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B9EB04

- DEF_MACCJ

UniProt

B9EB04 - DEF_MACCJ

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Protein

Peptide deformylase

Gene

def

Organism
Macrococcus caseolyticus (strain JCSC5402)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe(2+) ion.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi111 – 1111IronUniRule annotation
Metal bindingi154 – 1541IronUniRule annotation
Active sitei155 – 1551UniRule annotation
Metal bindingi158 – 1581IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMCAS458233:GI03-721-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
Short name:
PDFUniRule annotation
Alternative name(s):
Polypeptide deformylaseUniRule annotation
Gene namesi
Name:defUniRule annotation
Ordered Locus Names:MCCL_0708
OrganismiMacrococcus caseolyticus (strain JCSC5402)
Taxonomic identifieri458233 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesMacrococcus
ProteomesiUP000001383: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 184184Peptide deformylasePRO_1000200738Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi458233.MCCL_0708.

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243507.
KOiK01462.
OMAiHIDKENP.
OrthoDBiEOG6PZXGQ.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

B9EB04-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLTMKDIIRD GHPTLRAKAE EVPLPLSTED RQLIDDMLEF LKMSQDEEQS
60 70 80 90 100
RKYQLRSGVG IAAPQLNHKK RMLVIHFYDE KKGDYVTHQL INPKIISHSV
110 120 130 140 150
EKSYLPTGEG CLSVDEAVPG IVHRYARITV KAYTPDGEEV KLRLKDFSAI
160 170 180
VAQHEIDHLN GVMFYDHIDK ADPMKVQKDA IAVE
Length:184
Mass (Da):20,992
Last modified:March 24, 2009 - v1
Checksum:i40B4E4134E13C131
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009484 Genomic DNA. Translation: BAH17415.1.
RefSeqiYP_002560111.1. NC_011999.1.

Genome annotation databases

EnsemblBacteriaiBAH17415; BAH17415; MCCL_0708.
GeneIDi7389894.
KEGGimcl:MCCL_0708.
PATRICi22423719. VBIMacCas48391_0802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009484 Genomic DNA. Translation: BAH17415.1 .
RefSeqi YP_002560111.1. NC_011999.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 458233.MCCL_0708.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAH17415 ; BAH17415 ; MCCL_0708 .
GeneIDi 7389894.
KEGGi mcl:MCCL_0708.
PATRICi 22423719. VBIMacCas48391_0802.

Phylogenomic databases

eggNOGi COG0242.
HOGENOMi HOG000243507.
KOi K01462.
OMAi HIDKENP.
OrthoDBi EOG6PZXGQ.

Enzyme and pathway databases

BioCyci MCAS458233:GI03-721-MONOMER.

Family and domain databases

Gene3Di 3.90.45.10. 1 hit.
HAMAPi MF_00163. Pep_deformylase.
InterProi IPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view ]
PANTHERi PTHR10458. PTHR10458. 1 hit.
Pfami PF01327. Pep_deformylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF004749. Pep_def. 1 hit.
PRINTSi PR01576. PDEFORMYLASE.
SUPFAMi SSF56420. SSF56420. 1 hit.
TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, reflecting the ancestral genome of the human-pathogenic staphylococci."
    Baba T., Kuwahara-Arai K., Uchiyama I., Takeuchi F., Ito T., Hiramatsu K.
    J. Bacteriol. 191:1180-1190(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCSC5402.

Entry informationi

Entry nameiDEF_MACCJ
AccessioniPrimary (citable) accession number: B9EB04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3