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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Clostridium kluyveri (strain NBRC 12016)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi333 – 3331Zinc; catalyticUniRule annotation
Metal bindingi384 – 3841Zinc; catalyticUniRule annotation
Metal bindingi509 – 5091Zinc; catalyticUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. threonine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. threonyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCKLU583346:GJNQ-2883-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:CKR_2834
OrganismiClostridium kluyveri (strain NBRC 12016)
Taxonomic identifieri583346 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000007969 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637Threonine--tRNA ligasePRO_1000199538Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi583346.CKR_2834.

Structurei

3D structure databases

ProteinModelPortaliB9E5W0.
SMRiB9E5W0. Positions 241-633.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni242 – 532291CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000003879.
KOiK01868.
OrthoDBiEOG61KBFJ.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9E5W0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKISLKNGK EIEVEKGLKV IDIAAKLSIS LSKKALGAVV DGKVVELNYK
60 70 80 90 100
INKDCKVEIL TFEDEEGKKI LRHTASHILA QAIKRLYPEV KLAIGPAIDS
110 120 130 140 150
GFYYDVDAEF SFTPELLEKI EGKMNEIIKE NIKLERFELP REEAIKFMEE
160 170 180 190 200
KNEPYKVELI KDLPEDSIIS FYKQGDFVDL CAGPHVPSTG RAKAVKLLSI
210 220 230 240 250
AGAYWRGNEN NKMLQRIYGT VFEKKSDLQD YLKLMEEAKK RDHRKLGKEL
260 270 280 290 300
DLFSIHEEGP GFPFFHPKGM VIRNTLQNFW REMHYKADYS EIMTPIILNE
310 320 330 340 350
ELWHRSGHWD HYKENMYFTK IDDGNYAIKP MNCPGSILVY KNDIRSYRDL
360 370 380 390 400
PKRYAEMGVV HRHEKSGALH GLMRVRCFTQ DDAHIFVTKD DIADEIIKVI
410 420 430 440 450
DLIDNFYKIF GFEYFVELST RPEDSMGSDE DWEAATEGLK NALKMVGLDY
460 470 480 490 500
KINEGDGAFY GPKIDFHLKD CIGRTWQCGT VQLDFQMPEK FDLNYIGADG
510 520 530 540 550
EKHRPVMIHR VVFGSIERFI GILIEHYAGA FPAWIAPVQV QVMNITDAQA
560 570 580 590 600
DYVAEVAKTL KENNIRVECD IRNEKIGYKI REAQMHKVPY MIILGDKEMK
610 620 630
DKNISVRSRK EGDIGAMSLE DFILKLKEEI DKKISHV
Length:637
Mass (Da):73,463
Last modified:March 23, 2009 - v1
Checksum:i4BD227D00D952C0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009049 Genomic DNA. Translation: BAH07885.1.
RefSeqiWP_012103541.1. NC_011837.1.
YP_002473299.1. NC_011837.1.

Genome annotation databases

EnsemblBacteriaiBAH07885; BAH07885; CKR_2834.
KEGGickr:CKR_2834.
PATRICi19475913. VBICloKlu118830_3199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009049 Genomic DNA. Translation: BAH07885.1.
RefSeqiWP_012103541.1. NC_011837.1.
YP_002473299.1. NC_011837.1.

3D structure databases

ProteinModelPortaliB9E5W0.
SMRiB9E5W0. Positions 241-633.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi583346.CKR_2834.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH07885; BAH07885; CKR_2834.
KEGGickr:CKR_2834.
PATRICi19475913. VBICloKlu118830_3199.

Phylogenomic databases

eggNOGiCOG0441.
HOGENOMiHOG000003879.
KOiK01868.
OrthoDBiEOG61KBFJ.

Enzyme and pathway databases

BioCyciCKLU583346:GJNQ-2883-MONOMER.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Clostridium kluyveri and comparative genomics of Clostridia species."
    Inui M., Nonaka H., Shinoda Y., Ikenaga Y., Abe M., Naito K., Vertes A.A., Yukawa H.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NBRC 12016.

Entry informationi

Entry nameiSYT_CLOK1
AccessioniPrimary (citable) accession number: B9E5W0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2009
Last sequence update: March 23, 2009
Last modified: March 31, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.