B9DS79 (DAPA_STRU0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	SUB0938

Organism

Streptococcus uberis (strain ATCC BAA-854 / 0140J) [Complete proteome] [HAMAP]

Taxonomic identifier

218495 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000134880
Regions
	Region	54 – 55	2	Pyruvate binding By similarity
Sites
	Active site	168	1	Schiff-base intermediate with substrate By similarity
	Binding site	113	1	Pyruvate By similarity
	Site	140	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9DS79 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 572B16F3DB9E1AC9	FASTA	309	33,808
	10 20 30 40 50 60 MTLSQLKNCQ IITALVTPFH ADGSINFKAL PELIEHLLNN HTQALLLAGT TAESPTLTHE 70 80 90 100 110 120 EELELFSEVQ RIVNGRVPLI AGIGTNDTRD SVDFAKEVAN FGGFAAGLAI VPYYNKPTQE 130 140 150 160 170 180 GLYQHFLAIA EASSLPIIIY NIPGRVVVDL HPDTLLKLAQ HPNIIGVKDC TNLDHLAYII 190 200 210 220 230 240 ENKPEDFLIY TGEDGQIFHA MNLGADGVIS VASHTNGSEI FEMINHITNG RIKEAAQIQR 250 260 270 280 290 300 RFLPKVNALF SVSSPAPVKA VLNYLGFNVG PLRLPLVACT RQEADAIINI VLDKAPKEYV TKGVLRPDY

References

[1]

"Evidence for niche adaptation in the genome of the bovine pathogen Streptococcus uberis." Ward P.N., Holden M.T.G., Leigh J.A., Lennard N., Bignell A., Barron A., Clark L., Quail M.A., Woodward J., Barrell B.G., Egan S.A., Field T.R., Maskell D., Kehoe M., Dowson C.G., Chanter N., Whatmore A.M., Bentley S.D., Parkhill J. BMC Genomics 10:54-54(2009) [PubMed: 19175920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-854 / 0140J.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM946015 Genomic DNA. Translation: CAR42088.1.
RefSeq	YP_002562264.1. NC_012004.1.
3D structure databases
ProteinModelPortal	B9DS79.
ModBase	Search...
Protein-protein interaction databases
STRING	B9DS79.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000069826; EBSTRP00000065861; EBSTRG00000068837.
GeneID	7391408.
GenomeReviews	Gene locus SUB0938 in contig AM946015_GR.
KEGG	sub:SUB0938.
PATRIC	19807572. VBIStrUbe20775_0906.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000028194.
OMA	CEMEDSN.
ProtClustDB	PRK03170.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_STRU0

Accession

Primary (citable) accession number: B9DS79

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program