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B9EA22 (BIOB_MACCJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:MCCL_0376
OrganismMacrococcus caseolyticus (strain JCSC5402) [Complete proteome] [HAMAP]
Taxonomic identifier458233 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesMacrococcus

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 330330Biotin synthase HAMAP-Rule MF_01694
PRO_0000381450

Sites

Metal binding601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1031Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1361Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1961Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2661Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B9EA22 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 9DD33AF2868FB87F

FASTA33036,983
        10         20         30         40         50         60 
MLAQRIINGE NISKEEALSL FVDNNIDTYD LLHEAYQVRK YYYGRKVKLN MILNAKSGIC 

        70         80         90        100        110        120 
PEDCGYCGQS KLMKNKDKYL LVDSEQIKSG ADFCATHDIG TYCIVMSGRG PSDKEVEHIA 

       130        140        150        160        170        180 
DTVEAIKHEH PQLKICACLG LTNDEQAKKL KLAGVDRYNH NINTSENYHD EVVTTHTYQD 

       190        200        210        220        230        240 
RVDTIEIMKA NNISPCSGVI CGMGETDEDI IDMALALRAI DADSIPVNFL HPVKGTKFGN 

       250        260        270        280        290        300 
EDNLTPERCL RILSMFRLIN PSKEIRIAGG REVNLRTLQP LAMQAANSIF VGDYLITDGQ 

       310        320        330 
PNELDYKMLE DLGFEIDINE SEINKAALKI 

« Hide

References

[1]"Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, reflecting the ancestral genome of the human-pathogenic staphylococci."
Baba T., Kuwahara-Arai K., Uchiyama I., Takeuchi F., Ito T., Hiramatsu K.
J. Bacteriol. 191:1180-1190(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCSC5402.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009484 Genomic DNA. Translation: BAH17083.1.
RefSeqYP_002559779.1. NC_011999.1.

3D structure databases

ProteinModelPortalB9EA22.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING458233.MCCL_0376.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAH17083; BAH17083; MCCL_0376.
GeneID7391074.
KEGGmcl:MCCL_0376.
PATRIC22423036. VBIMacCas48391_0461.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.
OrthoDBEOG622PMP.
ProtClustDBPRK06256.

Enzyme and pathway databases

BioCycMCAS458233:GI03-389-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_MACCJ
AccessionPrimary (citable) accession number: B9EA22
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways