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B9DPP7

- SYI_STACT

UniProt

B9DPP7 - SYI_STACT

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus carnosus (strain TM300)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation
Metal bindingi885 – 8851ZincUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSCAR396513:GJ9G-821-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Sca_0806
OrganismiStaphylococcus carnosus (strain TM300)
Taxonomic identifieri396513 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000444: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 915915Isoleucine--tRNA ligasePRO_1000189198Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi396513.Sca_0806.

Structurei

3D structure databases

ProteinModelPortaliB9DPP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9DPP7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNYKDTLLMP KTDFPMRGGL PKKEPEIQKQ WDEQDLYHKI LEKNKGHESY
60 70 80 90 100
ILHDGPPYAN GNLHMGHALN KILKDFIVRY KSMQGYYSPY VPGWDTHGLP
110 120 130 140 150
IEQALTKKGV KRKEMPISEF RKLCEEFALE QIDIQKKDFK RLGVNGDFND
160 170 180 190 200
PYITLKPEYE AAQIRLFGEM ADRGLIYKGK KPVYWSPSSE SSLAEAEIEY
210 220 230 240 250
HDKRSPSIYV AFDVKDGKGV VDEDAKFIIW TTTPWTLPSN VAITVHPDLK
260 270 280 290 300
YGQYNVNGEK YVVGQDLVEE VAEELGWDKE DIQLEKEFTG KELEYVETQH
310 320 330 340 350
PFIDRVSLVI NGLHVTTDAG TGAVHTAPGH GEDDYIVGQK YNLPVISPLN
360 370 380 390 400
DKGVFTEEGG QFEGMFYDKA NKAVTDLLKE NGSLLKLKFI THSYPHDWRT
410 420 430 440 450
KKPVIFRATP QWFASISKVR QDILDAIEDT NFKVDWGKTR IYNMIRDRGE
460 470 480 490 500
WVISRQRVWG VPLPVFYTEN GDIIMDKEVI YHVANLFEKY GSNVWFDRDA
510 520 530 540 550
KDLLPEGFTH PGSPNGEFTK EEDIMDVWFD SGSSHRGVLE TRPELSFPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSITTA VATRGQSPYK FLLSHGFVMD GEGKKMSKSL
610 620 630 640 650
GNVIVPDQIV KQKGADIARL WVSSVDYLSD VRISDEILKQ TADVYRKIRN
660 670 680 690 700
TLRFMLGNVN DFNPETDAVP ESDLLEVDRY LLNRLREFTE SIINHYDNFD
710 720 730 740 750
YLNIYQEVQN FINVELSNFY LDYGKDILYI EKQDSHKRRS MQTVLYQILV
760 770 780 790 800
DMTKLLAPIL AHTSEEVWSY IPHVKEESVH LADMPEVVKP DEELLEKWNT
810 820 830 840 850
FMKLRDDVNR ALEEARNNKV IGKSLEAKVI IGSNESFDAA DFLKDFENLH
860 870 880 890 900
QLFIVSQVEV EEKVEDGNEY YYGDIKVVHA DGEKCERCWN YSTELGSVNG
910
LDHLCPRCQG VVADL
Length:915
Mass (Da):105,313
Last modified:March 24, 2009 - v1
Checksum:iCD884BE7F8D48E30
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM295250 Genomic DNA. Translation: CAL27716.1.
RefSeqiWP_015900058.1. NC_012121.1.
YP_002633901.1. NC_012121.1.

Genome annotation databases

GeneIDi7550289.
KEGGisca:Sca_0806.
PATRICi19602386. VBIStaCar105558_0807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM295250 Genomic DNA. Translation: CAL27716.1 .
RefSeqi WP_015900058.1. NC_012121.1.
YP_002633901.1. NC_012121.1.

3D structure databases

ProteinModelPortali B9DPP7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 396513.Sca_0806.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 7550289.
KEGGi sca:Sca_0806.
PATRICi 19602386. VBIStaCar105558_0807.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SCAR396513:GJ9G-821-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300."
    Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C., Goetz F.
    Appl. Environ. Microbiol. 75:811-822(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TM300.

Entry informationi

Entry nameiSYI_STACT
AccessioniPrimary (citable) accession number: B9DPP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3