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B9DRC8 (B9DRC8_STRU0) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_01228

EC=6.1.1.10 HAMAP-Rule MF_01228
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_01228
Gene names
Name:metG HAMAP-Rule MF_01228 EMBL CAR41140.1
Ordered Locus Names:SUB0459 EMBL CAR41140.1
OrganismStreptococcus uberis (strain ATCC BAA-854 / 0140J) [Complete proteome] [HAMAP] EMBL CAR41140.1
Taxonomic identifier218495 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length668 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228 SAAS SAAS023457

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228 SAAS SAAS023457

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228 SAAS SAAS023457.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. HAMAP-Rule MF_01228

Contains 1 tRNA-binding domain. HAMAP-Rule MF_01228

Contains tRNA-binding domain. SAAS SAAS014758

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain565 – 668104tRNA-binding By similarity HAMAP-Rule MF_01228
Motif14 – 2411"HIGH" region By similarity HAMAP-Rule MF_01228
Motif311 – 3155"KMSKS" region By similarity HAMAP-Rule MF_01228

Sites

Metal binding1611Zinc By similarity HAMAP-Rule MF_01228

Sequences

Sequence LengthMass (Da)Tools
B9DRC8 [UniParc].

Last modified March 24, 2009. Version 1.
Checksum: 93731954856237CE

FASTA66875,922
        10         20         30         40         50         60 
MTDKKPFYIT TPIYYPSGKL HIGSAYTTIA CDVLARYKRM MNHQVFYLTG LDEHGQKIQT 

        70         80         90        100        110        120 
KAEEAGITPQ EYVDGMAKGV KELWELLDIS YDKFIRTTDD YHEEVVAAVF EKLLAQDDIY 

       130        140        150        160        170        180 
LGEYSGWYSV SDEEFFTESQ LEEVFRDENG NVIGGIAPSG HEVEWVSEES YFLRLGKYAD 

       190        200        210        220        230        240 
KLVAFFNEHP EFIQPEGRMN EMIKNFIEPG LEDLAVSRTT FTWGVKVPSN PKHVVYVWID 

       250        260        270        280        290        300 
ALLNYATALG YGQEDKANFE TFWNNGTVFH MVGKDILRFH SIYWPILLMM LDMKLPERLI 

       310        320        330        340        350        360 
AHGWFVMKDG KMSKSKGNVV YPEMLVERFG LDPLRYYLMR SLPVGSDGTF TPEDYVGRIN 

       370        380        390        400        410        420 
YELANDLGNL LNRTVAMINK YFGGKIPAYT ENVTAFDGEL QDLVTEKITD YHKQMETVDY 

       430        440        450        460        470        480 
PRALDAIWAI ISRANKYIDE TAPWVLAKED GDKEQLASVM AHLAASLRVV AHLIQPFMMT 

       490        500        510        520        530        540 
TSNAIMEQLG LGQSFDLENL SLAGMPSDIT VISKGQPIFP RLDMEEEIAY IKEQMEGGSA 

       550        560        570        580        590        600 
IPQEKEWIPE EVDLKSEKEE IKFETFDACE IRVAEVKEVS KVEGSDKLLR FRLDAGDGED 

       610        620        630        640        650        660 
RQILSGIAKF YPNEQELVGK KLQIVANLKP RKMMKKYVSQ GMILSAEHDG KLTVLTVDPA 


VPNGSIIG 

« Hide

References

[1]"Evidence for niche adaptation in the genome of the bovine pathogen Streptococcus uberis."
Ward P.N., Holden M.T.G., Leigh J.A., Lennard N., Bignell A., Barron A., Clark L., Quail M.A., Woodward J., Barrell B.G., Egan S.A., Field T.R., Maskell D., Kehoe M., Dowson C.G., Chanter N., Whatmore A.M., Bentley S.D., Parkhill J.
BMC Genomics 10:54-54(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-854 / 0140J.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM946015 Genomic DNA. Translation: CAR41140.1.
RefSeqYP_002561812.1. NC_012004.1.

3D structure databases

ProteinModelPortalB9DRC8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING218495.SUBp02.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAR41140; CAR41140; SUB0459.
GeneID7392232.
KEGGsub:SUBp02.
PATRIC19806638. VBIStrUbe20775_0440.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
KOK01874.
OMAANEWGNL.
OrthoDBEOG6CVV9B.
ProtClustDBPRK12267.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamPF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
PROSITEPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB9DRC8_STRU0
AccessionPrimary (citable) accession number: B9DRC8
Entry history
Integrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: April 16, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)