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B9E4X8

- TRMB_CLOK1

UniProt

B9E4X8 - TRMB_CLOK1

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Clostridium kluyveri (strain NBRC 12016)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei43 – 431S-adenosyl-L-methionineUniRule annotation
    Binding sitei68 – 681S-adenosyl-L-methionineUniRule annotation
    Binding sitei95 – 951S-adenosyl-L-methionineUniRule annotation
    Binding sitei117 – 1171S-adenosyl-L-methionineUniRule annotation
    Binding sitei121 – 1211SubstrateUniRule annotation
    Binding sitei153 – 1531SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCKLU583346:GJNQ-2547-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:CKR_2502
    OrganismiClostridium kluyveri (strain NBRC 12016)
    Taxonomic identifieri583346 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000007969: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 211211tRNA (guanine-N(7)-)-methyltransferasePRO_1000149653Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi583346.CKR_2502.

    Structurei

    3D structure databases

    ProteinModelPortaliB9E4X8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni190 – 1934Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000251689.
    KOiK03439.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9E4X8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLRKKWWAR PEMEASNLTI INPSLFKGKW KDEFKNDGEI YLELGCGRGK    50
    FLCTQALNNK HINYIGIDLK DEVLIYALKK VVEAEAENIR IIPMNISKIE 100
    NVFDRDEVGR IYINFCNPWP KRRQKKRRLT HTGFLNIYKK FLKPKSEVWF 150
    KTDDRGLFEE SQEYFVESGF SIDYITYDLH DSGFTDNVVT EYEEKFTNLG 200
    MKTMFLIARI V 211
    Length:211
    Mass (Da):25,059
    Last modified:March 24, 2009 - v1
    Checksum:i5EE336D2CA7ED602
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009049 Genomic DNA. Translation: BAH07553.1.
    RefSeqiWP_012103152.1. NC_011837.1.
    YP_002472967.1. NC_011837.1.

    Genome annotation databases

    EnsemblBacteriaiBAH07553; BAH07553; CKR_2502.
    GeneIDi7273659.
    KEGGickr:CKR_2502.
    PATRICi19475130. VBICloKlu118830_2812.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP009049 Genomic DNA. Translation: BAH07553.1 .
    RefSeqi WP_012103152.1. NC_011837.1.
    YP_002472967.1. NC_011837.1.

    3D structure databases

    ProteinModelPortali B9E4X8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 583346.CKR_2502.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAH07553 ; BAH07553 ; CKR_2502 .
    GeneIDi 7273659.
    KEGGi ckr:CKR_2502.
    PATRICi 19475130. VBICloKlu118830_2812.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000251689.
    KOi K03439.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci CKLU583346:GJNQ-2547-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Clostridium kluyveri and comparative genomics of Clostridia species."
      Inui M., Nonaka H., Shinoda Y., Ikenaga Y., Abe M., Naito K., Vertes A.A., Yukawa H.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NBRC 12016.

    Entry informationi

    Entry nameiTRMB_CLOK1
    AccessioniPrimary (citable) accession number: B9E4X8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3