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B9E7H4 (SYL_MACCJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MCCL_1435
OrganismMacrococcus caseolyticus (strain JCSC5402) [Complete proteome] [HAMAP]
Taxonomic identifier458233 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesMacrococcus

Protein attributes

Sequence length802 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 802802Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199213

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif574 – 5785"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5771ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B9E7H4 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 456BBFFEAA2D2A37

FASTA80291,101
        10         20         30         40         50         60 
MSFNHQTIEK KWQKYWLDNH TFKTTEDKDK NFYALDMFPY PSGAGLHVGH PEGYTATDII 

        70         80         90        100        110        120 
SRFKRMQGYN VLHPMGWDAF GLPAEQYAID TGNDPAEFTE KNIATFKRQI QELGFSYDWE 

       130        140        150        160        170        180 
REINTTDPEY YKWTQWIFIQ LYKKGLAYVD EVAVNWCPAL GTVLSNEEVI DGVSERGGHP 

       190        200        210        220        230        240 
VIRKPMRQWV LKITEYADRL LEDLEELDWP ESLKDMQRNW IGRSEGAEVA FEVENLDHSF 

       250        260        270        280        290        300 
KVFTTRPDTI YGATYAVLSP EHELVAAITS EEQAEAVKAY QEQAARKSDL ERTDLAKDKT 

       310        320        330        340        350        360 
GVFTGSYAIN PFNGERMPIW ISDYVLASYG TGAIMAVPAH DERDFEFAKQ FGLDIKPVIE 

       370        380        390        400        410        420 
GGDNTSAYTG DGAHINSGEL DGLNKEEGIR RAIELLEAKG IGEKKVSYKL RDWLFSRQRY 

       430        440        450        460        470        480 
WGEPIPVITW EDGSMTTVPE EELPLMLPKT EHIKPSGTGE SPLANIDEFV NVTDPVTGMK 

       490        500        510        520        530        540 
GRRETNTMPQ WAGSCWYYLR YIDPKNSDMI ADPELLKKWL PVDLYIGGAE HAVLHLLYAR 

       550        560        570        580        590        600 
FWHKVLYDLG VVHTKEPFQK LFNQGMILGE GNEKMSKSKG NVVNPDDVVA SHGADTLRLY 

       610        620        630        640        650        660 
EMFMGPLDAS IAWSTNGLDG ARRFLDRVYR LLVNEDGTLS EKITEQPVGS MEKVYHQTVK 

       670        680        690        700        710        720 
KVTDDYETLG FNTAISQMMV FINEGYKSEQ LNIDHIRGFV KLLNPIAPHI TEELWEKLGG 

       730        740        750        760        770        780 
TESITYEAWP VYDESKLVDS EVEIVIQVNG KLKQKATIAK DMDKSEMETF ALSLEAVQTA 

       790        800 
IEGKTVRKVI AVPNKLVNIV AN 

« Hide

References

[1]"Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, reflecting the ancestral genome of the human-pathogenic staphylococci."
Baba T., Kuwahara-Arai K., Uchiyama I., Takeuchi F., Ito T., Hiramatsu K.
J. Bacteriol. 191:1180-1190(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCSC5402.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009484 Genomic DNA. Translation: BAH18142.1.
RefSeqYP_002560838.1. NC_011999.1.

3D structure databases

ProteinModelPortalB9E7H4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING458233.MCCL_1435.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAH18142; BAH18142; MCCL_1435.
GeneID7389403.
KEGGmcl:MCCL_1435.
PATRIC22425257. VBIMacCas48391_1569.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycMCAS458233:GI03-1448-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MACCJ
AccessionPrimary (citable) accession number: B9E7H4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: June 11, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries