B9E6Y7 (B9E6Y7_MACCJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase HAMAP MF_01684
Short name=MTA/SAH nucleosidase HAMAP MF_01684
Short name=MTAN HAMAP MF_01684
EC=3.2.2.9 HAMAP MF_01684

Alternative name(s):
5'-methylthioadenosine nucleosidase HAMAP MF_01684
S-adenosylhomocysteine nucleosidase HAMAP MF_01684

Gene names

Name:	mtnN HAMAP MF_01684
Ordered Locus Names:	MCCL_1248

Organism

Macrococcus caseolyticus (strain JCSC5402) [Complete proteome] [HAMAP]

Taxonomic identifier

458233 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Macrococcus

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively By similarity. HAMAP MF_01684
Catalytic activity	S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine. HAMAP MF_01684 SAAS SAAS010049 S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine. HAMAP MF_01684 SAAS SAAS010049
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. HAMAP MF_01684
Sequence similarities	Belongs to the PNP/UDP phosphorylase family. MtnN subfamily. HAMAP MF_01684

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis HAMAP MF_01684 SAAS SAAS010049
Molecular function	Hydrolase HAMAP MF_01684 SAAS SAAS010049
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro nucleoside catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenosylhomocysteine nucleosidase activity Inferred from electronic annotation. Source: HAMAP methylthioadenosine nucleosidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	177 – 178	2	Substrate binding By similarity HAMAP MF_01684
Sites
	Active site	16	1	Proton acceptor By similarity HAMAP MF_01684
	Binding site	82	1	Substrate; via amide nitrogen By similarity HAMAP MF_01684
	Binding site	156	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity HAMAP MF_01684
	Binding site	201	1	Substrate By similarity HAMAP MF_01684

Sequences

	Sequence		Length	Mass (Da)
	B9E6Y7 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 28006199F3522476	FASTA	233	25,091
	10 20 30 40 50 60 MWRKIMIGII GAMEEEVAIL KSEIQNLTTE KIAHVEIYRG ILYGKDVVLM QSGIGKVNAS 70 80 90 100 110 120 ICTTLLIANF KPDYIINTGS AGGLGSGLKV GDILVSTDVL HHDVDATAFG YAQGQVPMMP 130 140 150 160 170 180 ETYKADDMLI DKTKGAISKH NYTAQTGLIV SGDSFIGSAE MKASILEKFP HAMAVEMEAA 190 200 210 220 230 AVAQTCYQFN TPFIITRAVS DLANGEAEMS FEQFLKVACV SSSNIVKSLL ETL

References

[1]

"Complete genome sequence of Macrococcus caseolyticus strain JCSCS5402, reflecting the ancestral genome of the human-pathogenic staphylococci." Baba T., Kuwahara-Arai K., Uchiyama I., Takeuchi F., Ito T., Hiramatsu K. J. Bacteriol. 191:1180-1190(2009) [PubMed: 19074389] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP009484 Genomic DNA. Translation: BAH17955.1.
RefSeq	YP_002560651.1. NC_011999.1.
3D structure databases
ProteinModelPortal	B9E6Y7.
ModBase	Search...
Protein-protein interaction databases
STRING	B9E6Y7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7389191.
GenomeReviews	Gene locus MCCL_1248 in contig AP009484_GR.
KEGG	mcl:MCCL_1248.
PATRIC	22424879. VBIMacCas48391_1381.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LEHFPTM.
ProtClustDB	CLSK885455.
Family and domain databases
HAMAP	MF_01684. Salvage_tnN. [Tree]
InterPro	IPR010049. MTA_SAH_Nsdase. IPR018017. Nucleoside_phosphorylase. IPR000845. Nucleoside_phosphorylase_d. [Graphical view]
KO	K01243.
PANTHER	PTHR21234. PNP_UDP. 1 hit. PTHR21234:SF6. PTHR21234:SF6. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01704. MTA/SAH-Nsdase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B9E6Y7_MACCJ

Accession

Primary (citable) accession number: B9E6Y7

Entry history

Integrated into UniProtKB/TrEMBL:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	December 14, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)