSP00000014065; ENSMUSG00000013921.
GeneIDi76686.
KEGGimmu:76686.
UCSCiuc009gea.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC149067 Genomic DNA. No translation available.
CH466593 Genomic DNA. Translation: EDL24034.1.
BC056173 mRNA. Translation: AAH56173.1.
BC138413 mRNA. Translation: AAI38414.1.
AK005167 mRNA. Translation: BAB23857.1. Different initiation.
CCDSiCCDS39880.1.
RefSeqiNP_001074583.1. NM_001081114.1.
XP_006540473.1. XM_006540410.3.
XP_006540474.1. XM_006540411.3.
UniGeneiMm.159258.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WHHNMR-A399-486[»]
ProteinModelPortaliB9EHT4.
SMRiB9EHT4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218260. 2 interactors.
STRINGi10090.ENSMUSP00000014065.

PTM databases

iPTMnetiB9EHT4.
PhosphoSitePlusiB9EHT4.

Proteomic databases

MaxQBiB9EHT4.
PaxDbiB9EHT4.
PeptideAtlasiB9EHT4.
PRIDEiB9EHT4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014065; ENSMUSP00000014065; ENSMUSG00000013921.
GeneIDi76686.
KEGGimmu:76686.
UCSCiuc009gea.1. mouse.

Organism-specific databases

CTDi25999.
MGIiMGI:1923936. Clip3.

Phylogenomic databases

eggNOGiKOG4568. Eukaryota.
COG5244. LUCA.
GeneTreeiENSGT00760000119173.
HOGENOMiHOG000007206.
HOVERGENiHBG057079.
InParanoidiB9EHT4.
KOiK10423.
OMAiDNVGAKK.
OrthoDBiEOG091G04NK.
PhylomeDBiB9EHT4.
TreeFamiTF326096.

Enzyme and pathway databases

ReactomeiR-MMU-5357905. Regulation of TNFR1 signaling.

Miscellaneous databases

ChiTaRSiClip3. mouse.
PROiB9EHT4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000013921.
ExpressionAtlasiB9EHT4. baseline and differential.
GenevisibleiB9EHT4. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
2.30.30.190. 2 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000938. CAP-Gly_domain.
IPR030504. CLIP3.
[Graphical view]
PANTHERiPTHR18916:SF11. PTHR18916:SF11. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF01302. CAP_GLY. 2 hits.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM01052. CAP_GLY. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF74924. SSF74924. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS00845. CAP_GLY_1. 2 hits.
PS50245. CAP_GLY_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLIP3_MOUSE
AccessioniPrimary (citable) accession number: B9EHT4
Secondary accession number(s): Q7TNI1, Q9DB67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: March 24, 2009
Last modified: November 2, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The N-terminal half is dispensable for proper Golgi targeting, whereas the GoLD region is required.By similarity

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.