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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Streptococcus uberis (strain ATCC BAA-854 / 0140J)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotationImported
Ordered Locus Names:SUB0459Imported
OrganismiStreptococcus uberis (strain ATCC BAA-854 / 0140J)Imported
Taxonomic identifieri218495 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000449 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi218495.SUBp02.

Structurei

3D structure databases

ProteinModelPortaliB9DRC8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini565 – 668TRNA-bindingInterPro annotationAdd BLAST104

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi14 – 24"HIGH" regionUniRule annotationAdd BLAST11
Motifi311 – 315"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
OMAiTYVWFDA.
OrthoDBiPOG091H0269.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
cd02800. tRNA_bind_EcMetRS_like. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiView protein in InterPro
IPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
PfamiView protein in Pfam
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiView protein in PROSITE
PS50886. TRBD. 1 hit.

Sequencei

Sequence statusi: Complete.

B9DRC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKKPFYIT TPIYYPSGKL HIGSAYTTIA CDVLARYKRM MNHQVFYLTG
60 70 80 90 100
LDEHGQKIQT KAEEAGITPQ EYVDGMAKGV KELWELLDIS YDKFIRTTDD
110 120 130 140 150
YHEEVVAAVF EKLLAQDDIY LGEYSGWYSV SDEEFFTESQ LEEVFRDENG
160 170 180 190 200
NVIGGIAPSG HEVEWVSEES YFLRLGKYAD KLVAFFNEHP EFIQPEGRMN
210 220 230 240 250
EMIKNFIEPG LEDLAVSRTT FTWGVKVPSN PKHVVYVWID ALLNYATALG
260 270 280 290 300
YGQEDKANFE TFWNNGTVFH MVGKDILRFH SIYWPILLMM LDMKLPERLI
310 320 330 340 350
AHGWFVMKDG KMSKSKGNVV YPEMLVERFG LDPLRYYLMR SLPVGSDGTF
360 370 380 390 400
TPEDYVGRIN YELANDLGNL LNRTVAMINK YFGGKIPAYT ENVTAFDGEL
410 420 430 440 450
QDLVTEKITD YHKQMETVDY PRALDAIWAI ISRANKYIDE TAPWVLAKED
460 470 480 490 500
GDKEQLASVM AHLAASLRVV AHLIQPFMMT TSNAIMEQLG LGQSFDLENL
510 520 530 540 550
SLAGMPSDIT VISKGQPIFP RLDMEEEIAY IKEQMEGGSA IPQEKEWIPE
560 570 580 590 600
EVDLKSEKEE IKFETFDACE IRVAEVKEVS KVEGSDKLLR FRLDAGDGED
610 620 630 640 650
RQILSGIAKF YPNEQELVGK KLQIVANLKP RKMMKKYVSQ GMILSAEHDG
660
KLTVLTVDPA VPNGSIIG
Length:668
Mass (Da):75,922
Last modified:March 24, 2009 - v1
Checksum:i93731954856237CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM946015 Genomic DNA. Translation: CAR41140.1.
RefSeqiWP_012657994.1. NC_012004.1.

Genome annotation databases

EnsemblBacteriaiCAR41140; CAR41140; SUB0459.
GeneIDi24162919.
KEGGisub:SUB0459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM946015 Genomic DNA. Translation: CAR41140.1.
RefSeqiWP_012657994.1. NC_012004.1.

3D structure databases

ProteinModelPortaliB9DRC8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi218495.SUBp02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR41140; CAR41140; SUB0459.
GeneIDi24162919.
KEGGisub:SUB0459.

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
OMAiTYVWFDA.
OrthoDBiPOG091H0269.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
cd02800. tRNA_bind_EcMetRS_like. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiView protein in InterPro
IPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
PfamiView protein in Pfam
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiView protein in PROSITE
PS50886. TRBD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB9DRC8_STRU0
AccessioniPrimary (citable) accession number: B9DRC8
Entry historyiIntegrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: June 7, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.