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B9E1G8 (RNC_CLOK1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Ribonuclease III
Short name=RNase III
Gene names
Name:rnc
Ordered Locus Names:CKR_1292
OrganismClostridium kluyveri (strain NBRC 12016) [Complete proteome] [HAMAP]
Taxonomic identifier583346 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length244 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104

Cofactor

Mg2+ By similarity. HAMAP-Rule MF_00104

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00104

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00104.

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 RNase III domain.

Ontologies

Keywords
   Biological processmRNA processing
rRNA processing
tRNA processing
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
RNA-binding
rRNA-binding
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

rRNA catabolic process

Inferred from electronic annotation. Source: InterPro

rRNA processing

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

rRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribonuclease III activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 244244Ribonuclease 3 HAMAP-Rule MF_00104
PRO_1000194420

Regions

Domain7 – 134128RNase III
Domain161 – 23070DRBM

Sites

Active site511 Potential
Active site1231 By similarity
Metal binding471Magnesium By similarity
Metal binding1201Magnesium By similarity
Metal binding1231Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
B9E1G8 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 5A0F5AB4F6CCA482

FASTA24428,029
        10         20         30         40         50         60 
MEKVNFFEEV EKTLNISFND KELIDTALTH SSYANGKKGV KFNERMEFLG DSVLQLCISE 

        70         80         90        100        110        120 
YLFLIYKSKS EGELTKKRSL IVCENSLYEV AKKWNIGKYI KMSKGEEITG GRERTSILAN 

       130        140        150        160        170        180 
CVEAIIAAIY IDSGYKKTKQ FIIDNFKDII EKAIKNQIVL DYKTNLQEIV QQDGDIHIEY 

       190        200        210        220        230        240 
MLIKYEGPPH RRKFYTKVCV ANNVMGSGVG YTKKESEQNA AQDALKKLKS EDKWNKEGID 


TNEK 

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References

[1]"Complete genome sequence of Clostridium kluyveri and comparative genomics of Clostridia species."
Inui M., Nonaka H., Shinoda Y., Ikenaga Y., Abe M., Naito K., Vertes A.A., Yukawa H.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBRC 12016.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009049 Genomic DNA. Translation: BAH06343.1.
RefSeqYP_002471757.1. NC_011837.1.

3D structure databases

ProteinModelPortalB9E1G8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING583346.CKR_1292.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAH06343; BAH06343; CKR_1292.
GeneID7274307.
KEGGckr:CKR_1292.
PATRIC19472420. VBICloKlu118830_1457.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0571.
HOGENOMHOG000246808.
KOK03685.
OrthoDBEOG6T1WVS.
ProtClustDBPRK00102.

Enzyme and pathway databases

BioCycCKLU583346:GJNQ-1337-MONOMER.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPMF_00104. RNase_III.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
TIGRFAMsTIGR02191. RNaseIII. 1 hit.
PROSITEPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNC_CLOK1
AccessionPrimary (citable) accession number: B9E1G8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: April 16, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families