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B9DPP7

- SYI_STACT

UniProt

B9DPP7 - SYI_STACT

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus carnosus (strain TM300)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
    Binding sitei598 – 5981ATPUniRule annotation
    Metal bindingi885 – 8851ZincUniRule annotation
    Metal bindingi888 – 8881ZincUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSCAR396513:GJ9G-821-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Sca_0806
    OrganismiStaphylococcus carnosus (strain TM300)
    Taxonomic identifieri396513 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000444: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 915915Isoleucine--tRNA ligasePRO_1000189198Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi396513.Sca_0806.

    Structurei

    3D structure databases

    ProteinModelPortaliB9DPP7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi595 – 5995"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGRRSCQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9DPP7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYKDTLLMP KTDFPMRGGL PKKEPEIQKQ WDEQDLYHKI LEKNKGHESY    50
    ILHDGPPYAN GNLHMGHALN KILKDFIVRY KSMQGYYSPY VPGWDTHGLP 100
    IEQALTKKGV KRKEMPISEF RKLCEEFALE QIDIQKKDFK RLGVNGDFND 150
    PYITLKPEYE AAQIRLFGEM ADRGLIYKGK KPVYWSPSSE SSLAEAEIEY 200
    HDKRSPSIYV AFDVKDGKGV VDEDAKFIIW TTTPWTLPSN VAITVHPDLK 250
    YGQYNVNGEK YVVGQDLVEE VAEELGWDKE DIQLEKEFTG KELEYVETQH 300
    PFIDRVSLVI NGLHVTTDAG TGAVHTAPGH GEDDYIVGQK YNLPVISPLN 350
    DKGVFTEEGG QFEGMFYDKA NKAVTDLLKE NGSLLKLKFI THSYPHDWRT 400
    KKPVIFRATP QWFASISKVR QDILDAIEDT NFKVDWGKTR IYNMIRDRGE 450
    WVISRQRVWG VPLPVFYTEN GDIIMDKEVI YHVANLFEKY GSNVWFDRDA 500
    KDLLPEGFTH PGSPNGEFTK EEDIMDVWFD SGSSHRGVLE TRPELSFPAD 550
    LYLEGSDQYR GWFNSSITTA VATRGQSPYK FLLSHGFVMD GEGKKMSKSL 600
    GNVIVPDQIV KQKGADIARL WVSSVDYLSD VRISDEILKQ TADVYRKIRN 650
    TLRFMLGNVN DFNPETDAVP ESDLLEVDRY LLNRLREFTE SIINHYDNFD 700
    YLNIYQEVQN FINVELSNFY LDYGKDILYI EKQDSHKRRS MQTVLYQILV 750
    DMTKLLAPIL AHTSEEVWSY IPHVKEESVH LADMPEVVKP DEELLEKWNT 800
    FMKLRDDVNR ALEEARNNKV IGKSLEAKVI IGSNESFDAA DFLKDFENLH 850
    QLFIVSQVEV EEKVEDGNEY YYGDIKVVHA DGEKCERCWN YSTELGSVNG 900
    LDHLCPRCQG VVADL 915
    Length:915
    Mass (Da):105,313
    Last modified:March 24, 2009 - v1
    Checksum:iCD884BE7F8D48E30
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM295250 Genomic DNA. Translation: CAL27716.1.
    RefSeqiYP_002633901.1. NC_012121.1.

    Genome annotation databases

    GeneIDi7550289.
    KEGGisca:Sca_0806.
    PATRICi19602386. VBIStaCar105558_0807.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM295250 Genomic DNA. Translation: CAL27716.1 .
    RefSeqi YP_002633901.1. NC_012121.1.

    3D structure databases

    ProteinModelPortali B9DPP7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 396513.Sca_0806.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 7550289.
    KEGGi sca:Sca_0806.
    PATRICi 19602386. VBIStaCar105558_0807.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGRRSCQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SCAR396513:GJ9G-821-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300."
      Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C., Goetz F.
      Appl. Environ. Microbiol. 75:811-822(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TM300.

    Entry informationi

    Entry nameiSYI_STACT
    AccessioniPrimary (citable) accession number: B9DPP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3