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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Staphylococcus carnosus (strain TM300)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi490MagnesiumUniRule annotation1
Metal bindingi496MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Sca_0914
OrganismiStaphylococcus carnosus (strain TM300)
Taxonomic identifieri396513 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000444 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001857511 – 701Polyribonucleotide nucleotidyltransferaseAdd BLAST701

Interactioni

Protein-protein interaction databases

STRINGi396513.Sca_0914.

Structurei

3D structure databases

ProteinModelPortaliB9DPE0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini557 – 616KHUniRule annotationAdd BLAST60
Domaini626 – 694S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

B9DPE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQEKKVFKT EWANRPLSIE TGQMAKQANG AVLVRYGDTV VLSTATASKE
60 70 80 90 100
PRDGDFFPLM VNYEEKMYAA GKIPGGFKKR EGRPADEATL TARLIDRPIR
110 120 130 140 150
PLFPKGYRHD VQIINMVLSA DPDCSPEMAA MIGSSMALSV SDIPFQGPIA
160 170 180 190 200
GVNVGYIDGE YVINPTVEQK EVSRLDLEVA GHRDAVNMVE AGASEITEKE
210 220 230 240 250
MLDAIFFGHE EIKRLVDFQQ EIIDYLQPEK TEFIPKERNA EVEEKVTALT
260 270 280 290 300
EEKGLKDAIQ TFDKKEREEN IDAIRDEVVA EFEDEENPDN DEVIAEVNSI
310 320 330 340 350
LNDLVKEEVR RQIADEKVRP DGRKPDEIRP LDSEVGLLPR AHGSGLFTRG
360 370 380 390 400
QTQALSVLTL GAMSEYQLID GLGTEEEKRF MHHYNFPNFS VGETGPVRAP
410 420 430 440 450
GRREIGHGAL GERALRYIIP DTKDFPYTVR IVSEVLESNG SSSQASICGS
460 470 480 490 500
TLALMDAGVP IKAPVAGIAM GLVTRDDNYT ILTDIQGMED ALGDMDFKVA
510 520 530 540 550
GTEKGITAIQ MDIKIDGLTR EIIEEALEQA RVGRLTILNH MLETIDQPRP
560 570 580 590 600
ELSAYAPKVE TMTIKPEKIR DVIGPGGKKI NEIIDETGVK LDIEQDGTIF
610 620 630 640 650
IGAVDQDMIN RAREIIEDIT REAEVGQVYN AKVRRIEKYG TFVELFPGKD
660 670 680 690 700
ALLHISQIAN QRINKVEDVL KLGDTIEVKV TEIDDKGRVN ASHRALLNKE

D
Length:701
Mass (Da):77,859
Last modified:March 24, 2009 - v1
Checksum:i9E58F62952FE275B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM295250 Genomic DNA. Translation: CAL27823.1.
RefSeqiWP_015900164.1. NC_012121.1.

Genome annotation databases

KEGGisca:SCA_0914.
PATRICi19602602. VBIStaCar105558_0915.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM295250 Genomic DNA. Translation: CAL27823.1.
RefSeqiWP_015900164.1. NC_012121.1.

3D structure databases

ProteinModelPortaliB9DPE0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi396513.Sca_0914.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGisca:SCA_0914.
PATRICi19602602. VBIStaCar105558_0915.

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPNP_STACT
AccessioniPrimary (citable) accession number: B9DPE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: February 15, 2017
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.