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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Macrococcus caseolyticus (strain JCSC5402)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei577 – 5771ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMCAS458233:GI03-1448-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:MCCL_1435
OrganismiMacrococcus caseolyticus (strain JCSC5402)
Taxonomic identifieri458233 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesMacrococcus
ProteomesiUP000001383: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 802802Leucine--tRNA ligasePRO_1000199213Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi458233.MCCL_1435.

Structurei

3D structure databases

ProteinModelPortaliB9E7H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionAdd
BLAST
Motifi574 – 5785"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9E7H4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFNHQTIEK KWQKYWLDNH TFKTTEDKDK NFYALDMFPY PSGAGLHVGH
60 70 80 90 100
PEGYTATDII SRFKRMQGYN VLHPMGWDAF GLPAEQYAID TGNDPAEFTE
110 120 130 140 150
KNIATFKRQI QELGFSYDWE REINTTDPEY YKWTQWIFIQ LYKKGLAYVD
160 170 180 190 200
EVAVNWCPAL GTVLSNEEVI DGVSERGGHP VIRKPMRQWV LKITEYADRL
210 220 230 240 250
LEDLEELDWP ESLKDMQRNW IGRSEGAEVA FEVENLDHSF KVFTTRPDTI
260 270 280 290 300
YGATYAVLSP EHELVAAITS EEQAEAVKAY QEQAARKSDL ERTDLAKDKT
310 320 330 340 350
GVFTGSYAIN PFNGERMPIW ISDYVLASYG TGAIMAVPAH DERDFEFAKQ
360 370 380 390 400
FGLDIKPVIE GGDNTSAYTG DGAHINSGEL DGLNKEEGIR RAIELLEAKG
410 420 430 440 450
IGEKKVSYKL RDWLFSRQRY WGEPIPVITW EDGSMTTVPE EELPLMLPKT
460 470 480 490 500
EHIKPSGTGE SPLANIDEFV NVTDPVTGMK GRRETNTMPQ WAGSCWYYLR
510 520 530 540 550
YIDPKNSDMI ADPELLKKWL PVDLYIGGAE HAVLHLLYAR FWHKVLYDLG
560 570 580 590 600
VVHTKEPFQK LFNQGMILGE GNEKMSKSKG NVVNPDDVVA SHGADTLRLY
610 620 630 640 650
EMFMGPLDAS IAWSTNGLDG ARRFLDRVYR LLVNEDGTLS EKITEQPVGS
660 670 680 690 700
MEKVYHQTVK KVTDDYETLG FNTAISQMMV FINEGYKSEQ LNIDHIRGFV
710 720 730 740 750
KLLNPIAPHI TEELWEKLGG TESITYEAWP VYDESKLVDS EVEIVIQVNG
760 770 780 790 800
KLKQKATIAK DMDKSEMETF ALSLEAVQTA IEGKTVRKVI AVPNKLVNIV

AN
Length:802
Mass (Da):91,101
Last modified:March 24, 2009 - v1
Checksum:i456BBFFEAA2D2A37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009484 Genomic DNA. Translation: BAH18142.1.
RefSeqiWP_012657340.1. NC_011999.1.
YP_002560838.1. NC_011999.1.

Genome annotation databases

EnsemblBacteriaiBAH18142; BAH18142; MCCL_1435.
GeneIDi7389403.
KEGGimcl:MCCL_1435.
PATRICi22425257. VBIMacCas48391_1569.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009484 Genomic DNA. Translation: BAH18142.1.
RefSeqiWP_012657340.1. NC_011999.1.
YP_002560838.1. NC_011999.1.

3D structure databases

ProteinModelPortaliB9E7H4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi458233.MCCL_1435.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH18142; BAH18142; MCCL_1435.
GeneIDi7389403.
KEGGimcl:MCCL_1435.
PATRICi22425257. VBIMacCas48391_1569.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciMCAS458233:GI03-1448-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, reflecting the ancestral genome of the human-pathogenic staphylococci."
    Baba T., Kuwahara-Arai K., Uchiyama I., Takeuchi F., Ito T., Hiramatsu K.
    J. Bacteriol. 191:1180-1190(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCSC5402.

Entry informationi

Entry nameiSYL_MACCJ
AccessioniPrimary (citable) accession number: B9E7H4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: January 7, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.