SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B9EA22

- BIOB_MACCJ

UniProt

B9EA22 - BIOB_MACCJ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Biotin synthase
Gene
bioB, MCCL_0376
Organism
Macrococcus caseolyticus (strain JCSC5402)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi103 – 1031Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi136 – 1361Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi196 – 1961Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi266 – 2661Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMCAS458233:GI03-389-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:MCCL_0376
OrganismiMacrococcus caseolyticus (strain JCSC5402)
Taxonomic identifieri458233 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesMacrococcus
ProteomesiUP000001383: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Biotin synthaseUniRule annotation
PRO_0000381450Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi458233.MCCL_0376.

Structurei

3D structure databases

ProteinModelPortaliB9EA22.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiTCENTLR.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B9EA22-1 [UniParc]FASTAAdd to Basket

« Hide

MLAQRIINGE NISKEEALSL FVDNNIDTYD LLHEAYQVRK YYYGRKVKLN    50
MILNAKSGIC PEDCGYCGQS KLMKNKDKYL LVDSEQIKSG ADFCATHDIG 100
TYCIVMSGRG PSDKEVEHIA DTVEAIKHEH PQLKICACLG LTNDEQAKKL 150
KLAGVDRYNH NINTSENYHD EVVTTHTYQD RVDTIEIMKA NNISPCSGVI 200
CGMGETDEDI IDMALALRAI DADSIPVNFL HPVKGTKFGN EDNLTPERCL 250
RILSMFRLIN PSKEIRIAGG REVNLRTLQP LAMQAANSIF VGDYLITDGQ 300
PNELDYKMLE DLGFEIDINE SEINKAALKI 330
Length:330
Mass (Da):36,983
Last modified:March 24, 2009 - v1
Checksum:i9DD33AF2868FB87F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009484 Genomic DNA. Translation: BAH17083.1.
RefSeqiYP_002559779.1. NC_011999.1.

Genome annotation databases

EnsemblBacteriaiBAH17083; BAH17083; MCCL_0376.
GeneIDi7391074.
KEGGimcl:MCCL_0376.
PATRICi22423036. VBIMacCas48391_0461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP009484 Genomic DNA. Translation: BAH17083.1 .
RefSeqi YP_002559779.1. NC_011999.1.

3D structure databases

ProteinModelPortali B9EA22.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 458233.MCCL_0376.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAH17083 ; BAH17083 ; MCCL_0376 .
GeneIDi 7391074.
KEGGi mcl:MCCL_0376.
PATRICi 22423036. VBIMacCas48391_0461.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi TCENTLR.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci MCAS458233:GI03-389-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, reflecting the ancestral genome of the human-pathogenic staphylococci."
    Baba T., Kuwahara-Arai K., Uchiyama I., Takeuchi F., Ito T., Hiramatsu K.
    J. Bacteriol. 191:1180-1190(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCSC5402.

Entry informationi

Entry nameiBIOB_MACCJ
AccessioniPrimary (citable) accession number: B9EA22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi