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B9E0B1 (ASSY_CLOK1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:CKR_0885
OrganismClostridium kluyveri (strain NBRC 12016) [Complete proteome] [HAMAP]
Taxonomic identifier583346 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 401401Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_1000191890

Regions

Nucleotide binding8 – 169ATP By similarity

Sites

Binding site861Citrulline By similarity
Binding site911Citrulline By similarity
Binding site1161ATP; via amide nitrogen By similarity
Binding site1181Aspartate By similarity
Binding site1221Aspartate By similarity
Binding site1221Citrulline By similarity
Binding site1231Aspartate By similarity
Binding site1261Citrulline By similarity
Binding site1751Citrulline By similarity
Binding site1841Citrulline By similarity
Binding site2601Citrulline By similarity
Binding site2721Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
B9E0B1 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 9F3032FA39B64220

FASTA40144,802
        10         20         30         40         50         60 
MKDKVVLAYS GGLDTSIIIP WLKENYDLDV IAACIDVGQD DDMEAVRNKA IKTGAVKVYI 

        70         80         90        100        110        120 
EDVKEEFVRD YLFSAVKAHI LYEDAYLLGT SLARPLMAKR LVEIAHAEGA KYIAHGCTGK 

       130        140        150        160        170        180 
GNDQVRFEVG VASFDPKLGI IAPWRIWDIK SREDAIDYAN SKGVEVPVTK EKIYSNDKNI 

       190        200        210        220        230        240 
WHVSHEGGDL EDPKNEHKSS MYFMTTPPEK AKDEVSYVEL YFEQGIPKKL DGKELPPVEM 

       250        260        270        280        290        300 
MQTLNKLGGE NGIGIVDMVE NRLVGMKSRG VYETPGGTIL YAAHAALERL TIDKNTAHYK 

       310        320        330        340        350        360 
QMISQKYGEL VYDGLWFSPL KEALDAFVEV TQKNVTGSVK LKLYKGNVMV AGVDAPYALY 

       370        380        390        400 
DEDISSFGAS ELYDHKDAEG FIKIFSLPYK IKAMIEKEKG N 

« Hide

References

[1]"Complete genome sequence of Clostridium kluyveri and comparative genomics of Clostridia species."
Inui M., Nonaka H., Shinoda Y., Ikenaga Y., Abe M., Naito K., Vertes A.A., Yukawa H.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBRC 12016.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009049 Genomic DNA. Translation: BAH05936.1.
RefSeqYP_002471350.1. NC_011837.1.

3D structure databases

ProteinModelPortalB9E0B1.
SMRB9E0B1. Positions 4-397.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING583346.CKR_0885.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAH05936; BAH05936; CKR_0885.
GeneID7275725.
KEGGckr:CKR_0885.
PATRIC19471578. VBICloKlu118830_1036.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OrthoDBEOG6K9QCV.
ProtClustDBPRK00509.

Enzyme and pathway databases

BioCycCKLU583346:GJNQ-930-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_CLOK1
AccessionPrimary (citable) accession number: B9E0B1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: February 19, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways