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B9DUU7

- B9DUU7_STRU0

UniProt

B9DUU7 - B9DUU7_STRU0

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptococcus uberis (strain ATCC BAA-854 / 0140J)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
    Binding sitei598 – 5981ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSUBE218495:GJ7D-1318-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Gene namesi
    Name:ileSUniRule annotationImported
    Ordered Locus Names:SUB1284Imported
    OrganismiStreptococcus uberis (strain ATCC BAA-854 / 0140J)Imported
    Taxonomic identifieri218495 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000449: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    PTM / Processingi

    Proteomic databases

    PRIDEiB9DUU7.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi218495.SUB1284.

    Structurei

    3D structure databases

    ProteinModelPortaliB9DUU7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionUniRule annotationAdd
    BLAST
    Motifi595 – 5995"KMSKS" regionUniRule annotation

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9DUU7-1 [UniParc]FASTAAdd to Basket

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    MKLKETLNLG KTAFPMRAGL PNKEPLWQAA WDEAQIYQKR QKLNEGKPSF    50
    HLHDGPPYAN GNIHVGHALN KISKDIIVRA KSMSGYNAPY VPGWDTHGLP 100
    IEQVLAKKGI KRKEMDLAEY LEMCRDYALS QVDKQRQDFK RLGVSADWDN 150
    PYVTLDPAYE ADQVRVFGAM ADKGYIYRGA KPVYWSWSSE SALAEAEIEY 200
    HDIDSTSLYY ANKVKDGKGI LDTDSYIVVW TTTPFTVTAS RGLTVGPDMD 250
    YVLVQPASTS KKYLVAEGLL DSLAPKFAWT DFEILSHHKG SELEYIVTEH 300
    PWDSDVDELV ILGDHVTLDS GTGIVHTAPG FGEDDYNVGT KYKLEVAVTV 350
    DERGMMMENA GPDFQGQFYD KVLPTVIEKL GDLLLAKEVI NHSYPFDWRT 400
    KKPIIWRAVP QWFASVSDFR QEILDEIEKT TFHPSWGKTR LYNMIRDRGD 450
    WVISRQRAWG VPLPIFYAED GTAIMTKEVT DHVANLFEKE GSIIWWKKEA 500
    KELLPEDFSH PGSPNGEFTK ETDIMDVWFD SGSSWNGVMN AREQLSYPAD 550
    LYLEGSDQYR GWFNSSLITS VAVNGHAPYK AVLSQGFVLD GKGEKMSKSK 600
    GNIISPNDVA KQYGAEILRL WVTSVDTDND VRVSMDILGQ VSETYRKIRN 650
    TLRFLIANTS DFNPNTNRVA YEELAPVDKY LTIKFNKLVE VFNNSYENYD 700
    FMAIYKAVVN FVTIDLSAFY LDFAKDVVYI EAANSLARRR MQTVFYDILV 750
    KITKLLTPIL PHTAEEIWSY LEFEEEEFVQ LTEMPVAEIF EGEEDILSAW 800
    DAFMTLRTQA QKALEEARNE KVIGKSLEAH LTIYASDEVK TLLTALDSDI 850
    ALLMIVSQLT IADFADAPTN AISFENVAFT VDHAVGEVCE RSRRVDPTTR 900
    MRSYGAFVCD ASAKIIEENF PEAVSQGFES N 931
    Length:931
    Mass (Da):105,427
    Last modified:March 24, 2009 - v1
    Checksum:i89969FB79AAD126D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM946015 Genomic DNA. Translation: CAR42801.1.
    RefSeqiYP_002562588.1. NC_012004.1.

    Genome annotation databases

    EnsemblBacteriaiCAR42801; CAR42801; SUB1284.
    GeneIDi7391876.
    KEGGisub:SUB1284.
    PATRICi19808276. VBIStrUbe20775_1254.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM946015 Genomic DNA. Translation: CAR42801.1 .
    RefSeqi YP_002562588.1. NC_012004.1.

    3D structure databases

    ProteinModelPortali B9DUU7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 218495.SUB1284.

    Proteomic databases

    PRIDEi B9DUU7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAR42801 ; CAR42801 ; SUB1284 .
    GeneIDi 7391876.
    KEGGi sub:SUB1284.
    PATRICi 19808276. VBIStrUbe20775_1254.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SUBE218495:GJ7D-1318-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-854 / 0140JImported.

    Entry informationi

    Entry nameiB9DUU7_STRU0
    AccessioniPrimary (citable) accession number: B9DUU7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 24, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3