SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B9DMT6

- DAGK_STACT

UniProt

B9DMT6 - DAGK_STACT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Diacylglycerol kinase
Gene
dagK, Sca_1470
Organism
Staphylococcus carnosus (strain TM300)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway By similarity.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Cofactori

Binds 1 magnesium ion per subunit. This ion appears to have a structural role and is required for catalytic activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATP By similarity
Binding sitei94 – 941ATP By similarity
Metal bindingi213 – 2131Magnesium; via carbonyl oxygen By similarity
Metal bindingi216 – 2161Magnesium By similarity
Metal bindingi218 – 2181Magnesium; via carbonyl oxygen By similarity
Active sitei273 – 2731Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 145ATP By similarity
Nucleotide bindingi67 – 737ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: InterPro
  3. diacylglycerol kinase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. phospholipid biosynthetic process Source: UniProtKB-KW
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSCAR396513:GJ9G-1517-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase
Short name:
DAGK
Gene namesi
Name:dagK
Ordered Locus Names:Sca_1470
OrganismiStaphylococcus carnosus (strain TM300)
Taxonomic identifieri396513 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000444: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 306306Diacylglycerol kinase
PRO_0000386499Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi396513.Sca_1470.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 132132DAGKc
Add
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.

Phylogenomic databases

eggNOGiCOG1597.
HOGENOMiHOG000261395.
KOiK07029.
OMAiYLKGMEM.
OrthoDBiEOG613098.

Family and domain databases

Gene3Di3.40.50.10330. 1 hit.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view]
PfamiPF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
TIGRFAMsiTIGR00147. TIGR00147. 1 hit.
PROSITEiPS50146. DAGK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B9DMT6-1 [UniParc]FASTAAdd to Basket

« Hide

MRKRARIIYN PTSGKELFKR MLPEVLVKME KAGFETSAYA TQKAGDATIE    50
SKRALQEDYE MLIVAGGDGT LNEVVNGIAE HPKRPKIGVI PMGTVNDFGR 100
ALHLPTDILK AVDVIIEGHS VKVDIGKMNS RYFINLAAGG RITEVSYETS 150
SKLKTFVGPF AYYIKGMEML PQMTNVDVRI EYDGQVFQGE ILLFLLGLTN 200
SMAGFEKLVP DARLDDGYFT LIIVQKANLA ELGHIMTLAS RGEHIKHPKV 250
IYEKAKSVNI SSFEQMPLNV DGEYGGQLPA NFLNLKQHIE VYTPKDVKNT 300
ELIQQD 306
Length:306
Mass (Da):34,082
Last modified:March 24, 2009 - v1
Checksum:iD47A72A4D371A18F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM295250 Genomic DNA. Translation: CAL28375.1.
RefSeqiYP_002634560.1. NC_012121.1.

Genome annotation databases

GeneIDi7550645.
KEGGisca:Sca_1470.
PATRICi19603784. VBIStaCar105558_1469.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM295250 Genomic DNA. Translation: CAL28375.1 .
RefSeqi YP_002634560.1. NC_012121.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 396513.Sca_1470.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 7550645.
KEGGi sca:Sca_1470.
PATRICi 19603784. VBIStaCar105558_1469.

Phylogenomic databases

eggNOGi COG1597.
HOGENOMi HOG000261395.
KOi K07029.
OMAi YLKGMEM.
OrthoDBi EOG613098.

Enzyme and pathway databases

BioCyci SCAR396513:GJ9G-1517-MONOMER.

Family and domain databases

Gene3Di 3.40.50.10330. 1 hit.
InterProi IPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR005218. Diacylglycerol/lipid_kinase.
IPR001206. Diacylglycerol_kinase_cat_dom.
[Graphical view ]
Pfami PF00781. DAGK_cat. 1 hit.
[Graphical view ]
SMARTi SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
TIGRFAMsi TIGR00147. TIGR00147. 1 hit.
PROSITEi PS50146. DAGK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome analysis of the meat starter culture bacterium Staphylococcus carnosus TM300."
    Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C., Goetz F.
    Appl. Environ. Microbiol. 75:811-822(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TM300.

Entry informationi

Entry nameiDAGK_STACT
AccessioniPrimary (citable) accession number: B9DMT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi