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B9EB60

- SYI_MACCJ

UniProt

B9EB60 - SYI_MACCJ

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Macrococcus caseolyticus (strain JCSC5402)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation
Metal bindingi883 – 8831ZincUniRule annotation
Metal bindingi886 – 8861ZincUniRule annotation
Metal bindingi904 – 9041ZincUniRule annotation
Metal bindingi907 – 9071ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMCAS458233:GI03-777-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MCCL_0764
OrganismiMacrococcus caseolyticus (strain JCSC5402)
Taxonomic identifieri458233 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesMacrococcus
ProteomesiUP000001383: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 914914Isoleucine--tRNA ligasePRO_1000189180Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi458233.MCCL_0764.

Structurei

3D structure databases

ProteinModelPortaliB9EB60.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9EB60-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKDTLLMP KTDFPMRGGL PNKEPQIQAQ WDEKKLYEKI LKKNEGRTPY
60 70 80 90 100
ILHDGPPYAN GQIHMGHALN KIIKDMIMRY KAMNGYYAPY VPGWDTHGLP
110 120 130 140 150
IETALTKKGV DRKSMSEAEF RELCRAYALE QIELQKADFK RLGVNGDWEN
160 170 180 190 200
PYITLQEKFE AEQIRLFGDM AAKGYIYKGK KPVYWSPSSE SSLAEAEIEY
210 220 230 240 250
QDKVSPSIYV AFDVLDGKGL VDDDVKFVIW TTTPWTLPAN VAIALNKDLD
260 270 280 290 300
YVQVRVNDTS YIVAQALLDN VCDAVGWDKE GVQIEKTFKG ADLEFVKARH
310 320 330 340 350
PFIDRESLII LGDHVTTDAG TGCVHTAPGH GEDDFIVGQK YELDVISPVD
360 370 380 390 400
GKGVYTSEAG EFEGMYYDKA NKVITEKLEA SGHLLKLDFF KHSYPHDWRT
410 420 430 440 450
KKPVIFRATP QWFASINKVR QDILNAIEET EFKVEWGKTR IYNMIRDRGD
460 470 480 490 500
WVISRQRVWG VPLPVFYAEN GDIIMDKAVI EHVATLVEKH GTNVWYEREA
510 520 530 540 550
TDLLPEGYTH PGSPNGVFTK EKDIMDVWFD SGSSHRGVLE ARPELSFPAD
560 570 580 590 600
LYFEGSDQYR GWFNSSITTA VATRGKSPYK KLLSHGFVMD GQGRKMSKSL
610 620 630 640 650
GNTVLPEKVV KQMGADIIRL WVMSVDYLAD VRISDDILKQ VSEVYRKIRN
660 670 680 690 700
TFKFLLGNVN DFNPATDAVP YAELVEIDQF MLNKLYTFVN NAHKHYDNND
710 720 730 740 750
YLFMYQELQN FINVELSNFY LDYGKDILYI EAQDSHVRRS MQTVVYEVLT
760 770 780 790 800
SLTKVLAPII PHTADEIWSH TPHVTEESVH LADMPEVQSV DTALIGKWNH
810 820 830 840 850
FLEIRDDVLK AIEETRNDKV IGKSLEAAVY IQAKNEEDQA LLKSFDNLHQ
860 870 880 890 900
LFITSYAEVV DEPQGTDYNL SNVLVKHAEG EKCERCWNYS TVLTEESHAH
910
PHVCPRCLSV LESK
Length:914
Mass (Da):104,435
Last modified:March 24, 2009 - v1
Checksum:i614783A59E2A6DAB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009484 Genomic DNA. Translation: BAH17471.1.
RefSeqiYP_002560167.1. NC_011999.1.

Genome annotation databases

EnsemblBacteriaiBAH17471; BAH17471; MCCL_0764.
GeneIDi7389929.
KEGGimcl:MCCL_0764.
PATRICi22423841. VBIMacCas48391_0863.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009484 Genomic DNA. Translation: BAH17471.1 .
RefSeqi YP_002560167.1. NC_011999.1.

3D structure databases

ProteinModelPortali B9EB60.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 458233.MCCL_0764.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAH17471 ; BAH17471 ; MCCL_0764 .
GeneIDi 7389929.
KEGGi mcl:MCCL_0764.
PATRICi 22423841. VBIMacCas48391_0863.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MCAS458233:GI03-777-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, reflecting the ancestral genome of the human-pathogenic staphylococci."
    Baba T., Kuwahara-Arai K., Uchiyama I., Takeuchi F., Ito T., Hiramatsu K.
    J. Bacteriol. 191:1180-1190(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCSC5402.

Entry informationi

Entry nameiSYI_MACCJ
AccessioniPrimary (citable) accession number: B9EB60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: October 29, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3