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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptococcus uberis (strain ATCC BAA-854 / 0140J)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciSUBE218495:GJ7D-1318-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotationSAAS annotation (EC:6.1.1.5UniRule annotationSAAS annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:ileSUniRule annotationImported
Ordered Locus Names:SUB1284Imported
OrganismiStreptococcus uberis (strain ATCC BAA-854 / 0140J)Imported
Taxonomic identifieri218495 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000449 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiB9DUU7.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi218495.SUB1284.

Structurei

3D structure databases

ProteinModelPortaliB9DUU7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi595 – 5995"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9DUU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKETLNLG KTAFPMRAGL PNKEPLWQAA WDEAQIYQKR QKLNEGKPSF
60 70 80 90 100
HLHDGPPYAN GNIHVGHALN KISKDIIVRA KSMSGYNAPY VPGWDTHGLP
110 120 130 140 150
IEQVLAKKGI KRKEMDLAEY LEMCRDYALS QVDKQRQDFK RLGVSADWDN
160 170 180 190 200
PYVTLDPAYE ADQVRVFGAM ADKGYIYRGA KPVYWSWSSE SALAEAEIEY
210 220 230 240 250
HDIDSTSLYY ANKVKDGKGI LDTDSYIVVW TTTPFTVTAS RGLTVGPDMD
260 270 280 290 300
YVLVQPASTS KKYLVAEGLL DSLAPKFAWT DFEILSHHKG SELEYIVTEH
310 320 330 340 350
PWDSDVDELV ILGDHVTLDS GTGIVHTAPG FGEDDYNVGT KYKLEVAVTV
360 370 380 390 400
DERGMMMENA GPDFQGQFYD KVLPTVIEKL GDLLLAKEVI NHSYPFDWRT
410 420 430 440 450
KKPIIWRAVP QWFASVSDFR QEILDEIEKT TFHPSWGKTR LYNMIRDRGD
460 470 480 490 500
WVISRQRAWG VPLPIFYAED GTAIMTKEVT DHVANLFEKE GSIIWWKKEA
510 520 530 540 550
KELLPEDFSH PGSPNGEFTK ETDIMDVWFD SGSSWNGVMN AREQLSYPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSLITS VAVNGHAPYK AVLSQGFVLD GKGEKMSKSK
610 620 630 640 650
GNIISPNDVA KQYGAEILRL WVTSVDTDND VRVSMDILGQ VSETYRKIRN
660 670 680 690 700
TLRFLIANTS DFNPNTNRVA YEELAPVDKY LTIKFNKLVE VFNNSYENYD
710 720 730 740 750
FMAIYKAVVN FVTIDLSAFY LDFAKDVVYI EAANSLARRR MQTVFYDILV
760 770 780 790 800
KITKLLTPIL PHTAEEIWSY LEFEEEEFVQ LTEMPVAEIF EGEEDILSAW
810 820 830 840 850
DAFMTLRTQA QKALEEARNE KVIGKSLEAH LTIYASDEVK TLLTALDSDI
860 870 880 890 900
ALLMIVSQLT IADFADAPTN AISFENVAFT VDHAVGEVCE RSRRVDPTTR
910 920 930
MRSYGAFVCD ASAKIIEENF PEAVSQGFES N
Length:931
Mass (Da):105,427
Last modified:March 24, 2009 - v1
Checksum:i89969FB79AAD126D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM946015 Genomic DNA. Translation: CAR42801.1.
RefSeqiWP_012658758.1. NC_012004.1.

Genome annotation databases

EnsemblBacteriaiCAR42801; CAR42801; SUB1284.
GeneIDi24162052.
KEGGisub:SUB1284.
PATRICi19808276. VBIStrUbe20775_1254.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM946015 Genomic DNA. Translation: CAR42801.1.
RefSeqiWP_012658758.1. NC_012004.1.

3D structure databases

ProteinModelPortaliB9DUU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi218495.SUB1284.

Proteomic databases

PRIDEiB9DUU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR42801; CAR42801; SUB1284.
GeneIDi24162052.
KEGGisub:SUB1284.
PATRICi19808276. VBIStrUbe20775_1254.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciSUBE218495:GJ7D-1318-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-854 / 0140JImported.

Entry informationi

Entry nameiB9DUU7_STRU0
AccessioniPrimary (citable) accession number: B9DUU7
Entry historyi
Integrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: July 22, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.