B9DS62 (TRMFO_STRU0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
EC=2.1.1.74

Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Ordered Locus Names:	SUB0921

Organism

Streptococcus uberis (strain ATCC BAA-854 / 0140J) [Complete proteome] [HAMAP]

Taxonomic identifier

218495 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus uberis

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037
Catalytic activity	5,10-methylenetetrahydrofolate + uridine54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluridine54 in tRNA + FAD. HAMAP-Rule MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Inferred from electronic annotation. Source: HAMAP flavin adenine dinucleotide binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037		PRO_1000149479
Regions
	Nucleotide binding	10 – 15	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9DS62 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: B66A7FE7F52C9647	FASTA	444	49,211
	10 20 30 40 50 60 MSQSYINVIG AGLAGSEAAY QIAKRGIPVK LYEMRGVKAT PQHKTSNFAE LVCSNSFRGD 70 80 90 100 110 120 SLTNAVGLLK EEMRRLDSII MRAGEAHRVP AGGAMAVDRV GYAEAVTAEL ENNPLIEVIR 130 140 150 160 170 180 GEITEIPNDA ITVIATGPLT SDALAEKIHA LNGGEGFYFY DAAAPIIDKS TINMDKVYLK 190 200 210 220 230 240 SRYDKGEAAY LNCPMTKEEF LAFHEALTTA EEAPLNSFEK EKYFEGCMPI EVMAKRGIKT 250 260 270 280 290 300 MLYGPMKPVG LEYPDDYKGP RDGDYKTPYA VVQLRQDNAA GSLYNMVGFQ THLKWGEQKR 310 320 330 340 350 360 VFQMIPGLEN AEFVRYGVMH RNSYMDSPNL LKQTFQSRAN ENLFFAGQMT GVEGYVESAA 370 380 390 400 410 420 SGLVAGINAA RLFKGEEAII FPETTAIGSL PHYVTHTDSK HFQPMNVNFG IIKELEGKRI 430 440 RDKKERYEAI AERSLKDLEA FLNS

References

[1]

"Evidence for niche adaptation in the genome of the bovine pathogen Streptococcus uberis." Ward P.N., Holden M.T.G., Leigh J.A., Lennard N., Bignell A., Barron A., Clark L., Quail M.A., Woodward J., Barrell B.G., Egan S.A., Field T.R., Maskell D., Kehoe M., Dowson C.G., Chanter N., Whatmore A.M., Bentley S.D., Parkhill J. BMC Genomics 10:54-54(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-854 / 0140J.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM946015 Genomic DNA. Translation: CAR42047.1.
RefSeq	YP_002562247.1. NC_012004.1.
3D structure databases
ProteinModelPortal	B9DS62.
ModBase	Search...
Protein-protein interaction databases
STRING	218495.SUB0921.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR42047; CAR42047; SUB0921.
GeneID	7391394.
KEGG	sub:SUB0921.
PATRIC	19807538. VBIStrUbe20775_0889.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1206.
HOGENOM	HOG000252054.
KO	K04094.
OMA	RFAGQIT.
ProtClustDB	PRK05335.
Family and domain databases
HAMAP	MF_01037. TrmFO.
InterPro	IPR004417. Folate-dep_Ribothymidyl_synth. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_STRU0

Accession

Primary (citable) accession number: B9DS62

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program