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B9DN65

- TRMB_STACT

UniProt

B9DN65 - TRMB_STACT

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Staphylococcus carnosus (strain TM300)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei43 – 431S-adenosyl-L-methionineUniRule annotation
    Binding sitei68 – 681S-adenosyl-L-methionineUniRule annotation
    Binding sitei95 – 951S-adenosyl-L-methionineUniRule annotation
    Active sitei117 – 1171By similarity
    Binding sitei117 – 1171S-adenosyl-L-methionineUniRule annotation
    Binding sitei121 – 1211SubstrateUniRule annotation
    Binding sitei153 – 1531SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSCAR396513:GJ9G-1364-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:Sca_1355
    OrganismiStaphylococcus carnosus (strain TM300)
    Taxonomic identifieri396513 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000444: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 211211tRNA (guanine-N(7)-)-methyltransferasePRO_1000149663Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi396513.Sca_1355.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni190 – 1934Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000251689.
    KOiK03439.
    OMAiRLVNREF.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B9DN65-1 [UniParc]FASTAAdd to Basket

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    MRLRNKPWAE DYLRKHNTVV DLDGTHAGKM SEWFEKDQPI YIEVGSGMGQ    50
    FITELAAQHP EVNFVSLERD KNVMIRVLDK VLEKDLQNIK LICNDAMELT 100
    DYFKDGEVDR VYLNFSDPWP KKRHAKRRLT YHSFLALYKA ILKDEGEIHF 150
    KTDNRGLFAY SLESMSQFGM YFTKINLNLH DEDDEDNIET EYELKFADKG 200
    SRIYRMEAKF H 211
    Length:211
    Mass (Da):24,999
    Last modified:March 24, 2009 - v1
    Checksum:iB50DE0E9C78C8AED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM295250 Genomic DNA. Translation: CAL28259.1.
    RefSeqiWP_015900599.1. NC_012121.1.
    YP_002634444.1. NC_012121.1.

    Genome annotation databases

    GeneIDi7550563.
    KEGGisca:Sca_1355.
    PATRICi19603474. VBIStaCar105558_1351.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM295250 Genomic DNA. Translation: CAL28259.1 .
    RefSeqi WP_015900599.1. NC_012121.1.
    YP_002634444.1. NC_012121.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 396513.Sca_1355.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 7550563.
    KEGGi sca:Sca_1355.
    PATRICi 19603474. VBIStaCar105558_1351.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000251689.
    KOi K03439.
    OMAi RLVNREF.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci SCAR396513:GJ9G-1364-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300."
      Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C., Goetz F.
      Appl. Environ. Microbiol. 75:811-822(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TM300.

    Entry informationi

    Entry nameiTRMB_STACT
    AccessioniPrimary (citable) accession number: B9DN65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3