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B9EB60

- SYI_MACCJ

UniProt

B9EB60 - SYI_MACCJ

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Macrococcus caseolyticus (strain JCSC5402)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
    Binding sitei598 – 5981ATPUniRule annotation
    Metal bindingi883 – 8831ZincUniRule annotation
    Metal bindingi886 – 8861ZincUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi907 – 9071ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMCAS458233:GI03-777-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MCCL_0764
    OrganismiMacrococcus caseolyticus (strain JCSC5402)
    Taxonomic identifieri458233 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesMacrococcus
    ProteomesiUP000001383: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 914914Isoleucine--tRNA ligasePRO_1000189180Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi458233.MCCL_0764.

    Structurei

    3D structure databases

    ProteinModelPortaliB9EB60.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi595 – 5995"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9EB60-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKDTLLMP KTDFPMRGGL PNKEPQIQAQ WDEKKLYEKI LKKNEGRTPY    50
    ILHDGPPYAN GQIHMGHALN KIIKDMIMRY KAMNGYYAPY VPGWDTHGLP 100
    IETALTKKGV DRKSMSEAEF RELCRAYALE QIELQKADFK RLGVNGDWEN 150
    PYITLQEKFE AEQIRLFGDM AAKGYIYKGK KPVYWSPSSE SSLAEAEIEY 200
    QDKVSPSIYV AFDVLDGKGL VDDDVKFVIW TTTPWTLPAN VAIALNKDLD 250
    YVQVRVNDTS YIVAQALLDN VCDAVGWDKE GVQIEKTFKG ADLEFVKARH 300
    PFIDRESLII LGDHVTTDAG TGCVHTAPGH GEDDFIVGQK YELDVISPVD 350
    GKGVYTSEAG EFEGMYYDKA NKVITEKLEA SGHLLKLDFF KHSYPHDWRT 400
    KKPVIFRATP QWFASINKVR QDILNAIEET EFKVEWGKTR IYNMIRDRGD 450
    WVISRQRVWG VPLPVFYAEN GDIIMDKAVI EHVATLVEKH GTNVWYEREA 500
    TDLLPEGYTH PGSPNGVFTK EKDIMDVWFD SGSSHRGVLE ARPELSFPAD 550
    LYFEGSDQYR GWFNSSITTA VATRGKSPYK KLLSHGFVMD GQGRKMSKSL 600
    GNTVLPEKVV KQMGADIIRL WVMSVDYLAD VRISDDILKQ VSEVYRKIRN 650
    TFKFLLGNVN DFNPATDAVP YAELVEIDQF MLNKLYTFVN NAHKHYDNND 700
    YLFMYQELQN FINVELSNFY LDYGKDILYI EAQDSHVRRS MQTVVYEVLT 750
    SLTKVLAPII PHTADEIWSH TPHVTEESVH LADMPEVQSV DTALIGKWNH 800
    FLEIRDDVLK AIEETRNDKV IGKSLEAAVY IQAKNEEDQA LLKSFDNLHQ 850
    LFITSYAEVV DEPQGTDYNL SNVLVKHAEG EKCERCWNYS TVLTEESHAH 900
    PHVCPRCLSV LESK 914
    Length:914
    Mass (Da):104,435
    Last modified:March 24, 2009 - v1
    Checksum:i614783A59E2A6DAB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009484 Genomic DNA. Translation: BAH17471.1.
    RefSeqiWP_012656672.1. NC_011999.1.
    YP_002560167.1. NC_011999.1.

    Genome annotation databases

    EnsemblBacteriaiBAH17471; BAH17471; MCCL_0764.
    GeneIDi7389929.
    KEGGimcl:MCCL_0764.
    PATRICi22423841. VBIMacCas48391_0863.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009484 Genomic DNA. Translation: BAH17471.1 .
    RefSeqi WP_012656672.1. NC_011999.1.
    YP_002560167.1. NC_011999.1.

    3D structure databases

    ProteinModelPortali B9EB60.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 458233.MCCL_0764.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAH17471 ; BAH17471 ; MCCL_0764 .
    GeneIDi 7389929.
    KEGGi mcl:MCCL_0764.
    PATRICi 22423841. VBIMacCas48391_0863.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MCAS458233:GI03-777-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, reflecting the ancestral genome of the human-pathogenic staphylococci."
      Baba T., Kuwahara-Arai K., Uchiyama I., Takeuchi F., Ito T., Hiramatsu K.
      J. Bacteriol. 191:1180-1190(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCSC5402.

    Entry informationi

    Entry nameiSYI_MACCJ
    AccessioniPrimary (citable) accession number: B9EB60
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3