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B9DN65

- TRMB_STACT

UniProt

B9DN65 - TRMB_STACT

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Staphylococcus carnosus (strain TM300)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431S-adenosyl-L-methionineUniRule annotation
Binding sitei68 – 681S-adenosyl-L-methionineUniRule annotation
Binding sitei95 – 951S-adenosyl-L-methionineUniRule annotation
Active sitei117 – 1171By similarity
Binding sitei117 – 1171S-adenosyl-L-methionineUniRule annotation
Binding sitei121 – 1211SubstrateUniRule annotation
Binding sitei153 – 1531SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSCAR396513:GJ9G-1364-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Sca_1355
OrganismiStaphylococcus carnosus (strain TM300)
Taxonomic identifieri396513 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000444: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211tRNA (guanine-N(7)-)-methyltransferasePRO_1000149663Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi396513.Sca_1355.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni190 – 1934Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiRLVNREF.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9DN65-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLRNKPWAE DYLRKHNTVV DLDGTHAGKM SEWFEKDQPI YIEVGSGMGQ
60 70 80 90 100
FITELAAQHP EVNFVSLERD KNVMIRVLDK VLEKDLQNIK LICNDAMELT
110 120 130 140 150
DYFKDGEVDR VYLNFSDPWP KKRHAKRRLT YHSFLALYKA ILKDEGEIHF
160 170 180 190 200
KTDNRGLFAY SLESMSQFGM YFTKINLNLH DEDDEDNIET EYELKFADKG
210
SRIYRMEAKF H
Length:211
Mass (Da):24,999
Last modified:March 24, 2009 - v1
Checksum:iB50DE0E9C78C8AED
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM295250 Genomic DNA. Translation: CAL28259.1.
RefSeqiWP_015900599.1. NC_012121.1.
YP_002634444.1. NC_012121.1.

Genome annotation databases

GeneIDi7550563.
KEGGisca:Sca_1355.
PATRICi19603474. VBIStaCar105558_1351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM295250 Genomic DNA. Translation: CAL28259.1 .
RefSeqi WP_015900599.1. NC_012121.1.
YP_002634444.1. NC_012121.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 396513.Sca_1355.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 7550563.
KEGGi sca:Sca_1355.
PATRICi 19603474. VBIStaCar105558_1351.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000251689.
KOi K03439.
OMAi RLVNREF.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci SCAR396513:GJ9G-1364-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300."
    Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C., Goetz F.
    Appl. Environ. Microbiol. 75:811-822(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TM300.

Entry informationi

Entry nameiTRMB_STACT
AccessioniPrimary (citable) accession number: B9DN65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: March 24, 2009
Last modified: October 1, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3