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B9DYW1 (FTHS_CLOK1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:CKR_0385
OrganismClostridium kluyveri (strain NBRC 12016) [Complete proteome] [HAMAP]
Taxonomic identifier583346 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length558 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 558558Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_1000185253

Regions

Nucleotide binding66 – 738ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B9DYW1 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 6E7ABE3A88C40AE8

FASTA55860,414
        10         20         30         40         50         60 
MNFKSDIQIA QECSMENIKD IAKKLNIFED EIELYGKYKA KIDYNLLKTT KGKNGKLILC 

        70         80         90        100        110        120 
TAINPTPAGE GKTTTSIGVA DALARLDKSV VVALREPSMG PVFGIKGGAA GGGYAQVVPM 

       130        140        150        160        170        180 
EDINLHFTGD IHAMTAANNL LAALIDNHIY QGNKLNIDSR RVVWRRCVDM NDRQLRFVVD 

       190        200        210        220        230        240 
GLGGKVNGIP REDGFDITVA SEIMAIFCLS TDINDLKERI SKIVVGYTTE GNPVTAHDLK 

       250        260        270        280        290        300 
AEGAMAALLK DALKPNLVQT LEGTPAFVHG GPFANIAHGC NSIMATRMAM HFGDYVVTEA 

       310        320        330        340        350        360 
GFGADLGAEK FLDIKCRMAG LRPDAVIIVA TVRALKYNGG TPKTKLNNEN LETLEKGIPN 

       370        380        390        400        410        420 
LLKHVENITK VFKLPAVVAL NAFPTDTEAE LKLVEEKCRE FGVSVKLSEV WAKGGEGGIE 

       430        440        450        460        470        480 
VAKEVLRLIN EGKNDFQFAY DEKLPIRDKI RAIAQKIYGA DGVTFTNQAE KEIDELEKLG 

       490        500        510        520        530        540 
FGKTPVCIAK TQYSLTDDQN KLGRPKGFKI TVRQVSISAG AGFVVAITGS IMKMPGLPKV 

       550 
PAAEKIDVDE NGVISGLF 

« Hide

References

[1]"Complete genome sequence of Clostridium kluyveri and comparative genomics of Clostridia species."
Inui M., Nonaka H., Shinoda Y., Ikenaga Y., Abe M., Naito K., Vertes A.A., Yukawa H.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NBRC 12016.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP009049 Genomic DNA. Translation: BAH05436.1.
RefSeqYP_002470850.1. NC_011837.1.

3D structure databases

ProteinModelPortalB9DYW1.
SMRB9DYW1. Positions 6-556.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING583346.CKR_0385.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAH05436; BAH05436; CKR_0385.
GeneID7276342.
KEGGckr:CKR_0385.
PATRIC19470458. VBICloKlu118830_0488.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OrthoDBEOG6PCPSP.
ProtClustDBPRK13505.

Enzyme and pathway databases

BioCycCKLU583346:GJNQ-418-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_CLOK1
AccessionPrimary (citable) accession number: B9DYW1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: April 16, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways